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Cordyceps militaris rejuvenation breeding process

A technology of Cordyceps militaris and a process is applied in the field of Cordyceps militaris rejuvenation breeding technology, which can solve the problems of slowing down the growth rate of mycelium, reducing the yield of metabolites, reducing the formation rate of fruiting bodies, etc. The effect of increased entity formation rate

Inactive Publication Date: 2019-03-29
LIAONING FUYUDA AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] In the process of industrialized cultivation of Cordyceps militaris, how to solve the decline of strains, the growth rate of mycelium slows down, the mycelium is sparse, the amount of powdery spores increases, the rate of fruiting body formation decreases or the fruiting bodies are short and deformed, and during the fermentation process, all Issues such as the substantial reduction in the output of metabolites have become key issues in the factory cultivation of Cordyceps militaris

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A kind of Cordyceps militaris rejuvenation breeding technique, concrete steps are as follows:

[0028] (1) Inoculate the degenerated Cordyceps militaris Hongqiao 1# strain in PDA slant medium, and carry out activation culture at 23°C for 8 days; the PDA slant medium is: potato 200g / L, sucrose 20g / L, agar 15g / L , PH natural.

[0029] (2) Inoculate the activated Cordyceps militaris Hongqiao 1# bacterial strain in the shake flask medium, and cultivate it for 3 days at 23°C; the seed medium is: potato 200g / L, sucrose 20g / L, potassium dihydrogen phosphate 3g / L L, magnesium sulfate 1g / L, peptone 3g / L, VB 1 It is 10mg / L.

[0030] (3) Inoculate the cultivated shake flask seed solution on the wheat or rice cultivation medium, and cultivate at 20°C for about 40 days; the cultivation medium is: wheat or rice 30g / bottle, water 40ml / bottle.

[0031] (4) Select eight-mature fruiting bodies with good shape and strong growth, intercept the tip and hang it on the bottle cap of the cu...

Embodiment 2

[0034] A kind of Cordyceps militaris rejuvenation breeding technique, concrete steps are as follows:

[0035] (1) Inoculate the degenerated Cordyceps militaris Hongqiao 1# strain in PDA slant medium, and carry out activation culture at 23°C for 8 days; the PDA slant medium is: potato 200g / L, sucrose 20g / L, agar 15g / L , PH natural.

[0036] (2) inoculate the Hongqiao 1# strain of Cordyceps militaris after activation in the shake flask medium, and cultivate it at 23° C. for 3.5 days; the seed medium is: potato 200 g / L, sucrose 20 g / L, potassium dihydrogen phosphate 3 g / L, magnesium sulfate 1g / L, peptone 3g / L, VB 1 10mg / L.

[0037] (3) Inoculate the cultivated shake flask seed solution on wheat or rice cultivation medium, and cultivate at 21°C for about 42 days; the cultivation medium is: wheat or rice 35g / bottle, water 50ml / bottle.

[0038] (4) Select eight-mature fruiting bodies with good shape and strong growth, intercept the tip and hang it on the bottle cap of the cultiv...

Embodiment 3

[0041] A kind of Cordyceps militaris rejuvenation breeding technique, concrete steps are as follows:

[0042] (1) Inoculate the degenerated Cordyceps militaris Hongqiao 1# strain in PDA slant medium, and carry out activation culture at 23°C for 8 days; the PDA slant medium is: potato 200g / L, sucrose 20g / L, agar 15g / L , PH natural.

[0043] (2) Inoculate the activated Cordyceps militaris Hongqiao 1# bacterial strain in the shake flask culture medium, and cultivate it at 23°C for 4 days; the seed culture medium is: potato 200g / L, sucrose 20g / L, potassium dihydrogen phosphate 3g / L L, magnesium sulfate 1g / L, peptone 3g / L, VB 1 10mg / L.

[0044] (3) Inoculate the cultivated shake flask seed solution on the wheat or rice cultivation medium, and cultivate at 22°C for about 45 days; the cultivation medium is: 40g / bottle of wheat or rice, and 55ml / bottle of water.

[0045](4) Select eight-mature fruiting bodies with good shape and strong growth, intercept the tip and hang it on the b...

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Abstract

The invention discloses a cordyceps militaris rejuvenation breeding process. The concrete steps are as follows: carrying out activation culture of a degraded cordyceps militaris strain; inoculating the activated cordyceps militaris strain in a shake flask culture medium for culturing, and the inoculating the strain on a wheat or rice culture medium for culturing; selecting fruiting bodies, cuttingoff tips, hanging the tips on the cup of the cultivation culture medium for culturing until spores on the surface of the culture medium are germinated thoroughly, removing the fruiting body tips, andcontinuing to culture the tips for 30-40 d; and picking and drying the mature cordyceps militaris, detecting cordycepin, the main active ingredient of the cordyceps militaris, and using the cordycepsmilitaris as an original cultivation species until the traits are stable. The cordyceps militaris rejuvenation breeding process does not need to use a spore collector, is simplified in operation andsaves breeding cost. Furthermore, since the process is monospore purification rejuvenation, the purity of the strain can be guaranteed. The excellent strains with stable traits are orientedly screenedas the original cultivation species, and are suitable for the rejuvenation of strains degraded by spontaneous mutation and inappropriate environmental factors.

Description

technical field [0001] The invention relates to the technical field of rejuvenation of degenerated edible fungus strains, in particular to a rejuvenation breeding process of Cordyceps militaris. Background technique [0002] Cultivation of edible fungi requires high yield, and the strain is the key. Cordyceps militaris is a model species of Ascomycota, Ergot, Ergotaceae and Cordyceps. Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, is a related species of Cordyceps sinensis. Its effective components are similar to those of Cordyceps sinensis, containing nucleosides, polysaccharides, sterols and SOD enzymes, etc., and the content of cordycepin is even higher. In Cordyceps sinensis, it has various pharmacological effects such as improving the body's immunity, anti-tumor, anti-aging, protecting heart and lung tissue, sedative and hypnotic. Therefore, Cordyceps militaris is used in health care medicine and food as a substitute of Cordyceps sinen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00
Inventor 王弘
Owner LIAONING FUYUDA AGRI SCI & TECH
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