A strain of anti-phage aspartase variant production bacteria and its selection method and application

Abstract

The invention discloses an anti-bacteriophage aspartase variant production bacterium, a breeding method and an application thereof. The present invention carries out continuous spontaneous mutation breeding on the aspartase variant producing bacteria pL181 which is easily polluted by phages, and then utilizes the low-energy ion implantation mutagenesis method to efficiently breed a strain of aspartase with phage resistance Acidase variant producing bacteria Escherichia coli CGMCC NO.18005. The strain not only has the resistance to bacteriophage (CGMCC NO.17999), but also has 31% higher enzyme activity than the original strain pL181. While improving the enzyme-producing ability of the producing bacteria, the present invention fundamentally solves the difficult problem that β-alanine is easily infected by phages in the production, and improves the economic benefits of β-alanine production enterprises.

Description

technical field

[0001] The invention belongs to the technical field of bioengineering, and in particular relates to a bacteriophage-resistant aspartase variant-producing bacterium, a breeding method and its application. Background technique

[0002] β-alanine, also known as 3-alanine, is the only β-type amino acid that exists in nature. The physiological function of β-alanine is mainly as an intermediate product of metabolism, which widely exists in the metabolic process of animals, plants and microorganisms, and plays an essential role in the growth and development of organisms. It is mainly used in the synthesis of pantothenic acid and calcium pantothenate, carnosine, sodium pamidronate, balsalazide, etc., and is widely used in medicine, feed, food and other fields. In recent years, foreign reports on β-alanine have gradually increased, most of which are studies on β-alanine derivatives. The role of derivatives of β-alanine in medical treatment, environment and daily che...

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