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Porphyra metal-chelating protein peptide and preparation method thereof

A metal chelate protein, metal chelate peptide technology, applied in Fe, Zn) chelate peptide, metal (Ca field, can solve the problem of zinc absorption and metabolism limitation, zinc deficiency and so on

Active Publication Date: 2015-05-20
FUZHOU UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] Although zinc sources are very abundant in nature, the absorption and metabolism of zinc in the human body is restricted by various factors. Zinc deficiency has become a public health problem in my country and many developing countries.

Method used

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  • Porphyra metal-chelating protein peptide and preparation method thereof
  • Porphyra metal-chelating protein peptide and preparation method thereof

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Experimental program
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Embodiment 1

[0030] The preparation method is as follows:

[0031] Weigh 5.0 g of laver protein and dissolve it in 100 ml of distilled water, then adjust its pH to 7.0 with 2 mol / L NaOH. First heat the solution to 55°C in a water bath, then add the corresponding amount of enzyme according to the enzyme-substrate ratio of 1:25, the mass ratio of compound protease to flavor protease is 2:1, and the enzymatic hydrolysis time is 7 hours . Then inactivate the enzyme in a boiling water bath for 10 minutes, then centrifuge at 10,000 rpm for 10 minutes after cooling, and collect the supernatant for later use.

[0032] The supernatant was separated by TOYOPEARL DEAE-650M anion exchange chromatography (50 cm in length, 1.6 cm in outer diameter), and the eluent was 0.02 mol / L pH 9.0 phosphate buffer with concentration gradient 0-0.5 M NaCl, flow rate At 0.5 mL / min, samples from each peak were collected and assayed for metal (Ca, Fe, Zn) chelation activity.

[0033] The elution peak with the highes...

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Abstract

The invention provides porphyra metal-chelating protein peptide and a preparation method thereof. The amino acid sequence of peptide is eagdsqekv. The preparation method comprises the following steps: by taking porphyra protein as a raw material, compounding composite protease and flavourzyme for enzymolysis of porphyra protein, and performing separation purification and freeze drying, thereby obtaining metal-chelating protein. On the theoretical basis that polypeptide has action sites for chelating with metal ions and can form a stable compound with metal ions, and a polypeptide-metal chelate has unique chelating mechanisms and operation mechanisms, can be easily absorbed and can simultaneously supplement amino acid and metal, peptide with high metal (Ca, Fe and Zn) chelating activity can be prepared through cutting, and the metal-chelating activity can be efficiently brought into play.

Description

technical field [0001] The invention relates to a metal (Ca, Fe, Zn) chelating peptide, and more specifically relates to a metal chelating peptide prepared by enzymatically hydrolyzing laver protein with complex protease and flavor protease, belonging to the field of biotechnology. Background technique [0002] Porphyra protein-derived bioactive peptides have extremely high nutritional value, food safety and physiological health functions, and play an important role in promoting the growth, development and disease prevention of the body, and can be used as functional food or food additives and even medicines applied to people's daily life. [0003] Calcium deficiency is a global nutritional problem. Because our people mainly eat plant-based diets, the phenomenon of calcium deficiency is even more serious. Therefore, calcium supplementation has become an important topic in the study of dietary nutrition in our country. Traditional inorganic calcium salts, such as calcium car...

Claims

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Application Information

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IPC IPC(8): C07K7/06C12P21/06C07K1/20C07K1/18C07K1/16
Inventor 汪少芸林佳萍吴妙鸿付才力赵立娜
Owner FUZHOU UNIV
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