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A kind of method of ethyl methanesulfonate mutagenesis treatment Andrographis paniculata

A technology of ethyl methanesulfonate and Andrographis paniculata, applied in the fields of seed and rhizome treatment, botanical equipment and methods, plant genetic improvement, etc., can solve the problem of uneven wax thickness, uneven water absorption capacity of seeds, and low germination potential of seeds and other issues to achieve the effect of improving the efficiency of the industry

Active Publication Date: 2021-07-23
CROP RES INST GUANGDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The thickness of the wax on the surface of Andrographis paniculata seeds varies, and the water absorption capacity of the seeds is uneven, resulting in low germination potential of the seeds in production

Method used

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  • A kind of method of ethyl methanesulfonate mutagenesis treatment Andrographis paniculata
  • A kind of method of ethyl methanesulfonate mutagenesis treatment Andrographis paniculata
  • A kind of method of ethyl methanesulfonate mutagenesis treatment Andrographis paniculata

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Effect test

Embodiment 1

[0027] 1 Materials and methods

[0028] 1.1 Test material

[0029] Andrographis paniculata seeds were donated by Wen Chunxiu, a researcher of Hebei Academy of Agriculture and Forestry Sciences, and used for production in Zhangzhou, Fujian.

[0030] 1.2 Test method

[0031] 1.2.1 Andrographis paniculata seed treatment

[0032] Soak the Andrographis paniculata seeds in an aqueous solution with 1% detergent, put them into an ultrasonic cleaner for 60 minutes, wash them with water several times until there is no foam, then soak them in water for 12 hours, take out and dry the surface of the Andrographis paniculata seeds with gauze. moisture. The treated seeds were put into EMS solutions with mass fractions of 0.5% and 0.8%, respectively, and cultured with constant temperature shaking for 4h, 8h and 12h. In October 2019, it was planted in a culture soil with a ratio of peat soil and perlite of 3:1, and it was planted and cultivated in an incubator with a temperature of 26° and ...

Embodiment 2

[0063] A method for mutagenic treatment of Andrographis paniculata seeds with ethyl methanesulfonate, comprising the steps of:

[0064] (1) Putting Andrographis paniculata seed into the aqueous solution that adds mass fraction and be the detergent of X and soak Y hour completely in the aqueous solution of liquid detergent of X earlier, carry out ultrasonic treatment then, ultrasonic treatment condition is: treatment frequency is 24kHz, and treatment power is 160W, and treatment time is For 30 minutes, the treatment temperature was room temperature; the ultrasonically treated Andrographis paniculata was washed several times with clear water until there was no foam, and then the Andrographis paniculata seeds were soaked in water for 8 hours, and then the moisture on the surface of the Andrographis paniculata seeds was taken out and dried.

[0065] (2) put the dried Andrographis paniculata seeds into 0.5% ethyl methanesulfonate solution for immersion treatment for 4 hours, then ta...

Embodiment 3

[0072] A method for mutagenic treatment of Andrographis paniculata seeds with ethyl methanesulfonate, comprising the steps of:

[0073] (1) put Andrographis paniculata seed into the aqueous solution that is added with the mass fraction of 0.5% detergent and soak completely for 2 hours, then carry out ultrasonic treatment, the condition of ultrasonic treatment is: treatment frequency is 24kHz, treatment power is 160W, treatment time for 30 minutes, and the treatment temperature is room temperature; wash the Andrographis paniculata after ultrasonic treatment several times with clear water until there is no foam, then soak the Andrographis paniculata seeds in water for 8 hours, then take out and dry the moisture on the surface of the Andrographis paniculata seeds.

[0074] (2) put the dried Andrographis paniculata seeds into 0.5% ethyl methanesulfonate solution for immersion treatment for 4 hours, then take them out and rinse them with clear water, and plant them in peat soil and ...

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Abstract

A method for mutagenic treatment of andrographis paniculata seeds with ethyl methanesulfonate, comprising the following steps: firstly put andrographis paniculata seeds into an aqueous solution added with detergent and completely soak them, then perform ultrasonic treatment, and wash the andrographis paniculata seeds after ultrasonic treatment with clear water Wash several times until there is no foam, then soak the Andrographis paniculata seeds in water, take out and dry the moisture on the surface of the Andrographis paniculata seeds; put the dried Andrographis paniculata seeds into ethyl methanesulfonate solution for soaking, then take them out and rinse with water, sow, Germinate to grow into M0 generation andrographis paniculata plants. The present invention uses ethyl methylsulfonate mutagen for the mutagenesis of Andrographis paniculata for the first time, and according to the characteristics of the seeds and the action principle of EMS, the pretreatment of the seeds is carried out first, and then the weak base treatment, ultrasonic treatment and methylsulfonate Combined with ethyl acetate mutagenesis to mutate Andrographis paniculata seeds, a mutant library covering the whole genome can be constructed, which provides a material basis for basic research such as functional genomics and metabolomics.

Description

technical field [0001] The invention relates to the field of medicinal plant breeding, in particular to a method for mutagenic treatment of andrographis paniculata with ethyl methanesulfonate. Background technique [0002] Andrographis paniculata (Burm.f.) Nees is an annual herb of the Acanthaceae family, one of the traditional bulk Chinese medicinal materials in my country, rich in diterpene lactones such as andrographolide, deoxyandrographolide and neoandrographolide , has anti-inflammatory, anti-bacterial, anti-virus, infection and other effects. At present, the quality of Andrographis paniculata products is not uniform, the species degenerates, and the content of effective active ingredients decreases. Breeding new varieties with higher active ingredients is an effective way to improve the quality of Andrographis paniculata. Among them, chemical mutation breeding is usually based on point mutation, which is simple to operate, the mutant has stable heredity, and the muta...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01C1/00
CPCA01C1/00A01H1/06
Inventor 王继华周芳梅瑜顾艳杨乃博徐世强蔡时可
Owner CROP RES INST GUANGDONG ACAD OF AGRI SCI
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