SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker

A technology of molecular markers and pig backfat thickness, applied in the field of molecular genetics, can solve the problems of lagging functional research and inaccurate gene positioning, etc., and achieve excellent economic value, low cost, and adapt to a wide range of pig breeds

Active Publication Date: 2016-01-20
湘潭市家畜育种站
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  • Summary
  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

The functional research of MARK4 and its family members in the mouse model is relatively thorough, but the function in pigs has not been reported so far. From the applicant's previous research, the genome segment where the MARK4 gene is

Method used

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  • SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker
  • SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Obtaining of SNP molecular markers related to pig backfat thickness traits

[0031] Taking 268 Duroc pigs with complete phenotype data as the research object, other Chinese local pig breeds (with no relatives among individuals) include 33 Jinhua pigs, 25 Neijiang pigs, 13 Erhualian pigs, 32 Wuzhishan pigs, 29 Bamei pigs and 26 Tibetan pigs are high-backfat breed groups; 30 Large White pigs and 29 Landrace pigs are low-backfat breed groups. For the above verification, some bases of the pig MARK4 gene segment were sequenced by PCR technology Sequences, supplemented with partial genome sequences.

[0032] According to the predicted porcine MARK4 gene sequence (XM_005658175) and human MARK4 gene sequence (NM_001199867) published by NCBI, primers were designed to amplify the porcine MARK4 gene mRNA sequence. fragments.

[0033] Table 1 MARK4 gene SNP detection specific primers

[0034]

[0035] According to reverse transcription PCR technology and RACE (Rapid-a...

Embodiment 2

[0037] Example 2 Establishment of a method for detecting SNP molecular markers related to pig backfat thickness traits

[0038] 1. Use RFLP restriction endonuclease to detect the population level of SNP sites

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Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related with a back fat thickness trait of a pig and a detection method of the SNP molecular marker. By detecting exon regions of MARK4 (Microtubule Affinity-Regulating Kinase) genes of different pig species, one SNP locus (G-A) is found to exist at 55th basic group (mRNA (Messenger Ribonucleic Acid) total-length 2581th base group) in 3 minute UTR (Untranslated Region) of the MARK4 gene, an allele A is positively correlated with low back fat trait, and an allele G is positively correlated with high back fat trait. The invention provides the method for detecting the SNP molecular marker. Pig genomic DNA (Deoxyribonucleic Acid) to be detected is amplified by using a primer pair with the nucleotide sequence shown in SEQ ID NO.1-2 and is subjected to enzyme digestion by using pstI; if an enzyme-digested product is 251bp, a base group at a mutation is A; if the enzyme-digested product is 218bp, a base group at the mutation is G. According to the method provided by the invention, the back fat thickness of the pig can be predicted early, quickly and effectively at low cost; the SNP molecular marker can be used as a useful molecular marker for genetic improvement of the pig species.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to molecular markers related to pig backfat traits, in particular to SNP molecular markers related to pig backfat traits, applications thereof and methods for detecting the molecular markers. Background technique [0002] Throughout the world, the highest proportion of pork consumption in the total meat consumption is about 37%, and the total pork consumption is still increasing at an annual rate of about 1.1% between 2012 and 2014 (http: / / www.fao.org / ag / againfo / themes / en / meat / background.html). China is a big country in pork production and consumption in the world. According to statistics, the number of live pigs produced and consumed in China accounts for almost half of the world's total consumption (http: / / www.economist.com / news / christmas-specials / 21636507-chinas- insatiable-appetite-pork-symbol-countrys-rise-it-also, 2014 / 12 / 20). However, with the improvement of people's livi...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 方美英王克君白莹刘成琨许桥
Owner 湘潭市家畜育种站
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