Method for measuring residual amounts of hormones in cosmetics

A technology for cosmetics and residues, used in measuring devices, instruments, scientific instruments, etc.

Inactive Publication Date: 2014-07-02
XINXIANG MEDICAL UNIV
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  • Abstract
  • Description
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Problems solved by technology

The study found that there is no relevant report on the HPLC-MS

Method used

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  • Method for measuring residual amounts of hormones in cosmetics
  • Method for measuring residual amounts of hormones in cosmetics
  • Method for measuring residual amounts of hormones in cosmetics

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Experimental program
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Embodiment

[0019] (1) Sample processing

[0020] The sample is extracted with cyclohexane, the extraction rate is low; and there is the problem of impurity interference. At present, domestic reports have focused on the sample pretreatment method and the selection of chromatographic conditions, but the cumbersome pretreatment process leads to low recovery. In this experiment, methanol was selected as the extraction solution, and the sample was directly injected after ultrasonic filtration to simplify the pretreatment steps; with water-acetonitrile as the mobile phase, the three hormones could be completely separated. Moreover, under this chromatographic condition, the peaks in the three hormones and the sample can be completely separated.

[0021] (2) Selection of detection wavelength

[0022] The methanol solutions (20μg / mL) of estriol, estradiol and estrone were scanned at full wavelength, and the results showed that the three estrogens had the maximum absorption at 207-280nm, and the...

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Abstract

The invention establishes an analytical method for measuring the residual amounts of estradiol, estriol and oestrone in cosmetics by highly efficient liquid chromatography-tandem mass spectrometry. The method comprises the following steps: with methanol as an extraction solvent, ultrasonically extracting different kinds of cosmetic samples for 30min; centrifuging the sample extracting solution with a speed of 12000r/min for 20min; with acetonitrile and water (in a volume ratio of 40:60) as a mobile phase, performing constant gradient elution, wherein the flow speed is 0.2ml/min and the column temperature is 25 DEG; and performing separation by an EclipseXDB-C18 (with the area of 2.1mm*150mm and the grain size of 5 microns) chromatographic column. The result indicates that the correlation coefficient R of an equation of linear regression of the three hormones is in the range of 0.9983-0.9998, the recycling rate is in the range of 95.21%-106.19% and the accuracy degree is in the range of 1.70%-4.82%. The method for measuring the residual amounts of hormones in cosmetics is simple, agile, rapid, reliable, good in reproducibility and applicable to analysis and detection on the content of hormones in cosmetics.

Description

technical field [0001] The invention relates to a method for processing cosmetics and simultaneously measuring the residual amounts of three estrogens in the cosmetics. Background technique [0002] Estrogen mainly has the function of controlling accessory sexual organs and secondary sexual characteristics, and has different degrees of influence on the metabolism of sugar, fat, protein, salt and other substances in the body. Acting directly on the skin, it can promote hair growth, prevent skin aging, remove wrinkles, and increase skin elasticity. However, long-term use of cosmetics added with estrogen will have harmful effects on the human body and may cause cancer. Long-term use of estrogen-containing cosmetics can lead to diseases such as cell cancer, endometrial cancer, breast cancer, and precocious puberty in children. In 1987, my country formulated the "Hygienic Standards for Cosmetics" (GB7916-1987), the earliest version of the current "Hygienic Standards for Cosmeti...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 闫清华杨理张文博解博红杨丽娟
Owner XINXIANG MEDICAL UNIV
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