GC-NCI-MS (gas chromatography-negative chemical ionization-mass spectrometry) determination method of residual amount of tetrachlorantraniliprole
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of GC-NCI-MS and tetrachlorantraniliprole is applied in the field of GC-NCI-MS determination of tetrachlorantraniliprole residues, and achieves the effects of simple operation, high sensitivity and avoiding matrix interference.
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0005] So far, there are no domestic and foreign reports on the detection method of tetrachlorantraniliprole residues in food and agricultural products. Gas chromatography-mass spectrometry (GC-NCI-MS) equipped with a negative chemical ionization source has great advantages in the analysis of pesticide residues in food and agricultural products.
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0033] Example 1: Detection of Tetrachlorantraniliprole residues in rice
[0034] (1) Sample pretreatment
[0035] extract
[0036] Weigh 5g of rice sample that has been thoroughly mixed into a 50mL centrifuge tube, add 5mL of water to mix, let it stand for 30min, add 20mL of acetonitrile accurately, extract homogeneously for 2min, add 3g of anhydrous magnesium sulfate and 2g of sodium chloride, and vortex for 1min , 7000r / min centrifugal 5min. After centrifugation, take 8 mL of the acetonitrile extract at 40°C for rotary steaming or nitrogen blowing to about 1 mL for purification.
[0037] purify
[0038] Pre-wash C with 5 mL of acetonitrile 18 / PSA solid phase extraction column, when the liquid level reaches the top of the adsorbent, transfer the above extraction solution into the column, wash the test tube with 2mL acetonitrile, and transfer the washing liquid into the SPE column, when the solution reaches the top of the adsorbent, add Add 4mL of acetonitrile to the col...
Embodiment 2
[0064] Example 2: Detection of Tetrachlorantraniliprole residues in pork
[0065] (1) Sample pretreatment
[0066] extract
[0067] Weigh 5g of pork sample (ground into flour) that has been thoroughly mixed into a 50mL centrifuge tube, add 20mL of water to resuscitate for 30min, then accurately add 20mL of acetonitrile solution containing 1% acetic acid, shake and extract for 20min, ultrasonically extract for 5min, add 3g of anhydrous Magnesium sulfate and 2g sodium acetate, after vortexing for 1min, centrifuge at 7000r / min for 5min. After centrifugation, take 8 mL of acetonitrile extract and rotate it at 40 °C or blow it with nitrogen until it is nearly dry, add 1 mL of acetonitrile and vortex it, and wait for purification.
[0068] purify
[0069] Pre-wash C with 5 mL of acetonitrile 18 / PSA solid phase extraction column, when the liquid level reaches the top of the adsorbent, transfer the above extraction solution into the column, wash the test tube with 2mL acetonitril...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Property
Measurement
Unit
Column length
aaaaa
aaaaa
The inside diameter of
aaaaa
aaaaa
Film thickness
aaaaa
aaaaa
Login to view more
Abstract
The invention discloses a GC-NCI-MS (gas chromatography-negative chemical ionization-mass spectrometry) determination method of residual amount of tetrachlorantraniliprole. The method is mainly used for determining the residual amount of tetrachlorantraniliprole in cereal grains, animal-derived foods and other complex matrix food agricultural products. The method comprises the following steps: homogenizing and extracting residual tetrachlorantraniliprole in a sample with acetonitrile or an acetonitrile solution containing 1% of acetic acid, performing purification and concentration by a C18/PSA solid-phase extraction column, performing GC-NCI-MS detection, adopting a blank matrix solution without a pesticide to be detected to establish a standard curve for correction, and quantifying by an external standard method. According to the method, the average recovery rate is 83.3%-90.3%, the average relative standard deviation (RSD) is 3.5%-6.9%, and the detection limit is lower than 1.43mu g/kg; and the method has the advantages of simplicity in operation, high speed, good impurity removal effect, high sensitivity, strong characteristic feature, good repeatability and accurate quantitative and qualitative properties. According to the method, the technical requirement of 'being standard without exceptions' of the residual limit of 0.01mg/kg can be met, and the powerful technical support is provided for ensuring food safety of people in China and health development of export trades.
Description
technical field [0001] The present invention relates to a kind of GC-NCI-MS determination method of tetrachlorantraniliprole residual amount, more specifically adopt gas chromatography-negative chemical ion source-mass spectrometry (GC-NCI-MS) to qualitatively and quantitatively measure grain, pork, The invention discloses a method for residual tetraclotraniliprole content in animal and plant-derived foods with complex matrices such as beef, mutton, chicken and other animal muscles and products, and belongs to the technical field of determination of pesticide residues. Background technique [0002] Bisamide insecticides are popular insecticide products in the world in recent years. They can be widely used in the pest control of rice, vegetables, cotton and other crops. They have the advantages of low toxicity, environmental safety and high activity, including chlorantraniliprole , cyantraniliprole, flubendiamide and other varieties, tetrachlorantraniliprole (SYP-9080) is a ne...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more