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Molecular identification method of atlantic salmon

A salmon and molecular technology, applied in the molecular identification of Atlantic salmon and the authenticity identification of Atlantic salmon, can solve the problems of high price, unreported, time-consuming, etc., and achieve low price, low equipment requirements, and simple operation. Effect

Active Publication Date: 2013-05-29
YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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  • Abstract
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  • Application Information

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Problems solved by technology

In comparison, DNA barcode identification technology has advantages over other identification technologies, but it strongly relies on sequencing technology. In China, it needs to be sent to a specialized sequencing company, which is expensive and time-consuming
Therefore, the market is in urgent need of a molecular identification method for Atlantic salmon that is simple to operate, fast and accurate, cheap and requires low equipment requirements, but there is no report in the prior art

Method used

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  • Molecular identification method of atlantic salmon
  • Molecular identification method of atlantic salmon
  • Molecular identification method of atlantic salmon

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Embodiment Construction

[0019] A molecular identification method for Atlantic salmon, the steps are as follows:

[0020] 1. Fish DNA extraction:

[0021] Weigh 100 mg of fish meat with an electronic balance into a 1.5 mL sterilized centrifuge tube, add 400 μL of TE extract containing 1% CTAB, 0.05 mol / L Tris-HCl at pH 8.0, 0.7 mol / L L NaCl, 0.01mol / L EDTA at pH8.0, cut the fish tissue with scissors sterilized with 70% alcohol, then add 40 μL of 10% SDS to a final concentration of 1.9-2.1%, and add 8 μL of 20 mg / ml proteinase K to a final concentration of about 400μg / L, shake up and down, mix thoroughly, put the centrifuge tube in a constant temperature water bath at 55-65℃, until the mixture is digested until clear; take out the digested sample, add 300μL 6M saturated NaCl solution, vortex at high speed Spin for 30s, centrifuge at 12000r / min for 30min, take the supernatant into a new 1.5ml centrifuge tube, add an equal volume of a mixture of phenol, chloroform and isoamyl alcohol, the mixing ratio i...

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Abstract

The invention discloses a molecular identification method of an atlantic salmon. The molecular identification method comprises the following steps of 1, extraction of fish DNA (deoxyribonucleic acid); 2, PCR (polymerase chain reaction) augmentation; 3, enzyme digestion and electrophoresis detection; and 4, identification. The atlantic salmon can be accurately determined in four steps. The molecular identification method of the atlantic salmon has the characteristics of being simple to operate, rapid and accurate, low in price, low in requirement on an instrument and equipment, and the like.

Description

technical field [0001] The invention relates to a molecular identification method of Atlantic salmon, which belongs to the field of biomolecular detection and is mainly used for authenticity identification of Atlantic salmon. Background technique [0002] Atlantic salmon is usually called Norwegian salmon, because Atlantic salmon sold in the Chinese market is mainly imported from Norway and other countries. Compared with other salmon, Atlantic salmon has better quality and higher price. Salmon is not a fish classification name, but a trade name for certain salmonids or salmon trout, usually referring to a cold-water fish that grows in high latitudes such as Canada, Norway, Japan and the United States, common salmon There are salmon (Oncorhynchus keta), rainbow trout (Oncorhynchus mykiss), coho (Oncorhynchus kisutch), sockeye (Oncorhynchus nerka) and Atlantic salmon (Salmo salar). [0003] In recent years, the trade scale of salmon has grown rapidly. The trade volume has inc...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 周德庆李新光赵峰马丽萍孙永
Owner YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI
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