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Freeze-drying method for preserving cytokine activity

A cytokine and freeze-drying technology, applied in the field of cells, can solve the problems of restricting the application of stem cell active factors and short storage time of stem cell growth factor activity, and achieve the effect of facilitating long-distance transportation, facilitating long-term storage, and ensuring the secretion amount.

Inactive Publication Date: 2019-04-16
深圳光彩生命工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, under the existing technology, the activity preservation time of stem cell growth factors is very short, which greatly limits the application of stem cell activity factors, so it is necessary to solve these problems

Method used

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  • Freeze-drying method for preserving cytokine activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Such as figure 1 Shown, a kind of freeze-drying method of preserving cytokine activity comprises the following steps:

[0024] Stem cell activity factor and cryoprotectant with a purity greater than or equal to 99% are selected, wherein the cryoprotectant is composed of raw materials with the following concentrations: 10wt.% mannitol, 1wt.% trehalose, 1wt.% dextran, 1wt.% human blood Albumin, 1wt.% chitosan, 0.1wt.% glycine, 3wt.% glycine and 0.1wt.% ascorbic acid;

[0025] Cytokine freeze-dried powder was prepared by using stem cell active factor and cryoprotectant.

[0026] In the above-mentioned embodiment, selecting the stem cell active factor with a purity greater than or equal to 99% includes the following steps:

[0027] Obtain umbilical cord-derived mesenchymal stem cells, and culture and expand the umbilical cord-derived mesenchymal stem cells;

[0028] When a part of the umbilical cord-derived mesenchymal stem cells are cultured to the peak, the supernatant...

Embodiment 2

[0035] Such as figure 1 Shown, a kind of freeze-drying method of preserving cytokine activity comprises the following steps:

[0036] Stem cell activity factor and cryoprotectant with a purity greater than or equal to 99% are selected, wherein the cryoprotectant is composed of raw materials with the following concentrations: 1wt.% mannitol, 3wt.% trehalose, 5wt.% dextran, 3wt.% human blood Albumin, 4wt.% chitosan, 0.3wt.% glycine, 4wt.% glycine and 0.3wt.% ascorbic acid;

[0037] Cytokine freeze-dried powder was prepared by using stem cell active factor and cryoprotectant.

[0038] In the above-mentioned embodiment, selecting the stem cell active factor with a purity greater than or equal to 99% includes the following steps:

[0039] Obtain umbilical cord-derived mesenchymal stem cells, and culture and expand the umbilical cord-derived mesenchymal stem cells;

[0040] When a part of the umbilical cord-derived mesenchymal stem cells are cultured to the peak, the supernatant ...

Embodiment 3

[0047] Such as figure 1 Shown, a kind of freeze-drying method of preserving cytokine activity comprises the following steps:

[0048] Stem cell activity factor and cryoprotectant with a purity greater than or equal to 99% are selected, wherein the cryoprotectant is composed of raw materials with the following concentrations: 6wt.% mannitol, 2wt.% trehalose, 3wt.% dextran, 2wt.% human blood Albumin, 3wt.% chitosan, 0.2wt.% glycine, 4wt.% glycine and 0.2wt.% ascorbic acid;

[0049] Cytokine freeze-dried powder was prepared by using stem cell active factor and cryoprotectant.

[0050] In the above-mentioned embodiment, selecting the stem cell active factor with a purity greater than or equal to 99% includes the following steps:

[0051] Obtain umbilical cord-derived mesenchymal stem cells, and culture and expand the umbilical cord-derived mesenchymal stem cells;

[0052] When a part of the umbilical cord-derived mesenchymal stem cells are cultured to the peak, the supernatant ...

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Abstract

The invention relates to a freeze-drying method for preserving cytokine activity. The method comprises the steps of selecting a stem cell active factor and a cryoprotectant of which the purity is 99%or more, wherein the cryoprotectant is prepared from, 1-10wt.% of mannitol, 1-3wt.% of trehalose, 1-5wt.% of dextran, 1-3wt.% of human albumin, 1-4wt.% of chitosan, 0.1-0.3wt.% of glycine, 3-4wt.% ofglycine and 0.1-0.3wt.% of an ascorbic acid; preparing cytokine freeze-dried powder by using the stem cell active factor and the cryoprotectant. Compared with the prior art, a cell source of the method has homogeneity and conforms to the biological characteristics of stem cells, thereby ensuring the secretion amount of a stem cell-derived active factor and stability of performance; a medium does not contain sensitizing components, and is suitable for various people; a freeze-dried powder preparation provides convenience for long-distance transportation and long-term preservation.

Description

technical field [0001] The invention relates to the technical field of cells, in particular to a freeze-drying method for preserving cytokine activity. Background technique [0002] Stem cells are a type of pluripotent cells with self-replicating ability. Under certain conditions, it can differentiate into a variety of functional cells. Stem cells are divided into embryonic stem cells and adult stem cells according to their developmental stage. According to the developmental potential of stem cells, they are divided into three categories: totipotent stem cells, pluripotent stem cells and unipotent stem cells. Stem cells are the cells of origin. Stem cells have the ability to self-renew and replicate, and can produce highly differentiated functional cells. [0003] At the same time, studies have shown that stem cell growth factors can quickly activate dormant stem cells and promote their growth, and at the same time can regulate the microenvironment in the body to provide...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 陈阳丁小梅
Owner 深圳光彩生命工程技术有限公司
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