Method for culturing new varieties for inducing mutation of rhizome slice tissues of Aralia elata(Miq)Seem.
A breeding method and technology for new varieties, applied in the field of new variety cultivation that induces mutations in rhizome slices of Aralia japonica, can solve the problems of low artificial cultivation yield, late bud germination, high thorn hardness, etc., and achieve the purity of genetic information of a single plant High, high mutation rate, high dominant mutation effect
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[0029] The technical solutions in the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, but not all of the embodiments.
[0030] like figure 1 and2 As shown, a new variety cultivation method for inducing tissue mutation of rhizomes of Aralis japonica Liaodong comprises the following steps:
[0031] 1 Materials and methods
[0032] 1.1 Material selection and pretreatment
[0033] In the middle and late October, choose the rhizome with a diameter of about 0.3cm of wild Aralis japonica, wash a small amount of soil on the surface of the rhizome with sterile water and a soft brush, cut it into a 1.0cm stem section and use 75% ethanol (volume percent concentration). ) rinsed for 15s, moved to saturated sodium hypochlorite solution and soaked for 10min, rinsed 15 times with sterile w...
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