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Construction method of laver mutant library

A construction method and mutant technology, applied in the field of plant breeding, can solve problems such as difficulty in obtaining mutant germplasm

Pending Publication Date: 2021-09-07
OCEAN UNIV OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Macroalgae are important aquaculture species and have important economic value. However, compared with higher crops, there are fewer studies on the construction of seaweed mutant libraries, which brings certain difficulties to the acquisition of mutants and germplasm selection.

Method used

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  • Construction method of laver mutant library
  • Construction method of laver mutant library
  • Construction method of laver mutant library

Examples

Experimental program
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Effect test

Embodiment 1

[0041] Material: 30-day-old Porphyra zebra RZ pure line fronds cultivated in the laboratory, about 5 cm long. Culture conditions: temperature 10°C, light intensity 35-45μmol photons m -2 the s -1 , Light cycle / dark cycle: 12h / 12h, seawater salinity 27P.S.U, replace the seawater containing PES medium every 3 days.

[0042] Reagent: ethyl methanesulfonate (Ethyl Methanesulfonate, EMS) volume fraction is 0, 1%, 1.5%, 2.25%, 3.375%, 5.0625%; mass fraction is 5% sodium thiosulfate solution; sterilized seawater

[0043] Equipment: inverted microscope, centrifuge, 500mL Erlenmeyer flask, 50mL centrifuge tube, blade, glass slide, cover slip, 1.5mL centrifuge tube, 12-well plate, counter, counting plate, pipette (5mL, 1mL, 50μL ), gun tip

[0044] A method for constructing a laver mutant library, the steps are as follows:

[0045] 1. Prepare laver spores:

[0046] ①Chop 0.1g of healthy immature laver on a glass slide to fragments of 20-30 cells; transfer the fragments to a 1.5mL e...

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Abstract

The invention discloses a construction method of a laver mutant library, and belongs to the technical field of plant breeding. According to the construction method of the laver mutant library, the mutant library with high mutation saturation is obtained by taking porphyra yezoensis monospores as a mutagenic material and ethyl methanesulfonate as a mutagenic agent and optimizing mutagenic conditions, so that the mutant library has mutants with various characters, the obtained mutagenic progeny is a pure line and is relatively high in mutation rate, and abundant germplasm resources are provided for germplasm breeding and genetic research of the laver.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for constructing a porphyra mutant library. Background technique [0002] Mutant library construction methods can be divided into three categories: spontaneous mutation, physical and chemical mutagenesis and molecular biology methods. The frequency of spontaneous mutations is low, only 7.4×10 in rice and Arabidopsis -9 and 7×10 -9 , and the genetic background is not clear, which cannot meet the high-throughput requirements of modern biological research. Physical and chemical mutagenesis is the most commonly used method. It is easy to operate, has a high mutation rate, and has relatively large mutation randomness. Commonly used mutagens are 60 Co-γ rays, ethyl methanesulfonate, sodium azide, etc. The mutagenesis operation of molecular biology methods is relatively complicated and requires a mature genetic transformation system. Commonly used methods...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06A01H3/04A01H13/00A01G33/02
CPCA01H1/06A01H3/04A01H13/00A01G33/00
Inventor 孔凡娜孙斌殷吉强茅云翔
Owner OCEAN UNIV OF CHINA
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