127 results about "Porphyra dioica" patented technology

The invention belongs to molecular biology DNA labeling technique and application field, concretely relates to a porphyra yezoensis microsatellite marker screening method, and a method for analyzing germplasm genetic diversity by the makers, thereby lay foundations for genetic structure analysis of porphyra yezoensis, germplasm resource protection and molecular marker auxiliary breeding.

The invention discloses a method and device for automatically washing picked porphyra yezoensis shoots. The device for automatically washing the picked porphyra yezoensis shoots comprises a submerged pump and a water inlet pipe. The water inlet pipe is shaped like a Chinese character 'hui'. One end of the water inlet pipe is connected with the submerged pump and the other end of the water inlet pipe is provided with three arm type rotary nozzles. The interval of the nozzles arranged on the same line ranges from 1.5 m to 4 m. Two rows of parallel nozzles are arranged in a staggered mode. The spraying range of the nozzles is a circle with the radius ranging from 1 m to 2 m. Each nozzle is composed of a hollow shaft core and three arm type water outlet pipes. The hollow shaft cores are connected with the water inlet pipe. Water outlets are evenly formed in the arm type water outlet pipes. After stimulation of the day and night temperature difference and the running water stimulation, conchospores are diffused by shell protonemata in a concentrated mode in the morning of a next day, high-density conchospore water in a nursery pond is pumped to the water inlet pipe through the submerged pump and sprayed to a shoot picking net curtain through the three arm type rotary nozzles, and therefore a whole nursery system is washed synchronously and evenly. The device is simple in structure and operation, quite low in constructive cost, low in use cost and better in shoot picking effect.

The invention relates to an application of a seaweed polypeptide in preparing a health-care product for reducing blood fat and blood sugar as well as a beverage containing the polypeptide for reducing the blood fat and the blood sugar. The seaweed polypeptide is prepared by the following steps of: milling a mixture of immersed porphyra haitanensis and water through a colloid mill to obtain porphyra haitanensis slurry; adopting AS.1398 neutral protease to carry out enzymolysis on the porphyra haitanensis slurry for 5-10 hours under the conditions that the temperature is 45-55 DEG C, the pH (Potential of Hydrogen) is 7.2-7.8, the enzyme adding amount E/S is 8000-12000 U/g and the weight/volume ratio concentration of a substrate is 3-7%, so as to obtain an enzymolysis solution; centrifuging the enzymolysis solution and collecting liquid supernatant; and carrying out ultra-filtering separation on the liquid supernatant to obtain an active component with the molecular weight of less than 10000 Da. An animal experiment shows that agars are subjected to biological enzymolysis to obtain the seaweed polypeptide which has the obvious effects of reducing the blood fat, the blood sugar and blood pressure; and after the seaweed polypeptide is taken for a long period, the weight can be prevented from too fast increase and the happening possibility of arteriosclerosis can be reduced.

The invention belongs to the technical field of plant growth conditioning agents, and relates to a plant salt resisting conditioning agent. The plant salt resisting conditioning agent is prepared from porphyra polysaccharides with the concentration of 100-1,000 mg/L, salicylic acid with the concentration of 0 mg/L or 100-200 mg/L, brassinolide with the concentration of 0 mg/L or 0.01-0.1 mg/L, glycine betaine with the concentration of 0 mg/L or 100-1,000 mg/L, spermidine with the concentration of 0 mg/L or 10-100 mg/L, a surfactant with the concentration of 0 mg/L or 1,000-20,000 mg/L and water, wherein the water serves as a solvent, and low-molecular-weight porphyra polysaccharides with the molecular weight of 1 k-50 k Da are adopted as the porphyra polysaccharides. Accordingly, novel application of the porphyra polysaccharides is supplied, and the porphyra polysaccharides can be used for preparing the plant salt resisting conditioning agent; meanwhile, by compounding the porphyra polysaccharides with the biological source induced resisting substances such as salicylic acid, brassinolide, glycine betaine and spermidine to prepare the salt resisting conditioning agent, the comprehensive resistance of plants on salt stress can be improved, and normal growth and development of the plants are guaranteed; according to the salt resisting conditioning agent, the components are simple, the raw materials are easy to obtain, the cost is low, the effect is significant, and environmental friendliness is achieved.

The invention provides a preparation method and application of a low-molecular porphyra haitanensis polyferose compound. The method is characterized in that low-molecular porphyran is used as a raw material and degraded by redox reaction to obtain low-molecular-weight porphyran with the molecular weight being less than 10000Da; and the polyferose compound synthesized by heating the porphyran and ferric chloride together can be dissolved well and exist stably under a physiological pH condition, and also shows a good effect in the iron-deficiency anemia mouse model experiment. The preparation method has the advantages of being simple and convenient in operation, stable in preparation process, low in preparation cost, etc.; and the preparation method also shows wide applicability to raw materials, namely, any polysaccharide with a proper molecular weight range is applicable to the preparation method.

The invention belongs to the field of storage of aquatic products and particularly relates to a preservative and a method for preserving fresh prawns. The preservative is a mixed solution of blueberry leaf polyphenol, porphyra polysaccharide and citric acid, wherein the concentration of the blueberry leaf polyphenol is 2-8 mg/mL, the concentration of the porphyra polysaccharide is 2-10 mg/mL, the concentration of the citric acid is 0.002-0.02 mg/mL, and the rest is water. The blueberry leaf polyphenol and the porphyra polysaccharide are quickly mixed, and meanwhile, deionized water is added and is uniformly stirred, and then, a proper amount of citric acid is added, and then, the fresh prawns are preserved. According to the preservative provided by the invention, the blueberry leaf polyphenol and the porphyra polysaccharide are used for well preserving the fresh prawns without generating food safety problem, and meanwhile, no high cost is needed.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg/L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The invention relates to a porphyra haitanensis polysaccharide as well as a preparation method and probiotic activity application thereof. Porphyra haitanensis is used as a raw material and is extracted through a traditional hot water extraction method; after the porphyra haitanensis is precipitated through ethanol, obtained polysaccharide sediment is re-dissolved into a low-concentration salt solution; after dialysis and freeze drying are carried out, the porphyra haitanensis polysaccharide with greatly improved solubility and viscosity is obtained; the effect of regulating intestinal flora of human bodies is easier to realize and the immunity is improved. The polysaccharide can be applied to preparation of medicines and/or foods with probiotic activity. The extracted porphyra haitanensispolysaccharide is added into yogurt to prepare yogurt chewable tablets; the obtained polysaccharide yogurt chewable tablets integrate effects of prebiotics and probiotic bacteria; the production costis low and the polysaccharide yogurt chewable tablets have no side effect on the human bodies; the polysaccharide yogurt chewable tablets have the effects of regulating the intestinal flora of the human bodies and enhancing the immunity of organisms, are convenient to carry and is convenient for oral administration.

The invention discloses a method for separating an alpha-glucosidase inhibitor from a laver enzymolysis product, which belongs to the technical field of separation and purification of biological products. The invention relates to a method for compounding porphyra yezoensis to obtain a rough alpha-glucosidase inhibitor product, wherein the enzymolysis is controllable and the cost is low. The rough product is separated and purified through SPSepharoseHighPerformance cation exchange chromatography, SephadexG-10 gel chromatography and Mono Q anion exchange chromatography, and the purity and main composition of an LGI (lactate glucose index) part with high inhibitory activity on alpha-glucosidase are analyzed through reversed phase high performance liquid chromatography and ultra-high performance liquid chromatography-mass spectrography. The IC 50 value of LGI on the alpha-glucosidase is 97.63mu g/ml, and the temperature and pH stability are good; the inhibitory activity is kept to be higher than 80% in 80 DEG C water bath for 7 h, and the inhibitory activity is still kept to be higher than 87% after being maintained in a buffer solution with pH of 3-12 at 50 DEG C for 2 h. The LGI is small in molecular weight, good in stability, low in cost and safe to use, thereby having a quite wide application prospect in such fields as medicine, food, etc.

The invention discloses rice flour for preventing Alzheimer's disease. The rice flour is prepared from the following components in parts by weight: 10 to 15 parts of food composition, 60 to 80 parts of rice meal, 12 to 18 parts of malt flour, 10 to 15 parts of skim milk powder, 5 to 8 parts of multivitamin and 5 to 10 parts of a natural sweetener, wherein the food composition is prepared from the following components in parts by weight: 10 to 20 parts of garlic extract, 5 to 15 parts of porphyra haitanesis extract and 2 to 8 parts of radix puerariae extract; the rice flour is prepared by mixing the components and then carrying out nanometer crushing treatment on the mixture. The rice flour disclosed by the invention is formed by scientific and reasonable matching and has the advantages of reasonable proportion, high pertinence, good mouth feel, convenience in eating, easiness in digestion and absorption, good instant solubility and good reconstituability; in addition, the rice flour has the efficacy of delaying senility effectively, preventing the Alzheimer's disease and the like, and is quite suitable for middle-aged and elderly people to eat.

The NR enzymes described herein were discovered in the red algae of Porphyra perforata (PpNR) and Porphyra yezoensis (PyNR). The present invention provides methods and compositions relating to altering NR activity, nitrogen utilization and/or uptake in plants. The invention relates to a method for the production of plants with maintained or increased yield under low nitrogen fertility. The invention provides isolated nitrate reductase (NR) nucleic acids and their encoded proteins. The invention further provides recombinant expression cassettes, host cells, and transgenic plants. Plants transformed with nucleotide sequences encoding the NR enzyme show improved properties, for example, increased yield and growth.

The invention provides porphyra metal-chelating protein peptide and a preparation method thereof. The amino acid sequence of peptide is eagdsqekv. The preparation method comprises the following steps: by taking porphyra protein as a raw material, compounding composite protease and flavourzyme for enzymolysis of porphyra protein, and performing separation purification and freeze drying, thereby obtaining metal-chelating protein. On the theoretical basis that polypeptide has action sites for chelating with metal ions and can form a stable compound with metal ions, and a polypeptide-metal chelate has unique chelating mechanisms and operation mechanisms, can be easily absorbed and can simultaneously supplement amino acid and metal, peptide with high metal (Ca, Fe and Zn) chelating activity can be prepared through cutting, and the metal-chelating activity can be efficiently brought into play.

The invention discloses angiotensin converting enzyme (ACE) inhibitory peptide which has specific sequence and is screened from porphyra haitanensis enzymolysis products. The sequence of the polypeptide is Thr-Gly-Ala-Pro-Cys-Arg (TGAPCR), and through mass spectrum identification, the molecular weight of the polypeptide is 603.69 Da. The polypeptide has the characteristics of stable structure, notoxicity, high water solubility and the like; and through in-vitro high-performance liquid chromatography (HPLC) identification, the polypeptide has low IC50. Significant effect of reducing the systolic pressure of spontaneously hypertensive rats in vivo is achieved; and the diastolic pressure can be reduced for a long time. The ACE inhibitory peptide with specific sequence can be applied to development of an ACE inhibitor and also can be applied to health-care products and medicine products related to hypertensive therapy.

The invention discloses a thamnaconus modestus fish skin nanoparticle composite edible membrane which comprises the following components in parts by weight: 33-40 parts of gelatin, 0.65-1.2 parts of porphyra haitanensis antioxidant, 6.5-1 part of plasticizer and 55-60 parts of chitosan microsphere. The technology comprises the steps of preparing thamnaconus modestus fish skin gelatin, preparing chitosan nanoparticle and preparing porphyra haitanensis antioxidant, then preparing the prepared gelatin, chitosan nanoparticle and porphyra haitanensis antioxidant into a solution, and preparing a film-forming solution by the addition of the plasticizer, finally pouring the prepared film-forming solution on a polyvinyl chloride crystal board for striking, drying and film uncovering to obtain the antioxidant gelatin-chitosan nanoparticle composite edible membrane. The invention takes the thamnaconus modestus fish skin as the main raw material, so that a new approach for high-value use of the thamnaconus modestus processing leftovers is established; the prepared antioxidant gelatin-chitosan nanoparticle composite edible membrane has remarkable antioxidant activity and seaweed flavor.

The invention discloses a brain-tonifying and qi-boosting coarse grain instant-dissolving product and a preparation method thereof. The product is composed of following raw materials including, by weight, 5-15 parts of buckwheat, 10-20 parts of corn, 5-10 parts of Chinese yam, 5-8 parts of cashew nuts and 3-7 parts of porphyra. The coarse grain instant-dissolving product is in the forms of an instant-dissolving powder, an instant-dissolving granule and an instant-dissolving sandwiched multilayer granule. In the product, the buckwheat, the corn, the Chinese yam, the cashew nuts and the porphyra are reasonably matched, wherein the buckwheat and the corn are employed as staple food for providing energy for human body, the Chinese yam can fortify spleen and boost qi, the cashew nuts is significant in a brain tonifying effect, and the porphyra can supply various human-required microelements and amino acids. The product, when being often eaten, has a quite good brain-tonifying and qi-boosting health-caring effect.

The invention discloses an eye cream composition containing an anti-blue-light lipidosome and a preparation method thereof. The anti-blue-light eye cream is prepared from the following raw material components: 1.0-35.0% of an anti-blue-light lipidosome, 5.0-40.0% of an emollient, 0.5-30.0% of an emulsifier, 0.5-20.0% of a filler, 0.1-10.0% of a thickener, 0.3-5.0% of a preservative, 0.01-1.0% of daily essence and the balance deionized water. The eye anti-blue-light lipidosome is prepared by combining a rhodiola crenulata root extract, a porphyra umbilicalis extract and carnosine. Therefore, the liposome is prepared from the three anti-blue-light functional components and has better transdermal absorption and slow release effects. The anti-blue-light functional components have strong antioxidant ability, can remove free radicals generated by blue light irradiation, effectively delay skin aging caused by blue light, reduce pigmentation and stain generation, repair light loss skin, effectively maintain skin elasticity, make skin bright and uniform, keep skin young and vigor, and improve other aging phenomena of skin around eyes.

The invention relates to a complementary nutritional fine-dried noodle for a baby. The complementary nutritional fine-dried noodle comprises the following materials by weight percent: 85%-86% of organic wheat powder, 3.5-6.0% of porphyra powder, 1.5-2.5% of jujube powder, 2.5-3.0% of yolk powder and 1.0-3.5% of yam powder. The complementary nutritional fine-dried noodle is a pure-natural organic nutritional supplementary fine-dried noodle, pure-natural materials are adopted and are dried and crushed to obtain corresponding powder, and the nutritional components in the materials can be basically or wholly retained. The complementary nutritional fine-dried noodle is comprehensive and rich in nutrition and convenient to eat, is soft and tender after being cooked and is suitable for being used as complementary food for the baby with more than six months.

An anti-radiation tomato juice with green tea is prepared from following raw materials including, by weight, 100-120 parts of the tomatoes, 10-15 parts of the green tea, 300-320 parts of rice soup, 35-40 parts of flammulina velutipes, 50-60 parts of black sesame, 20-25 parts of porphyra powder, 6-8 parts of gynostemma pentaphyllum, 5-7 parts of gomphrena globosa and 5-7 parts of jasmine flowers. The anti-radiation tomato juice with green tea is prepared with addition of the tomatoes and the green tea, has a sour and sweet taste of the tomatoes and a refreshing fragrance of the green tea and has a certain anti-radiation effect. Through addition of extracts of the gynostemma pentaphyllum, the gomphrena globosa, the jasmine flowers, matricariarecutita and the like, the tomato juice with the green tea has good effects of eyesight improving and eye protecting, radiation resisting, fatigue eliminating and the like. The tomato juice with the green tea is suitable for people whom often work or live with computers and televisions.

The invention provides primary taste nori crisp, which is prepared from the following raw materials by a weight ratio: 32-35 parts of dry porphyra yezoensis and 65-70 parts of seasoning, wherein the seasoning comprises a main material and an auxiliary material, the main material is prepared from white sugar, glucose and corn starch according to a weight ratio of 7 to 3 to 1, and the auxiliary material is prepared from 2-4 parts of disodium 5'-ribonucleotide, 1.5-2 parts of vanillin, 10-16 parts of non-dairy creamer, 3-4 parts of citric acid, 5-10 parts of table salt and 1-3 parts of edible oil. The primary taste nori crisp provided by the invention is crisp in mouth feel, rich in nutrition, convenient to carry, economical and practical.