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Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.

A breeding method and technology for new varieties, applied in the field of new variety cultivation that induces mutations in rhizome slices of Aralia japonica, can solve the problems of low artificial cultivation yield, late bud germination, high thorn hardness, etc., and achieve the purity of genetic information of a single plant High, high mutation rate, high dominant mutation effect

Active Publication Date: 2020-06-23
TONGHUA NORMAL UNIV +1
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  • Claims
  • Application Information

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Problems solved by technology

[0005] In order to solve the above-mentioned problems such as the depletion of wild resources of Aralia aralia, low artificial cultivation yield, late bud germination, small buds, easy aging, high thorn hardness, serious plant degradation, and insect damage, the invention provides an induced Aralia aralia Cultivation Method of New Variety with Mutation of Rhizome Slice Tissue

Method used

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  • Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.
  • Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.
  • Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.

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Embodiment Construction

[0029] The technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments.

[0030] like figure 1 and2 As shown, a new variety cultivation method for inducing tissue mutation of rhizomes of Aralis japonica Liaodong comprises the following steps:

[0031] 1 Materials and methods

[0032] 1.1 Material selection and pretreatment

[0033] In the middle and late October, choose the rhizome with a diameter of about 0.3cm of wild Aralis japonica, wash a small amount of soil on the surface of the rhizome with sterile water and a soft brush, cut it into a 1.0cm stem section and use 75% ethanol (volume percent concentration). ) rinsed for 15s, moved to saturated sodium hypochlorite solution and soaked for 10min, rinsed 15 times with sterile w...

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Abstract

The invention belongs to the technical field of plant propagation, in particular to a method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.. According to the method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem. disclosed by the invention, by using the rhizomes of the Aralia elata(Miq)Seem.as materials, the rhizomes are soaked with ethyl methanesulfonate with appropriate concentration for a certain time, then a plant tissue culturing technology is combined, and methods such as inductionof rhizome slice tissue buds (cormus) as well as rooting and rejuvenation growth of the buds (cormus) are adopted, so that the novel artificial cultured varieties of the Aralia elata(Miq)Seem. havingthe characteristics of being high in independence of each single plant, high in purity of genetic information of the single plant and clear in source of single plant can be obtained. The varieties are stable in genetic property, and can be applied to variety-line cultivation and building of mutant libraries of Aralia elata(Miq)Seem.

Description

technical field [0001] The invention belongs to the technical field of new plant varieties, and in particular relates to a new variety cultivation method for inducing tissue mutation of rhizomes of Aralis japonica in Liaodong. Background technique [0002] Liaodong aralia [ralia elata (Miq) Seem.] is a perennial deciduous shrub or small tree of the Araliaceae family, alias: thorn dragon bud, thorn old bud, thorn tender bud, dragon bud aralia, magpie do not step and so on. 1.5-6 meters high, bark gray; branchlets gray-brown, with many sparse thorns; thorns 1-3 mm long, base swollen; shoots often have slender straight thorns up to 1.5 cm. Liaodong Aralia grows in forests at an altitude of about 1000 meters. It likes cool and humid climates. It is a negative tree species. It mostly grows on shady slopes, mainly in the middle and upper parts of the slopes, generally under forests, forest edges and forests. On both sides of the open space and the inter-forest logging operation, ...

Claims

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Application Information

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IPC IPC(8): A01H4/00A01H1/06A01H1/04
CPCA01H4/008A01H1/06A01H1/04Y02P60/14
Inventor 顾地周王安妮王巨海朱俊义姜冬雪
Owner TONGHUA NORMAL UNIV
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