2 results about "Spontaneous mutation" patented technology

The present application belongs to the field of metabolic engineering, and particularly relates to a genetically engineered strain for producing L-homoserine, a preparation method and application thereof. The present application utilizes directed evolution, and through continuous enrichment of spontaneous mutation of homoserine-producing bacteria SH017, a strain SHD275 for efficiently fermenting and producing L-homoserine is screened. The fermentation yield and sugar conversion rate of the production strain are both higher than those of the original strain SH017, thereby greatly saving the production cost.

The twitcher mouse model is a main animal model for researching the pathogenesis and treatment method of GLD disease at present, and the model is caused by a spontaneous mutation of a C57BL / 6 mouse model GALC At present, a commonly used genotype identification method is time-consuming and expensive. In order to overcome the defect, the special identification primer is designed, the PCR method is adopted, and the restriction endonuclease Eco RV is used for single enzyme digestion verification, so that a rapid, efficient and economic genotype identification method is established, and a reference for the genotype identification of the twitcher mouse is provided.