SNP (Single Nucleotide Polymorphism) molecular marker related with back fat thickness trait of pig and detection method of SNP molecular marker

Abstract

The invention provides an SNP (Single Nucleotide Polymorphism) molecular marker related with a back fat thickness trait of a pig and a detection method of the SNP molecular marker. By detecting exon regions of MARK4 (Microtubule Affinity-Regulating Kinase) genes of different pig species, one SNP locus (G-A) is found to exist at 55th basic group (mRNA (Messenger Ribonucleic Acid) total-length 2581th base group) in 3 minute UTR (Untranslated Region) of the MARK4 gene, an allele A is positively correlated with low back fat trait, and an allele G is positively correlated with high back fat trait. The invention provides the method for detecting the SNP molecular marker. Pig genomic DNA (Deoxyribonucleic Acid) to be detected is amplified by using a primer pair with the nucleotide sequence shown in SEQ ID NO.1-2 and is subjected to enzyme digestion by using pstI; if an enzyme-digested product is 251bp, a base group at a mutation is A; if the enzyme-digested product is 218bp, a base group at the mutation is G. According to the method provided by the invention, the back fat thickness of the pig can be predicted early, quickly and effectively at low cost; the SNP molecular marker can be used as a useful molecular marker for genetic improvement of the pig species.

Description

technical field [0001] The invention relates to the field of molecular genetics, in particular to molecular markers related to pig backfat traits, in particular to SNP molecular markers related to pig backfat traits, applications thereof and methods for detecting the molecular markers. Background technique [0002] Throughout the world, the highest proportion of pork consumption in the total meat consumption is about 37%, and the total pork consumption is still increasing at an annual rate of about 1.1% between 2012 and 2014 (http: / / www.fao.org / ag / againfo / themes / en / meat / background.html). China is a big country in pork production and consumption in the world. According to statistics, the number of live pigs produced and consumed in China accounts for almost half of the world's total consumption (http: / / www.economist.com / news / christmas-specials / 21636507-chinas- insatiable-appetite-pork-symbol-countrys-rise-it-also, 2014 / 12 / 20). However, with the improvement of people's livi...

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Problems solved by technology

The functional research of MARK4 and its family members in the mouse model is relatively thorough, but the function in pigs has not been reported so far. From the applicant's previous research, the genome segment where the MARK4 gene is located is exactly the pig genome in the NCBI database The unfinished region of the gene has not been accurately positioned on the pig genome, and has also caused the functional research of this gene in pigs to lag behind other species.

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