40 results about "Residual vol" patented technology

100-stage cleaning laminar flow inoculation under barotropic atmosphere during edible fungus industrial cultivation belongs to edible fungus production technology. The invention aims to solve the following problems: the existing open inoculation is inadvisable to be adopted under the conditions of plum rain seasons and high temperature and high humidity climate; poisonous residual quantity emerges when spatial standard sterilization is carried out in the inoculation environment; and the success rate of inoculation generally fluctuates from 97% to 99%. The invention is characterized in that fresh air enters into the top of the workshop under the pressure higher than the normal atmosphere pressure and is exhausted downwards after being efficiently filtered and purified by a 100-stage cleaning laminar flow cover; the inoculators, the inoculation machines and the fungus flask transfer cars are all arranged in 100-stage cleaning laminar flow from top to bottom; and the barotropic atmosphere of the 100-stage cleaning laminar flow from top to bottom is at least higher than 30Pa, the gas pressure in the cooling workshop and the corridor of the fungus culturing workshop. The invention preliminarily shows the following beneficial effects: inoculation can be carried out in due time at all seasons according to the production requirements; the problem of poisonous residual quantity emerging after standard sterilization is prevented; and the success rate of inoculation is stably as high as 99.7-99.9%.

The invention discloses fresh milk and a preparation technology thereof. The preparation technology comprises the following steps: filtering raw milk to remove impurities such as dust in the raw milk;centrifuging the filtered raw milk to remove sundries such as non-laticiferous cells in the raw milk; performing reverse osmosis membrane concentration, milk fat separation, degassing and sterilization on the purified material, wherein low-temperature standarization of the purified material is realized through the reverse osmosis membrane concentration. Meanwhile, through cooperation of the stepsand order, bacteria in the raw milk are killed, and the nutrition loss of the fresh milk is low; according to tests, total residue of microorganisms in the fresh milk is less than or equal to 1 cfu/mL, including less than 1 cfu/mL of total spores and less than 1 cfu/mL of total bacterial colony; with respect to the nutritive indexes, the content of furosine is less than or equal to 12 mg/100g, the content of beta-lactoglobulin is greater than or equal to 2,400 mg/L, the alkaline phosphatase is negative, and the content of lactoferrin is greater than or equal to 25 mg/L.

The invention discloses a method for removing chromium off porphyra haitanensis. The method is implemented by putting fresh porphyra haitanensis and de-ionized water in weight volume ratio of 4.5-5.5:25 into a container, moving the container into an ultrasonic extractor, and performing ultrasonic treatment to the porphyra haitanensis in the container in the interval ultrasonic way of using ultrasonic wave for o.3-0.6 seconds and pausing for 0.7-0.4 seconds with ultrasonic wave temperature being 25-45 DEG C, ultrasonic wave frequency being 25kHz and total ultrasonic times being 10-20 minutes; after the ultrasonic treatment with the above way and conditions, the removal rate of chromium off porphyra haitanensis can reach above 75%, the residual quantity of chromium in porphyra haitanensis meets the requirement of national standard basically, thus reducing harm to human bodies of people and especially children after eating, not causing contamination to porphyra haitanensis, having littleinfluence on quality of porphyra haitanensis, less removal time, high speed and high removal rate.

The invention discloses a method for removing arsenic in Porphyra haitanensis. Fresh Porphyra haitanensis and deionized water are put into a container by weight-to-volume ratio of 4.5-5.5:25. The container is moved into an ultrasonic extractor. Ultrasonic treatment is conducted to the Porphyra haitanensis in the container under ultrasonic temperature of 25-45 DEG C and ultrasonic frequency of 25 KHz in a way that ultrasonic waves are emitted for 0.3-0.6 seconds at intervals of 0.7-0.4 seconds. The total time of the ultrasonic treatment is 10-25 minutes. Through the ultrasonic treatment under the conditions and in the way, the removal rate of the arsenic in Porphyra haitanensis can reach more than 60 percent, the residual arsenic in Porphyra haitanensis is lower than the value required by the national standard, and the harmfulness to the bodies of people, especially the bodies of children, is reduced. The method has the advantages that no pollution is caused to Porphyra haitanensis, the impact on the quality of Porphyra haitanensis is small, the removal time is short, the speed is fast and the removal rate is high.

The present invention discloses a method for determining the amount of residual dithiocarbamate bactericide in grain through a gas chromatography method. The determination method comprises that: a sample reacts with a reducing acid solution in a closed container, dithiocarbamate is decomposed, carbon disulfide is quantitatively released and is absorbed with an organic solvent, the organic layer is taken and is subjected to chromatography analysis by using a gas chromatograph (with FPD-S filter), qualitation is performed on the target substance according to the chromatography peak retention time, and quantitation is performed through an external standard method. According to the present invention, the organic solvent is adopted to absorb the carbon disulfide generated from acidification decomposition of the dithiocarbamate bactericide, and the analysis through the gas chromatograph is performed, such that the instability of the headspace sample injection is overcome, the damage of the acid condition on the column is overcome, the interference can further be eliminated, and the qualitative and quantitative analysis can be well performed; and the minimum detection limit of the method is 0.04 mg/kg.

The invention provides a high-performance liquid chromatography for detecting the residual amount of matrine in tobacco. The high-performance liquid chromatography comprises the following steps that (1) preparing a standard matrine solution, including weighing a standard matrine of a certain mass, dissolving with methanol, titrating to obtain a standard stock matrine solution, and diluting the standard stock matrine solution stepwise to obtain standard work solutions with a series of concentration gradients; (2) extracting, including extracting the matrine in tobacco by using dichloromethane as an extracting agent; (3) purifying, including purifying an extract by using an alumina SPE (solid phase extraction) columella; (4) detecting by using the liquid chromatography; (5) establishing a standard curve; and (6) computing and analyzing the matrine residue. The high-performance liquid chromatography provided by the invention is characterized by being simple, accurate, rapid and reliable and is of great importance to controlling the residual amount of matrine in tobacco and guiding the safe and reasonable application of matrine to tobacoo.

The present invention discloses a sulfur dioxide controlled atmosphere preservation method of grapes. The method comprises the following steps: (1) whole ripen grapes without any mechanical injury anddisease and pest injury are placed in a single layer in a plastic basket with holes in five sides, and the grapes are put into a controlled atmosphere cold storage; (2) the grapes are precooled at -1to 1 DEG C for 24-30 h and 1-methylcyclopropene gas is used in a whole precooling period to conduct a sealed fumigation treatment; and (3) the treated grapes in the step (2) are preserved at a condition of -1 to 1 DEG C, a relative humidity of 85-90%, a SO2 volume concentration of 0.001-0.003%, an O2 volume concentration of 2-6%, a CO2 volume concentration of 2-8% and the balance of N2. The technology is used to preserve the grapes, can effectively prevent browning, dry stems and grain falling of the grape fruits, preserves quality of the fruits, extends a storage period, has a preservation period extended for 10-20 days compared with a common cold storage treatment, and is low in sulfur dioxide residual amount and high in quality.

The invention establishes an analytical method for measuring the residual amounts of estradiol, estriol and oestrone in cosmetics by highly efficient liquid chromatography-tandem mass spectrometry. The method comprises the following steps: with methanol as an extraction solvent, ultrasonically extracting different kinds of cosmetic samples for 30min; centrifuging the sample extracting solution with a speed of 12000r/min for 20min; with acetonitrile and water (in a volume ratio of 40:60) as a mobile phase, performing constant gradient elution, wherein the flow speed is 0.2ml/min and the column temperature is 25 DEG; and performing separation by an EclipseXDB-C18 (with the area of 2.1mm*150mm and the grain size of 5 microns) chromatographic column. The result indicates that the correlation coefficient R of an equation of linear regression of the three hormones is in the range of 0.9983-0.9998, the recycling rate is in the range of 95.21%-106.19% and the accuracy degree is in the range of 1.70%-4.82%. The method for measuring the residual amounts of hormones in cosmetics is simple, agile, rapid, reliable, good in reproducibility and applicable to analysis and detection on the content of hormones in cosmetics.

A detecting method for trichlorfon enantiomers in culture pond bottom mud includes the steps that a bottom mud sample is placed into a centrifugal tube, anhydrous sodium sulfate is added to remove water, acetic acid is added to acidize acetonitrile, centrifuging is performed after ultrasonic extraction is performed, an upper layer extracting solution is taken into a flask, the extracting process is repeated 2-3 times, extracting solutions are mixed, the mixed solution is subjected to rotary evaporation and concentration in a water bath at 27 DEG C, a concentrated solution is added into CARB/NH2 cartridges activated in advance, the acetonitrile is used for flushing the flask, flushing liquid passes through the CARB/NH2 cartridges, outflow liquid is collected, and the CARB/NH2 cartridges are blown through nitrogen to be almost dry, hexyl hydride/isopropanol is used for performing redissolving and volume constancy on the liquid to 1 mL, and then the liquid passes through a microporous filtering film of 0.22 micrometer to obtain a solution A; with the hexyl hydride/isopropanol being a moving phase, separation is performed on the trichlorfon enantiomers; a high-performance liquid chromatograph is used for detecting the solution A, (as shown in specifications) IC chiral columns are selected for usage, the temperature of the columns is 25 DEG C, the flow rate is 1 mL/min, and the wavelength is 207 nm. The trichlorfon chiral enantimoers can be successfully separated, in this way, the residual quantity of the trichlorfon enantiomers in the bottom mud is detected, and bases are provided for residual risk estimation and reasonable and safe using of trichlorfon in bottom mud culture.

The invention discloses a method for measuring the residual quantity of a pesticide adjuvant based on gas chromatography, and particularly relates to the field of detection of the residual quantity ofthe pesticide adjuvant, wherein the detection steps are as follows: S1, preparing a sample; S2, carrying out sample extraction; S3, carrying out sample purification; S4, preparing in the early stage of measurement; S5, measuring; S6, carrying out parallel test; S7, carrying out blank test; and S8, analyzing result and calculating. According to the invention, a sample is homogenized and extracted by acetonitrile, and subjected to salting-out centrifugation, a supernatant is taken, the sample is purified by PSA, the sample is detected by a gas chromatography-mass spectrometer, a characteristic selection ion monitoring scanning mode (SIM) is adopted, the qualitative analysis is carried out according to the retention time and the abundance ratio of qualitative ion fragments, and the quantification is carried out by an external standard method, so that the measurement of the residual quantity of the pesticide adjuvant is more accurate, and compared with the traditional gas chromatography, the detection precision is higher and the detection is more efficient.

The invention relates to a method for refining liquid hydrocarbon. In the method, a fixed-bed adsorber is adopted, active carbon and granular clay are used as an adsorbent, the liquid hydrocarbon in the adsorber sequentially passes through an active carbon bed and a granular clay bed of an adsorbent bed, so that oil stains, moisture and other impurities in the liquid hydrocarbon are adsorbed and separated, wherein the adsorption temperature is between 25 below zero and 52 DEG C, the adsorption pressure is between 0.1 and 2.5MPa, and the weight space velocity is between 0.5 and 20h<-1>; the filling height ratio of the granular clay to the active carbon is 0.1 to 5; and the height-diameter ratio of the adsorbent is 3 to 30. By using the method for refining the liquid hydrocarbon, the moisture, oil stains and other impurities in the liquid hydrocarbon can be effectively removed; the problems of evaporation residual quantity and unqualified oil stains of the liquid hydrocarbon are radically solved; and the method has the advantages of simple process, low cost, reproducibility and reusability of the active carbon and the granular clay, no discharge of wastewater and waste alkali liquor, and environmental protection due to the adoption of the fixed-bed adsorber and the active carbon and granular clay composite adsorbent.

The invention discloses a method for simultaneously determining six pyrethroid pesticide residues in poultry eggs. The method comprises the following steps that S1, a sample to be tested is weighed, dispersion extraction is performed by QuEChERs, after supernatant is filtered, the sample to be tested is obtained, and a matrix standard curve is drawn; S2, the sample to be tested obtained in the step S1 is simultaneously subjected to determination of the residual amount of six pyrethroid pesticides by liquid chromatography tandem mass spectrometry, and retention time and ion fragment abundanceratio are used for qualitative analysis, and according to the area of an ion peak, an external standard method is used for quantitative analysis; the poultry egg samples are subjected to QuEChERS purification, the matrix interfering the measurement in the samples is effectively removed, blank sample matrix calibration is performed, the six pyrethroids pesticide residues are determined simultaneously by using liquid chromatography tandem triple quadrupole, using retention time and ion fragment abundance ratio for qualitative analysis and using an external standard method for quantitative analysis according to the area of the ion peak. After optimization, the recovery rate of the target pesticide compound in eggs reaches 77.1%-109.4%, the precision and stability test RSD within 24h is less than or equal to 15%, and the linear range is within 0.005mg/kg-0.200mg/kg.

The invention discloses a GC-NCI-MS (gas chromatography-negative chemical ionization-mass spectrometry) determination method of residual amount of tetrachlorantraniliprole. The method is mainly used for determining the residual amount of tetrachlorantraniliprole in cereal grains, animal-derived foods and other complex matrix food agricultural products. The method comprises the following steps: homogenizing and extracting residual tetrachlorantraniliprole in a sample with acetonitrile or an acetonitrile solution containing 1% of acetic acid, performing purification and concentration by a C18/PSA solid-phase extraction column, performing GC-NCI-MS detection, adopting a blank matrix solution without a pesticide to be detected to establish a standard curve for correction, and quantifying by an external standard method. According to the method, the average recovery rate is 83.3%-90.3%, the average relative standard deviation (RSD) is 3.5%-6.9%, and the detection limit is lower than 1.43mu g/kg; and the method has the advantages of simplicity in operation, high speed, good impurity removal effect, high sensitivity, strong characteristic feature, good repeatability and accurate quantitative and qualitative properties. According to the method, the technical requirement of 'being standard without exceptions' of the residual limit of 0.01mg/kg can be met, and the powerful technical support is provided for ensuring food safety of people in China and health development of export trades.

The invention discloses a GC-EI-MS (Gas Chromatography-Electron Ionization-Mass Spectrum) detecting method for the residual quantity of spirotetramat. The GC-EI-MS detecting method is mainly used for detecting the content of residual spirotetramat in complex-matrix foods and agricultural products such as cereal grains and animal-derived foods. The GC-EI-MS detecting method comprises the steps of homogenizing by using acetonitrile or an acetonitrile liquid containing 1% acetic acid to extract residual spirotetramat in a sample; purifying and concentrating by using a C18/PSA solid-phase extraction column; then, carrying out GC-EI-MS detection; establishing a corrected standard curve by using a blank matrix liquid without a pesticide to be detected; and quantifying by using an external standard method. By using the GC-EI-MS detecting method, the average recovery rate is 81.4-89.3%, the average relative standard deviation (RSD) is 4.6-8.9%, and the detection limit is lower than 3.57mu g/kg; and the GC-EI-MS detecting method is simple and convenient to operate, rapid, good in impurity removal effect, high in sensitivity, good in repeatability and accurate in qualitation and quantitation. According to the GC-EI-MS detecting method, the technical requirements of America, European union, Japan and other countries on residual quantity limit in security detection of corresponding foods can be met, and the powerful technical support can be provided for ensuring Chinese food safety and the health development of foreign export trade.

The invention discloses a co-detection determination method of residual quantity of a preservative and an antioxidant in a water-based adhesive. The method of the invention comprises steps as follows: preparation of a sample solution, high performance liquid chromatography, calculation of determination results and the like. The co-detection determination method has advantages of short detection time, simple operation, high sensitivity, high recovery rate, good repeatability and the like. Under chromatographic conditions in the method, separation of chromatographic peaks of MI, CMI, BIT and BHT from chromatographic peaks of impurities is good, and correlation is good. Limits of detection are respectively 0.065, 0.121, 0.046 and 0.052 microgram/mL. The average recovery of standard addition at low-medium-high three addition levels is within 88.7-93.8%. The average relative standard deviation of sample determination results is less than 5%. It shows that the method has high recovery rate and good repeatability. The invention provides scientific basis for quality surveillance of the water-based adhesive.

The invention discloses an LC-MS-MS (liquid chromatogram tandem mass spectrum) detecting method for residual quantity of mandipropamid in vegetables and dried fruits and belongs to the technical field of pesticide residual quantity detection.The method comprises the steps that an acetonitrile solution is used for extracting mandipropamid remained in a sample in a homogeneity mode, LC-MS-MS detecting is conducted after the extracting solution is dispersed and purified, a correction standard curve is established through a blank matrix solution not containing pesticide to be detected, and quantitation is conducted through an external standard method.According to the method, the average recovery rate is 85.0-94.6%, the relative standard deviation (RSD) is 0.86-2.89%, the detection limit is 0.003 mg/kg, and the quantitative detection limit is 0.01 mg/kg.The method has the advantages that operation is easy, convenient and quick, sensitivity is high, repeatability is good, and quality and quantitation are accurate.The uniform limit technical requirement for the 0.01 mg/kg residual limit can be met, and strong technical support is provided for guaranteeing people food safety in China and health development of export abroad trades.

The invention discloses a GC-NCI-MS method for determining the residual amount of flufenapyroxamide, which is mainly used for measuring the residual flufenapyroxystrobale content in complex matrix food and agricultural products such as grains and animal-derived foods. Use acetonitrile or an acetonitrile solution containing 1% acetic acid to homogeneously extract the residual flufenapyramide in the sample, C ₁₈ After purification and concentration of the /PSA solid phase extraction column, gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) detection, a calibration standard working curve is established using a blank matrix solution that does not contain the pesticide to be tested, and the external standard method is used for quantification. The average recovery rate of this method is 88.9%-104.7%, the average relative standard deviation (RSD) is 4.2%-7.5%, and the detection limit is lower than 1.09 μg/kg. Strong characteristics, good repeatability, and accurate qualitative and quantitative advantages. It can meet the technical requirements of the United States, the European Union, Japan and other countries for corresponding product safety testing, and provide strong technical support for ensuring the food safety of our people and the healthy development of foreign export trade.

The invention discloses a GC (Gas Chromatograph)-EI (Electronic Impact Ionization)-MS (Mass Spectrometry) determining method of the residual quantity of fluxapyroxad. The GC-EI-MS determining method is mainly used for determining the content of residual fluxapyroxad in complex-matrix food and agricultural products of cereal grains, animal derived food and the like. The GC-EI-MS determining method comprises the following steps: homogeneously extracting the residual fluxapyroxad in a sample by using acetonitrile or an acetonitrile solution containing 1 percent of acetic acid; carrying out GC-EI-MS detection after purifying and concentrating by using a C18/PSA (Primary Secondary Amine) solid-phase extraction column; building a corrected standard work curve by adopting a blank-matrix solution which does not contain to-be-detected pesticide; quantifying through an external standard method. According to the GC-EI-MS determining method of the residual quantity of the fluxapyroxad, disclosed by the invention, the average recovery rate is 84.6 to 103.1 percent, the average RSD (Relative Standard Deviation) is 3.2 to 8.4 percent, and the detection limit is lower than 1.01 mug/kg; the GC-EI-MS determining method has the advantages that the operation is convenient and fast, the impurity removing effect is good, the sensitivity is high, the characteristic performance is strong, the repeatability is good, and qualitativeness and quantitativeness are precise; the technical requirements on safety detection of corresponding products of countries of America, EU (European Union), Japan and the like can be met, and a powerful technical support is provided for ensuring the safety of food for people in China and the healthy development of export trade.