Stress, signaling and transcriptional regulatory genes involved in regulating act production in Streptomyces

Abstract

The application belongs to the technical field of microbial fermentation engineering, and specifically relates to genes related to the production of polyketide actin (ACT) in streptomyces. This application specifically relates to the application of stress, signal and transcription regulation genes in the regulation of Streptomyces ACT production. There are 14 stress, signal and transcription regulation genes in total; mutations of such genes have regulatory effects on the ACT production of Streptomyces. The inventor carried out mutagenesis on Streptomyces coelicolor M145, and screened the strains with changed actin rhodopsin production. The position of the transposon on the genome was located by enzyme digestion, self-ligation and sequencing of the screened positive clones, and the principle of multiple screening to locate the same gene was used to exclude the spontaneously mutated gene, and finally determine the factor affecting the production of actinhodine. Gene.

Description

technical field [0001] The present application belongs to the technical field of microbial fermentation engineering, and specifically relates to a gene related to the production of polyketide ACT (ACT) in Streptomyces sp. Background technique [0002] Existing studies generally believe that small molecular compounds produced by microbial secondary metabolism are of great significance to human health. Statistics show that more than 60% of FDA-approved anti-infective and anti-tumor drugs are derived from microbial secondary metabolites. With more and more microbial genome sequencing work, genome sequence analysis shows that microorganisms have great potential to produce various secondary metabolites, but some studies have shown that many microbial secondary metabolites cannot be expressed under laboratory culture conditions. , and most environmental microorganisms cannot be effectively cultured under laboratory conditions, thus seriously hindering the development of microbial...

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Problems solved by technology

[0004] In the prior art, the inventors used transposition mutagenesis technology to study the functional genome of Streptomyces coelicolor A3(2), and initially constructed a mutant library of Streptomyces coelicolor A(3)2 (for details, please refer to : Xu Zhong, using transposition mutagenesis to study the functional genome of Streptomyces coelicolor A3 (2), Master thesis of Huazhong Agricultural University in 2011), but due to the complexity and uncertainty of the regulatory functions of related genes, which specific genes are related to The regulation of secondary metabolites still needs further research and discussion

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