Molecular markers for identifying high temperature-resistant porphyra haitanensis strain and construction method for molecular markers

A technology of high temperature resistance and molecular markers, applied in the field of laver, can solve the problems of limited morphological traits, germplasm confusion, unfavorable promotion of improved varieties of algae and protection of intellectual property rights, etc.

Inactive Publication Date: 2012-09-12
JIMEI UNIV
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Problems solved by technology

However, because the selected Porphyra high-temperature-resistant strains have no obvious difference in morphology from other Porphyra strains, the morphological characters that can be used for identification are very limited, which often causes germplasm confusion in production, which is not conducive to the promotion and development of improved Porphyra varieties. Intellectual Property Protection

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  • Molecular markers for identifying high temperature-resistant porphyra haitanensis strain and construction method for molecular markers
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  • Molecular markers for identifying high temperature-resistant porphyra haitanensis strain and construction method for molecular markers

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Embodiment Construction

[0068] The following embodiments will further illustrate the present invention in conjunction with the accompanying drawings.

[0069] The construction method of the molecular markers for identifying the high-temperature-resistant strains of Porphyra alba is as follows:

[0070] 1) Collection of algae laver materials: collect fresh and healthy frond materials of algae laver high-temperature-resistant and non-high-temperature-resistant strains, treat them with sterile seawater, and set aside;

[0071] 2) Total DNA extraction: the collected thallus material was ground into powder in liquid nitrogen, and the total DNA was extracted by CTAB method;

[0072] 3) RAPD scanning analysis: use RAPD primers to carry out marker scanning analysis on the total DNA of each material obtained in step 2). The reaction system for RAPD amplification is: 10×PCR buffer 2.0 μL, 2.5mM dNTP 1.0 μL, 10 μM RAPD primer 1.0 μL, 25mM MgCl 2 1.0μL, 5U / μL Taq DNA polymerase 0.3μL, 5ng / μL template DNA 2.0μL...

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Abstract

The invention discloses molecular markers for identifying a high temperature-resistant porphyra haitanensis strain and a construction method for the molecular markers and relates to porphyra haitanensis. The molecular markers for identifying the high temperature-resistant porphyra haitanensis strain are Z-26-600 and Z-26-360. The construction method comprises the following steps of: collecting porphyra haitanensis materials; extracting total deoxyribose nucleic acid (DNA); performing random amplified polymorphic (RAPD) sweep analysis; performing electrophoresis detection of an RAPD amplification product; performing gel extraction on specific bands and recovering, and sequencing; designing sequence characterized amplified region (SCAR) maker primes; and verifying SCAR markers. A method foridentifying the high temperature-resistant strain by using the SCAR markers comprises the following steps of: collecting the porphyra haitanensis materials; extracting total DNA; performing polymerase chain reaction (PCR) amplification; performing electrophoresis detection of a PCR amplification product; and judging according to a result.

Description

technical field [0001] The present invention relates to a kind of algae laver, in particular to a molecular marker and a construction method thereof for identifying a high temperature-resistant strain of algae laver. Background technique [0002] Porphyra haitanensis is a unique species of warm temperate zone in my country. It is an important economic red algae artificially cultivated in coastal areas of Fujian, Zhejiang and Guangdong. Its output accounts for about 80% of the total national production of laver. [0003] The natural growth period of altar laver is from September to March of the next year, and the suitable temperature for growth is 15-26°C. In recent years, global climate warming, the continuous high temperature after the Bailu solar term and the continuous high temperature recovery from late September to October in the coastal areas of Fujian and Zhejiang have seriously affected the harvesting and attachment of seedlings of Porphyra porphyra, and the water te...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10
Inventor 谢潮添陈昌生纪德华徐燕王婷
Owner JIMEI UNIV
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