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Porphyra haitanensis polysaccharide as well as preparation method and probiotic activity application thereof

A technology of laver and polysaccharide, which is applied in the field of probiotics, polysaccharide of laver and its preparation, can solve the problems of high viscosity, poor solubility of polysaccharide, and industrial application restrictions, and achieve the advantages of convenient taking, growth inhibition, and high safety Effect

Active Publication Date: 2019-01-18
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a polysaccharide from Porphyra laver and its preparation method and the application of probiotics, so as to solve the problems of poor solubility and high viscosity of the current polysaccharide, and its industrial application is greatly restricted.

Method used

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  • Porphyra haitanensis polysaccharide as well as preparation method and probiotic activity application thereof
  • Porphyra haitanensis polysaccharide as well as preparation method and probiotic activity application thereof
  • Porphyra haitanensis polysaccharide as well as preparation method and probiotic activity application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Example 1: Preparation of laver polysaccharide PHP

[0035] After being exposed to the sun and pulverized by a pulverizer, the dried seaweed powder was weighed and mixed with an organic solution (methanol: dichloromethane: water = 4:2:1, v / v / v) according to a material-to-liquid ratio of 1:10 for decolorization. 12h, put the insolubles in a 50°C oven to dry to constant weight; add water according to the ratio of material to liquid 1:30, let stand for 30min at room temperature; use traditional hot water extraction method for extraction, extraction temperature 90°C, extraction time 2h; Heat centrifugation and collect the supernatant, slowly add 3 times the volume of 95% ethanol solution to it, let stand at 4°C overnight, collect the polysaccharide precipitate by centrifugation and redissolve it in 0.1mol / L NaCl solution, dialyze, concentrate, Freeze-drying, namely, obtaining the improved solubility of laver polysaccharide PHP.

[0036] Result: by figure 1 It can be seen ...

Embodiment 2

[0037] Example 2: Human intestinal flora fermentation PHP

[0038]Preparation of intestinal flora basal medium: 2.0g yeast extract, 2.0g peptone, 0.02g hemin, 0.5g L-cysteine, 0.5g bile salts, 2.0g NaHCO 3 , 0.1g NaCl, 0.04g KH 2 PO 4 ,0.04g K 2 HPO 4 ,0.01gCaCl 2 ·6H 2 O, 0.01 g MgSO 4 ·7H 2 O, 0.01g vitamin K 1 , 0.01g resazurin and 2.0mL Tween-80 were dissolved in 1.0L distilled water and sterilized at 121°C for 20min.

[0039] Preparation and fermentation of fecal inoculum: Four healthy volunteers (two men and two women, aged 20-22 years) with no history of gastrointestinal disease and no antibiotic treatment within two months were recruited. The collected fresh fecal samples were evenly dispersed in sterile anaerobic physiological saline (0.9%, w / v) to form a 10% (w / v) fecal suspension; centrifuged at 500 × g for 5 min to remove insoluble particles; Then, 1.0 mL of the fecal inoculum was injected into 9.0 mL of the basal medium. Among them, PHP group: containin...

Embodiment 3

[0040] Example 3: The effect of PHP on the composition of human intestinal flora

[0041] DNA extraction and PCR amplification: Add 1 ml of 0.1 mol / L PBS buffer to the pellet obtained after high-speed centrifugation to resuspend the bacteria. Total DNA from each fermentation sample was extracted with the QIA amp DNA Stool Mini Kit. Use forward primer GC-338F (5'-CGC CCG GGG CGC GCC CCG GGG CGG GGC GGG GGC GCG GGGGGC CTA CGG GAG GCA GCA G-3') and reverse primer 518R (5'-ATT ACC) in PCR reaction system GCG GCT GCT GG-3') amplifies the V3 region of bacterial 16S rDNA.

[0042] PCR amplification system and program are: 10×PCR buffer 5μL; dNTP (2.5mM) 3.2μL; rTaq (5U / μL) 0.4μL; GC-338F (20μM) 1μL; 518R (20μM) 1μL; template DNA 50ng; ddH 2 0 to 50 μL. Pre-denaturation at 94 °C for 5 min; denaturation at 94 °C for 1 min, renaturation at 55 °C for 45 s, extension at 72 °C for 1 min, 30 cycles; final extension at 72 °C for 10 min.

[0043] Denaturing gradient gel electrophoresis (...

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Abstract

The invention relates to a porphyra haitanensis polysaccharide as well as a preparation method and probiotic activity application thereof. Porphyra haitanensis is used as a raw material and is extracted through a traditional hot water extraction method; after the porphyra haitanensis is precipitated through ethanol, obtained polysaccharide sediment is re-dissolved into a low-concentration salt solution; after dialysis and freeze drying are carried out, the porphyra haitanensis polysaccharide with greatly improved solubility and viscosity is obtained; the effect of regulating intestinal flora of human bodies is easier to realize and the immunity is improved. The polysaccharide can be applied to preparation of medicines and / or foods with probiotic activity. The extracted porphyra haitanensispolysaccharide is added into yogurt to prepare yogurt chewable tablets; the obtained polysaccharide yogurt chewable tablets integrate effects of prebiotics and probiotic bacteria; the production costis low and the polysaccharide yogurt chewable tablets have no side effect on the human bodies; the polysaccharide yogurt chewable tablets have the effects of regulating the intestinal flora of the human bodies and enhancing the immunity of organisms, are convenient to carry and is convenient for oral administration.

Description

technical field [0001] The invention belongs to the field of health food, in particular to a laver polysaccharide, a preparation method thereof, and a probiotic activity application. Background technique [0002] Intestinal flora plays an important role in human health and is an important "microbial organ" of the human body. Studies have shown that intestinal flora can regulate intestinal motility and secretion, decompose macromolecular complex polysaccharides in food, participate in the digestion and absorption of nutrients, maintain the integrity of the intestinal epithelial barrier, and promote and maintain the normal development of the immune system. and activities, etc. Therefore, the gut microbiota regulates the normal physiological functions of the host and the development of diseases. At present, it has been clearly recognized that the physiological metabolism of the human body is not only controlled by its own genes, but also by the intestinal flora. Dietary poly...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23C9/18A23C9/13A23C9/123A61K31/715A61P1/00
CPCA23C9/123A23C9/1234A23C9/1238A23C9/1307A23C9/18A61K31/715A61P1/00C08B37/00C08B37/0003
Inventor 张杰良徐书映
Owner SHANTOU UNIV
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