36results about How to "In line with biological characteristics" patented technology

The invention relates to a method for preparing a stem cell active factor, and on one hand, in particular relates to a method for preparing the stem cell active factor. The method comprises the following steps of: (1) acquiring umbilical cord placenta derived mesenchymal stem cells; (2) performing cell amplification; (3) performing cell identification detection; (4) preparing the stem cell active factor; and (5) performing optional freeze-drying. Furthermore, the invention relates to a stem cell active factor freeze-dried powder agent prepared by using the method demonstratively. The method provided by the invention has the beneficial effects as shown in the specification of the invention.

The invention relates to a stem cell active factor and lyophilized powder thereof, in particular to a stem cell active factor lyophilized powder on the one hand. The stem cell active factor lyophilized powder comprises a stem cell active factor and a lyophilization protecting agent, and the lyophilization protecting agent is selected from trehalose, mannitol, chitosan, dextran, glycine, arginine and glycine. The invention further relates to a preparation method for preparing the stem cell active factor lyophilized powder. The method has the beneficial effects as described in the specification of the invention.

The invention relates to the bee keeping technology, provides a breeding method for a good bee species for facility crop pollination, and aims at solving the problems that an existing good bee species for facility crop pollination is fewer, poor in quality and poor in pollination effect. The method comprises the steps that local Apis mellifera is bred, a Jolnalof bee species is introduced and bred, a single cross or backcross mode is adopted, and a good variety (strain) which meets the biological property of bees and can be used for effective pollination of facility crops is bred through many times of artificial insemination hybridization. The good properties of being high in collection, high in spawning rate, high in stress resistance, high in disease resistance, docile in temper, low in fodder consumption and the like are achieved, the movement enthusiasm of the pollination bees in a facility crop environment is remarkably improved, crop pollination is effectively achieved, and the pollination effect of the crops is improved.

The invention discloses a semicircular all-metal Chinese bee activity comb frame which is capable of enabling Chinese bees to form spherical bee clusters and suitable for the Chinese bees to store honey and produce honey in supers. The semicircular all-metal Chinese bee activity comb frame is made according to the following steps of: combining a concave metal upper beam and a semicircular metal lower frame via spot welding or bolts, and threading longitudinal and transverse thin iron wires into threading small holes. The comb frame has a semicircular lower part, is capable of enabling the Chinese bees to form spherical bee clusters and accords with the biological characteristics of Chinese bees; only the 1.2-1.5mm thick metal upper beam is suitable for the Chinese bees to store honey and produce honey in supers, so that the honey quality and output of the Chinese bees are improved and the structure is simple.

The invention brings forward a constraining knowledge and elite individual strategy genetic algorithm fusion-based crop growth period model variety parameter optimization method and belongs to the field of crop model variety parameter optimization methods. The method comprises the following steps: data preparation is made for crop growth period model variety parameter optimization; an initial range of crop growth period model variety parameters is graded; a phonological period which has key constraints for the model variety parameters are determined, a measured data range of the phonological period having key constraints is estimated, an improved genetic algorithm is set to control initial parameter value, an initial species population is generated, a fitness function is built, gene direction factors of individuals are built, a selection operator is built via a roulette method, a direction guide crossover operator is built, a direction and elite individual guide mutation operator is built, and elite individual-based local search is conducted. The constraining knowledge and elite individual strategy genetic algorithm fusion-based crop growth period model variety parameter optimization method can help address problems of low efficiency, inconsistency between optimization results and variety biology, and the like; parameter optimization accuracy and parameter optimization efficiency are improved, and the method has scientific significance and practical value.

The invention relates to a freeze-drying method for preserving cytokine activity. The method comprises the steps of selecting a stem cell active factor and a cryoprotectant of which the purity is 99%or more, wherein the cryoprotectant is prepared from, 1-10wt.% of mannitol, 1-3wt.% of trehalose, 1-5wt.% of dextran, 1-3wt.% of human albumin, 1-4wt.% of chitosan, 0.1-0.3wt.% of glycine, 3-4wt.% ofglycine and 0.1-0.3wt.% of an ascorbic acid; preparing cytokine freeze-dried powder by using the stem cell active factor and the cryoprotectant. Compared with the prior art, a cell source of the method has homogeneity and conforms to the biological characteristics of stem cells, thereby ensuring the secretion amount of a stem cell-derived active factor and stability of performance; a medium does not contain sensitizing components, and is suitable for various people; a freeze-dried powder preparation provides convenience for long-distance transportation and long-term preservation.

The invention relates to a facility crop honeybee pollination box which comprises a box body and a box cover, wherein a feeding machine is arranged inside a front panel of the box body; a front panel of the feeding machine is closely combined with the front panel of the box body; two clamps for fixing the direction of the feeding machine are arranged inside the box body; a breathing screen window with a sliding panel is arranged at the bottom of a beehive; the sliding panel is arranged at the bottom of the breathing screen window and is closely combined with a base plate of the box body; an opening is formed in a front panel of the beehive and is used as a hive entrance; a rotating shaft is arranged at the middle part of the hive entrance; two ends of the rotating shaft are cut obliquely. The facility crop honeybee pollination box has the advantages that the beehive is portable, conforms to the biological characteristics of honeybees and is suitable for commercial honeybee pollination operation; by change of a beehive cover, material use is reduced, and the volume of the beehive is decreased; the transparent screen window is ventilating, so that the overheating death rate of the honeybees in a transportation process is reduced; the height of the hive entrance is increased, materials are saved, and transportation is facilitated; the beehive is lower than the feeding machine, so that the facility crop honeybee pollination box is convenient to use, and unprofessional people can supplement feeds easily; the lives of pollination bees are effectively prolonged.

The invention relates to a natural collecting method for Chinese bees escaping from nests. The method includes the steps of firstly, using one queen bee one-way passage sleeve; secondly, allowing one end of the sleeve to be connected to a bee box; thirdly, allowing the other end of the sleeve to be connected to a queen bee and bee colony escaping collecting cage or box; fourthly, shaking off the escaped bee colony in the collecting cage or box into a new bee box for reproduction after the bee colony escapes from the original bee box; fifthly, checking and processing the queen bee and bee colony in the original bee box. The natural collecting method has the advantages that the method is simple to operate, easy to learn and understand, trouble saving and also suitable for collecting western bee varieties, workload of beekeeping is relieved greatly, huge loss caused by Chinese bee escaping is avoided, and the method conforms to the living habits of the Chinese bees and the biological features that the reproductive capacity of the bee colony escaping the nests is multiplied and the disease resistance of the bee colony is increased evidently.

The invention provides a split-combined type honeybee nest box with an arc-shaped bottom, which can quickly check and process abnormal conditions at the lower part of each nest comb in the nest box and naturally discharge wastes in the box, and a use method. The split-combined type honeybee nest box is characterized in that a long-strip-shaped residue discharge hole is formed on a longitudinal central line of a bottom plate of a bottom box of the nest box, wherein, the bottom box is one half of the nest box in height; two arc-shaped plates are mounted in the box; an upper frame of the nest box, which is one half of the nest box in height, is overlapped on the upper surface of the box, and a nest door hole is formed in front of the upper frame; overlapped parts of the upper frame of the nest box and the nest box are connected by using two hinges in the rear, and fixed by using two fasteners in the front; and a semi-circular honeybee movement nest frame with a steel and wood structure, which is invented by the same inventor, is fixed in the nest box. When the split-combined type honeybee nest box is used, the upper frame of the nest box is overturned backwards, and all semi-circular nest combs in the nest box can be overturned at the same time, so that the abnormal conditions at the lower part of each nest comb in the nest box can be quickly checked and processed. The split-combined type honeybee nest box with the arc-shaped bottom accords with biological characteristics of the honeybees, cannot influence normal production and usual migratory raising of the honeybees, is convenient to use and has a simple structure; and numerous honeybees can be raised by one person.

The invention discloses a construction method of a moso bamboo breast-diameter age joint distribution dynamic model. The construction method comprises the following steps: step 1, collecting moso bamboo continuous checking sample plot data in a research area; 2, summarizing the continuous checking data of the moso bamboo sample plot at each stage in the research area into moso bamboo breast-diameter age two-dimensional statistical data; 3, calculating edge distribution values of the diameter at breast height and the age of the moso bamboo; 4, fitting the two-dimensional statistical data of thebreast-diameter age of the moso bamboos in each stage in the step 2 to obtain a parameter vector of each binary Copula density function of each stage of data; 5, obtaining the optimal binary Copula density function of the DBH age of the moso bamboo continuous checking data in each stage; step 6, establishing a linear regression relationship between the binary Copula density function parameter andthe period number; and step 7, obtaining a moso bamboo breast-diameter age joint distribution dynamic model. The invention has the advantages that the regional scale moso bamboo biomass can be accurately predicted, the dynamic change of the moso bamboo breast-diameter age can be reflected, and a theoretical basis is provided for carbon transaction and scientific operation of a moso bamboo forest.

The invention provides a bionic natural bee colony queen breeding device, a manufacturing method thereof and a bee colony queen breeding method. The bionic natural bee colony queen breeding device comprises a first queen breeding plate assembly, a second queen breeding plate assembly and a third queen breeding plate assembly, the surface, deviating from the opening of a first worker honeycomb, of a first spawning plate of the first queen breeding plate assembly is connected with the surfaces of a plurality of first queen breeding strips; the surface, deviating from the opening of a second worker honeycomb, of a second spawning plate of the second queen breeding plate assembly is connected with the surfaces of a plurality of second queen breeding strips; and the surface, deviating from the queen cell opening of a second queen cell base, of a third spawning plate of the third queen breeding plate assembly is connected with the surfaces of a plurality of third queen breeding strips. The bionic natural bee colony queen breeding device is more reasonable and more scientific in structural design, simple to use and easy to master by common beekeepers, conforms to the biological characteristics of bee colonies, and can breed queens more easily.

The invention discloses a zoological-imitated fishway with a natural cobble partition plate. The zoological-imitated fishway comprises fishway partition plates and a fishway aqueduct body, wherein vertical side walls are arranged on the left sides of the fishway partition plates; inclined side walls are arranged on the right sides of the fishway partition plates; the fishway partition plates comprise cobble partition walls, long partition plates, short partition plates and longitudinal pore plates; the cobble partition walls are connected with one side of the inclined side wall; S-shaped fishthrough holes are formed in different areas at different heights of the cobble partition walls; the long partition plates are connected with the other ends of the cobble partition plates; the longitudinal pore plates are connected with one sides of the long partition plates; the long partition plates and the short partition plates are distributed in a staggered manner to form fish through verticalseams. By adopting the zoological-imitated fishway, harmonies of through fishes and fishway water flows are improved, the fish through efficiency is improved, and a nature-imitated effect is achieved; in addition, due to adoption of the 'S'-shaped fish through holes, a flow field structure similar to a through fish target swimming track line is constructed, biological characteristics of target fishes are met, and furthermore, the purpose that the flow speed of a chamber is reduced and upstream sailing of fish targets is met are achieved.

The invention discloses a variable-rigidity soft pneumatic rehabilitation hand. The rehabilitation hand comprise a glove, soft drivers are arranged on five fingers of the glove, each soft driver comprises a soft driver cavity, a cavity is formed in the bottom of each soft driver cavity and connected to an air pump through a hose, the upper surface of each driver cavity is in a comb tooth shape, and the comb tooth density at the finger joints is smaller than that at the finger backs. The rehabilitation hand can help a patient with disabled hands to do hand exercising.

The invention discloses a short-frame multilayer multipurpose Apis cerana hive. A first following hive is placed on a bottom hive, a second following hive is placed on the first following hive, a queen excluder is inserted between the first following hive and the second following hive, a wire gauze auxiliary lid covers the upper portion of the second following hive, air holes are formed in a hivelid, wood partition strips fixedly mounted in the hive lid are abutted onto the wire gauze auxiliary lid, the hive lid covers the upper portions of the second following hive and the wire gauze auxiliary lid, a hive bottom plate is placed on the lower portion of the square bottom hive, and wooden spacers are arranged at lower ends of the hive bottom plate. According to the hive, the heights of hivebodies are consistent, frames can be exchanged among the hive bodies at random, multilayer following hives can be produced, breeding areas and honey production areas of swarm can be separated, swarmbreeding cannot be affected, the purity degree of honey cannot be affected, the yield and the concentration of the honey can be improved, and high-quality honey meeting market requirements can be provided.