151 results about "Porphyra haitanensis" patented technology

The invention discloses an acidizing fluid for green algae and a disease infected cell in porphyra haitanensis cultivation and a treating method thereof. A solvent for the acidizing fluid is clean seawater, and solute for the acidizing fluid is at least one of hydrochloric acid and citric acid, wherein the concentration expressed in percentage by weight of the solute in the acidizing fluid is 0.3 to 1.5 percent. The green algae and the disease infected cell can be quickly killed by a treating method of preparing the acidizing fluid, shipping, dipping for 15 to 20 seconds and rinsing, killing effect is better, the killing rate is over 90 percent, and the influence on the growth of the porphyra haitanensis is less. The treating method has the advantages of no restriction of natural condition, labor intensity reduction and the like.

The invention relates to an indoor conchospore seedling picking method for porphyra haitanensis shell conchocelis. The seedling picking method comprises the following steps: (1) preparing 7 days before conchospore seedling picking; (2) preparing 1 day before conchospore seedling picking; (3) carrying out conchospore seedling picking. The method accords with the biological property of porphyra haitanensis, can meet the productive conchospore seedling picking requirement of porphyra haitanensis, is a technical method for large-scale cultivation and centralized seedling providing of porphyra haitanensis, overcomes the defects that plentiful shells are irritated while being conveyed to a sea area and operation in a far sea area is failed, and has the advantages that conchospore seedling picking and large-scale seedling providing of porphyra haitanensis can fulfilled indoors, thereby providing convenience for cultivation and centralized seedling providing of porphyra haitanensis.

The invention relates to an application of a seaweed polypeptide in preparing a health-care product for reducing blood fat and blood sugar as well as a beverage containing the polypeptide for reducing the blood fat and the blood sugar. The seaweed polypeptide is prepared by the following steps of: milling a mixture of immersed porphyra haitanensis and water through a colloid mill to obtain porphyra haitanensis slurry; adopting AS.1398 neutral protease to carry out enzymolysis on the porphyra haitanensis slurry for 5-10 hours under the conditions that the temperature is 45-55 DEG C, the pH (Potential of Hydrogen) is 7.2-7.8, the enzyme adding amount E/S is 8000-12000 U/g and the weight/volume ratio concentration of a substrate is 3-7%, so as to obtain an enzymolysis solution; centrifuging the enzymolysis solution and collecting liquid supernatant; and carrying out ultra-filtering separation on the liquid supernatant to obtain an active component with the molecular weight of less than 10000 Da. An animal experiment shows that agars are subjected to biological enzymolysis to obtain the seaweed polypeptide which has the obvious effects of reducing the blood fat, the blood sugar and blood pressure; and after the seaweed polypeptide is taken for a long period, the weight can be prevented from too fast increase and the happening possibility of arteriosclerosis can be reduced.

The invention provides a preparation method and application of a low-molecular porphyra haitanensis polyferose compound. The method is characterized in that low-molecular porphyran is used as a raw material and degraded by redox reaction to obtain low-molecular-weight porphyran with the molecular weight being less than 10000Da; and the polyferose compound synthesized by heating the porphyran and ferric chloride together can be dissolved well and exist stably under a physiological pH condition, and also shows a good effect in the iron-deficiency anemia mouse model experiment. The preparation method has the advantages of being simple and convenient in operation, stable in preparation process, low in preparation cost, etc.; and the preparation method also shows wide applicability to raw materials, namely, any polysaccharide with a proper molecular weight range is applicable to the preparation method.

The utility model relates to a reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis, belonging to fingerprint chromatogram of porphyra haitaensis, in particular to reconstruction method and application for identifying fingerprint chromatogram of porphyra haitaensis. The utility model provides a rapid accurate reconstruction method for identifying fingerprint chromatogram of porphyra haitaensis and the application for realizing the porphyra haitaensis identification automation, which is characterized in that porphyra haitaensis specimens are collected; genome DNA of various specimens is refined and purified; the PCR is enlarged, the refined genome DNA is utilized as a template, SRAP molecule marking analysis is performed by the optimized experimental system; the representative SRAP multi-state bars are selected for reconstructing the DNA fingerprint chromatogram of various specimens; the reconstructed DNA fingerprint chromatogram is converted to the identifiable digital fingerprint by computer, and then is inputted to the fingerprint chromatogram identification software, thereby the digital fingerprint database of porphyra haitaensis is established. The utility model is used for follow-up porphyra haitaensis automation identification.

The invention relates to a method for identifying porphyra haitanensis in different harvesting periods. The method comprises the steps of pretreatment, smashing, centrifugation, suction filtration, vacuum freeze drying, nuclear magnetism spectrum collection and data analysis and identification. The method specifically comprises the following steps: performing Fourier transformation on all one-dimensional 1H-NMR spectrum data of a sample to be identified to obtain a spectrogram, then performing signal assignment, phase adjustment, base line correction, calibration and integration, and performing multi-variable data analysis on the normalized NMR data by using SIMCA-P+ software; performing main component analysis on the NMR data, performing orthogonal partial least squares discriminant analysis on the NMR data, and taking the automatically normalized NMR data as an X variable and grouping information as a Y variable. PCA analysis results of the NMR data of extracts of the porphyra haitanensis in different harvesting periods are obviously different, and nori harvested in the previous period and nori harvested in the middle period are mainly distinguished in PC2; however, nori harvested in the later period is mainly different from the nori harvested in the previous period and the nori harvested in the middle period in PC1.

A molecular marker for discriminating the excellent variety of ampullaceous laver is disclosed. Its application includes using AFLP marker technique to analyze the excellent varieties of ampullaceous laver, finding out the AFLP marker specific to excellent variety, converting it to SCAR marker, designing specific primer Marker and Marker 2, preparing reagent kit, PCR amplifying to individual, and discriminating. It can be also be used for marker said seed culture.

The invention discloses a diffusing method for conchospore of porphyra haitanensis, comprising the following steps of: firstly, mounting a water pump in a seedling collecting pool in advance, arranging a mature shell of a sporosac branch at the bottom of the pool, and paving multiple layers of nylon rope nettings in the water above the shell; secondly, pumping water in the seedling collecting pool by using the water pump to flow the water, and returning the water pumped by the water pump so that the water in the whole seedling collecting pool flows; thirdly, blowing the side from the bottom of the pool upwards by using a blast blower through an aerating pipe with a wall hole so that the water in the seedling collecting pool is surged to stir the water, the shell and the nylon rope nettings of the whole seedling collecting pool, and impacting the shell by the water flow, wherein the conchospore on the sporosac branch of the shell is much active through stimulation, and the conchospore is diffused from the sporosac branch of the shell to be attached on the nylon rope nettings. The method has good diffusing effect, high diffusing efficiency and high labor efficiency; moreover, the method saves time and labor.

The invention belongs to the technical field of polysaccharide derivatization of the marine drug field, in particular relates to a porphyra haitanensis polysaccharide positioning sulphating method which comprises steps of totally sulfating porphyra haitanensis polysaccharide, preparing 6-O-sulfated porphyra haitanensis polysaccharide, preparing 2,2',4-O-sulfated porphyra haitanensis polysaccharide, and the like. High molecular weight polysaccharide obtained from water extract and alcohol precipitate is used as a raw material, hydroxyl at a specific part of the polysaccharide is protected, and subsequently sulphating derivatives of different parts are synthesized. A modified product provided by the invention is good in solubility, has different anticoagulation effects in in-vitro anticoagulation experiment, and is applicable to different medical demands.

The invention provides a method and device for cultivating porphyra haitanensis and laminaria japonica alternately in a full-floating mode in the shallow sea. The method includes the first step of seedling collection and cultivation of the porphyra haitanensis and the second step of cultivation of the laminaria japonica. According to the method, the period of nearly a mouth for temporary seedling cultivation before efficient cultivation of the porphyra haitanensis and the laminaria japonica is fully utilized and serves as a grafting buffer period, and cooperation is achieved; laminaria japonica seedlings start to be cultivated on only 10% of a seedling growing raft frame 15 days before harvest of the porphyra haitanensis is finished, the seedlings are clamped when the length ranges from 10 cm to 15 cm and are placed on a cultivation raft frame step by step till the seedlings are spread on the whole cultivation raft frame, cultivation of the laminaria japonica is completed when June comes, and in this way, about 20 days to 30 days of adjustment time can be reserved; five-to-ten-layer temporary cultivation is generally adopted in seedling emergence of the porphyra haitanensis, seedlings can emerge 20 days to 30 days later and are placed on the cultivation raft frame step by step till the seedlings are spread on the whole raft frame, and in this way, about 20 days to 30 days of adjustment time can be reserved as well. The possible problem of time conflict in alternate cultivation of the porphyra haitanensis and the laminaria japonica is solved by setting the buffer period.

The invention relates to a recombined lipoxygenase PhLOX and a preparation method thereof. The PhLOX has activities of a lipoxygenase, a hydroperoxide lyase and a propadiene oxide synthetase at the same time. The method includes following steps: extracting total RNA from porphyra haitanensis; designing a primer according to a gene sequence of the lipoxygenase; amplifying an overall-length cDNA sequence through an RACE technology; recombining the overall-length cDNA sequence to an expression vector pET-28a; converting escherichia coli E.coli BL21; screening out positive clone; and generating the multifunctional lipoxygenase of a bacteria liquid with an expression quantity being 2mg/L through inducible expression. Compared with a lipoxygenase in the prior art, the recombined lipoxygenase has a plurality of enzyme functions, is free of a strict substrate specifity and allows downstream products in various structures to be formed.

The invention discloses an inserted-bar type synchronous dry-out method for porphyra haitanensis. Suspension rings are added to the places where stay bars, inserted bars and lifting ropes are connected, and the lifting ropes can slide freely relative to the stay bars and the inserted bars, so that a linkage type sliding device is formed; when observed from the side face, each lifting rope is in vertical serial connection between the corresponding inserted bars and the corresponding stay bars and can slide freely, therefore when the web position height is adjusted, adjustment of the height of each whole row of web curtain can be completed by only retracting or extending one end of the corresponding lifting rope, and particularly when the lifting ropes are operated during rising tide, simpleness is achieved, and labor intensity is low; due to the fact that a whole raft rack is consistent in material, growth of porphyra haitanensis is basically synchronous, from the mechanical angle, the suspension rings of the identical inserted bars and stay bars are the same in stress, only the lifting rope at one end is tightened or loosened, time control over synchronous water-in and dry-out can be achieved, the dry-out and water-in time can be controlled artificially, and synchronism is good; by means of a device adopted according to the method, the structure of culture facility is slightly changed, investment is small, cultured laver is good in quality and high in output, and production benefits are remarkably increased.

The invention discloses a reciprocating swing type porphyra haitanensis cleaning device which comprises a cleaning box. A top plate is supported and fixed at the top of the cleaning box through multiple supporting rods, a supporting shaft is rotatably arranged in a middle rectangular groove of the top plate, a stirring rod is fixedly arranged on the supporting shaft, a second motor connected with the supporting shaft is also installed on the rear side of the top plate, a U-shaped frame is installed at the lower end of the stirring rod, a carrier screen cylinder is installed at the lower portion of the U-shaped frame, a guiding frame is installed at the top of the top plate, a guide plate is arranged at the lower portion of the inner side of the cleaning box, a sedimentation cylinder is installed in the middle of the guide plate, a first motor is installed in the middle of the bottom of the cleaning box, spiral blades are installed on the inner side, on the output shaft of the first motor, of the sedimentation cylinder, and a filter screen cylinder is arranged on an outer ring of the lower side of the guide plate. The reciprocating swing type porphyra haitanensis cleaning device achieves quick porphyra haitanensis cleaning, makes cleaning thorough and saves water resources.

The present invention discloses a trans-regional relay seedling method for porphyra haitanensis. The method comprises: inoculating protonema or carposporophyte of porphyra haitanensis on shell strings in southern part of Zhejiang and sea area of Fujian; after the protonema of shells is cultivated to conchosporangium by adopting a three-dimensional seedling method, promoting maturity by way of shortening the illuminating time, reducing the illuminating intensity and adjusting the adding proportion of a nutritive salt; and transporting mature shells with sporangia to the north of Jiangsu, releasing conchospore by virtue of the thermal expansion principle, and culturing the conchospores attached to a net cord into a sea area. The seedling method disclosed by the present invention has the advantages that shell protonema is cultured rapidly in the south, and is transported to the north if being mature to perform spore dispersion and seedling collection for the first time. The seedling method disclosed by the present invention is suitable for large-scaled porphyra haitanensis cultivation in the north in advance, or performing efficient cultivation by transporting the seedling nets back to the south. Furthermore, the collected seedling shells can be transported back to the south for continuous secondary cultivation, secondary seedling collection is performed in shells in the same batch and are cultured in the south. The method is suitable for being widely applied in porphyra haitanensis seedling fields.

The invention belongs to the technical field of porphyra haitanensis molecular breeding and specifically provides a method for establishing a cross breeding system with the use of porphyra haitanensis molecular marker-assisted selection. The method can quickly and effectively select strains of breed with excellent properties, shorten breeding process and provide a new breeding system for the development of dried purple seaweed breeding.

The present invention relates to algae cultivating technology, and is especially the method of cultivating Porphyra haitanensis and Porphyra yezoensis alternately in the laver cultivating area. The method can raise the utilization of cultivation area and cultivation apparatus and raise production.

The invention discloses rice flour for preventing Alzheimer's disease. The rice flour is prepared from the following components in parts by weight: 10 to 15 parts of food composition, 60 to 80 parts of rice meal, 12 to 18 parts of malt flour, 10 to 15 parts of skim milk powder, 5 to 8 parts of multivitamin and 5 to 10 parts of a natural sweetener, wherein the food composition is prepared from the following components in parts by weight: 10 to 20 parts of garlic extract, 5 to 15 parts of porphyra haitanesis extract and 2 to 8 parts of radix puerariae extract; the rice flour is prepared by mixing the components and then carrying out nanometer crushing treatment on the mixture. The rice flour disclosed by the invention is formed by scientific and reasonable matching and has the advantages of reasonable proportion, high pertinence, good mouth feel, convenience in eating, easiness in digestion and absorption, good instant solubility and good reconstituability; in addition, the rice flour has the efficacy of delaying senility effectively, preventing the Alzheimer's disease and the like, and is quite suitable for middle-aged and elderly people to eat.

The invention discloses a method for removing chromium off porphyra haitanensis. The method is implemented by putting fresh porphyra haitanensis and de-ionized water in weight volume ratio of 4.5-5.5:25 into a container, moving the container into an ultrasonic extractor, and performing ultrasonic treatment to the porphyra haitanensis in the container in the interval ultrasonic way of using ultrasonic wave for o.3-0.6 seconds and pausing for 0.7-0.4 seconds with ultrasonic wave temperature being 25-45 DEG C, ultrasonic wave frequency being 25kHz and total ultrasonic times being 10-20 minutes; after the ultrasonic treatment with the above way and conditions, the removal rate of chromium off porphyra haitanensis can reach above 75%, the residual quantity of chromium in porphyra haitanensis meets the requirement of national standard basically, thus reducing harm to human bodies of people and especially children after eating, not causing contamination to porphyra haitanensis, having littleinfluence on quality of porphyra haitanensis, less removal time, high speed and high removal rate.

The invention provides a method for preventing and controlling green alga overgrowth on porphyra cultivation raft frames and relates to the application of coatings such as water tank paint on the prevention and control over green alga overgrowth on the porphyra cultivation raft frames. The method comprises the steps that green alga overgrowth prevention paint is smeared on the porphyra cultivation raft frames, and the green alga overgrowth prevention paint is finishing paint, shipbottom paint, the water tank paint and the like. The method for preventing and controlling the green alga overgrowth on the porphyra cultivation raft frames can be used for preventing and controlling the green alga overgrowth on the cultivation raft frames in the cultivation industries of porphyra yezoensis and porphyra haitanensis.

The invention discloses a method for removing arsenic in Porphyra haitanensis. Fresh Porphyra haitanensis and deionized water are put into a container by weight-to-volume ratio of 4.5-5.5:25. The container is moved into an ultrasonic extractor. Ultrasonic treatment is conducted to the Porphyra haitanensis in the container under ultrasonic temperature of 25-45 DEG C and ultrasonic frequency of 25 KHz in a way that ultrasonic waves are emitted for 0.3-0.6 seconds at intervals of 0.7-0.4 seconds. The total time of the ultrasonic treatment is 10-25 minutes. Through the ultrasonic treatment under the conditions and in the way, the removal rate of the arsenic in Porphyra haitanensis can reach more than 60 percent, the residual arsenic in Porphyra haitanensis is lower than the value required by the national standard, and the harmfulness to the bodies of people, especially the bodies of children, is reduced. The method has the advantages that no pollution is caused to Porphyra haitanensis, the impact on the quality of Porphyra haitanensis is small, the removal time is short, the speed is fast and the removal rate is high.

The invention relates to a fresh keeping method of porphyra haitanensis. The fresh keeping method sequentially comprises the following steps: pretreatment, bacterium reduction treatment, fresh keeping treatment, centrifugal dewatering, vacuum packaging and preservation, wherein the pretreatment concretely comprises the following steps: washing fresh porphyra haitanensis by clear seawater for at least one time so as to remove sediments in the porphyra haitanensis, and introducing air into the clear seawater by using aeration equipment to generate bubbles so as to turn over the porphyra haitanensis in the process of washing the porphyra haitanensis by the clear seawater. According to the fresh keeping method of the porphyra haitanensis disclosed by the invention, as air introducing and bubbling in the clear seawater are capable of fully turning over the porphyra haitanensis and impacting the surface of the porphyra haitanensis, the sediments are completely washed, sediment removal speed is higher than that of ordinary washing, and sediment removal effect is good; along with sediment removal, total content of microorganisms can also be lowered to maximum, thus greatly lowering difficulty of subsequent treatment of the porphyra haitanensis; meanwhile, the structure of the porphyra haitanensis can not be damaged by turning over the porphyra haitanensis by utilizing an air flow in the aeration equipment, so that shape of the porphyra haitanensis can be well kept.