Establishment method of porphyra haitanensis core germplasm bank

A technology of core germplasm and construction method, which is applied in the field of construction of the core germplasm bank of Porphyra chinensis, can solve the problems of disappearance of wild elite germplasm and destruction of wild Porphyra chinensis resources, etc.

Inactive Publication Date: 2016-12-14
JIMEI UNIV
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Problems solved by technology

As the basis of Porphyra genetics and breeding, wild Porphyra is rich in genetic diversity. However, in recent years, with the development of sea areas and artificial indiscriminate harvesting, the resources of wild Porphyra have been seriously damaged, and the wild excellent germplasm is gradually disappearing.

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  • Establishment method of porphyra haitanensis core germplasm bank
  • Establishment method of porphyra haitanensis core germplasm bank
  • Establishment method of porphyra haitanensis core germplasm bank

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specific Embodiment

[0034] Construction of the core germplasm bank of wild Porphyra alba in coastal Fujian

[0035] 1. Collection and purification of Porphyra germplasm:

[0036] (1) Collect 77 wild Porphyra germplasm samples from all over the coast of Fujian.

[0037] (2) Take the collected algae germplasm samples, take a single plant thallus, clean the surface with a brush, then dry it in the shade and store it in a freezer (-18°C); take out the frozen algae, and wash it again with sterilized seawater Afterwards, they were revived in still water and weak light for 24 hours (cultivation conditions: temperature 20±1°C, light 1000Lx, 12L:12D).

[0038] (3) Use sterilized scissors to cut 0.5-1cm 2 The vegetative tissue blocks of the algae were soaked in 2mol / L glucose solution for 10 minutes, then cut the algae blocks into small pieces with scissors, and placed in the conch enzyme solution for enzymatic hydrolysis (enzyme liquid formula: 0.12g conch enzyme + 1.27 g mannitol + 2.2mg anhydrous CaC...

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Abstract

The invention provides an establishment method of a porphyra haitanensis core germplasm bank and relates to porphyra haitanensis. The method includes the following steps that porphyra haitanensis germplasm is collected and purified; 2, strain genome DNA of all the germplasm of porphyra haitanensis is extracted; 3, porphyra haitanensis germplasm strain genetic diversity analysis based on an SSR molecular marker is carried out; 4, the genetic similarity coefficient between all germplasm strains is calculated; 5, gradual clustering sampling is carried out to establish the core germplasm bank. The obtained core germplasm bank can preserve the genetic diversity of original germplasma to the maximum degree with a smallest sample number, therefore, parent assembly links carried out during following porphyra haitanensis crossbreeding are simplified, and the breeding efficiency and the breeding effect are improved.

Description

technical field [0001] The invention relates to algae laver, in particular to a method for constructing an almanac laver core germplasm bank. Background technique [0002] Pyropia haitanensis is native to the rocks in the intertidal zone along the southern coast of my country, and is a unique warm temperate species in China. Since the success of artificial cultivation in the 1960s, the cultivation area of ​​algae laver has continued to expand. At present, it has become one of the main targets of coastal mariculture in southern Zhejiang, Fujian and northern Guangdong, creating considerable economic benefits. [0003] Marine biological germplasm resources are the basis of "blue agriculture". As the basis of Porphyra genetics and breeding, wild Porphyra is rich in genetic diversity. However, in recent years, with the development of sea areas and artificial indiscriminate harvesting, the resources of wild Porphyra have been seriously damaged, and the wild excellent germplasm is...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C40B50/06
CPCC12Q1/6895C12Q1/6811C40B50/06C12Q2537/165C12Q2531/113
Inventor 谢潮添陈昌生徐燕纪德华
Owner JIMEI UNIV
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