Identification method for porphyra haitanensis in different harvesting periods

Abstract

The invention relates to a method for identifying porphyra haitanensis in different harvesting periods. The method comprises the steps of pretreatment, smashing, centrifugation, suction filtration, vacuum freeze drying, nuclear magnetism spectrum collection and data analysis and identification. The method specifically comprises the following steps: performing Fourier transformation on all one-dimensional 1H-NMR spectrum data of a sample to be identified to obtain a spectrogram, then performing signal assignment, phase adjustment, base line correction, calibration and integration, and performing multi-variable data analysis on the normalized NMR data by using SIMCA-P+ software; performing main component analysis on the NMR data, performing orthogonal partial least squares discriminant analysis on the NMR data, and taking the automatically normalized NMR data as an X variable and grouping information as a Y variable. PCA analysis results of the NMR data of extracts of the porphyra haitanensis in different harvesting periods are obviously different, and nori harvested in the previous period and nori harvested in the middle period are mainly distinguished in PC2; however, nori harvested in the later period is mainly different from the nori harvested in the previous period and the nori harvested in the middle period in PC1.

Description

Technical field [0001] The invention belongs to the technical field of the detection of the growth period of algae, and specifically relates to a method for identifying Porphyra haitanensis in different harvest periods. Background technique [0002] Pyropiahaitanensis belongs to red algae and is one of the main seaweed species cultivated in my country. It is an ideal high-protein, low-fat food, rich in a variety of amino acids and inorganic salts, and is loved by consumers. However, the harvesting period of seaweed lasts longer, and the quality of seaweed in different harvesting periods is different, and the market price varies greatly. Generally speaking, the laver harvested for the first time (time around mid-November) is called one water laver, the laver harvested for the second time (time around late December) is called two water laver, and the third time is harvested. (The time is about late January of the following year) Laver is called Sanshui Laver. However, it is diffi...

AI Technical Summary

Problems solved by technology

However, it is difficult for consumers to distinguish laver in different harvest periods in terms of morphology. Therefore, it is necessary to provide an effective method for detecting laver in different harvest periods.

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