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Breeding method for tetraploid maize

A technology of tetraploid corn and corn, which is applied in the fields of botany equipment and methods, applications, plant gene improvement, etc., can solve the problems of high preparation failure rate, high production cost, and inability to achieve precise mutagenesis, etc., and achieve broad market prospects , high mutagenesis efficiency, and easy to promote on a large scale

Inactive Publication Date: 2016-03-23
HUIAN KELIAN AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method cannot achieve precise mutagenesis, the failure rate of preparation is high, and the production cost is high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of breeding method of tetraploid maize

[0025] The specific steps include seed selection, seed germination cultivation, mutagenesis and seedling cultivation. The mutagenesis is carried out by means of chemical mutagenesis; the mutagens used in the chemical mutagenesis include: 0.1 part of colchicine, 0.1 part of auxin, 0.1 part of naphthalene ethane, 0.1 part of ethyl methanesulfonate, ethylene 0.1 part of imine, 0.1 part of nitrosoethylurethane, 0.1 part of nitrosomethylurethane, 0.1 part of diethyl sulfate, 1 part of diazoserine, 1 part of mitotic toxin C, 1000 parts of purified water. The configuration process of the mutagen is: dissolve the colchicine according to the dosage part in the purified water according to the dosage part, and then add in turn auxin, naphthalene ethane, ethyl methanesulfonate, ethyleneimine, nitroso Ethyl urethane, nitrosomethyl urethane, diethyl sulfate, diazoserine, mitotoxin C, and finally adjust the pH of the mutagen solution wi...

Embodiment 2

[0027] A kind of breeding method of tetraploid maize

[0028] The specific steps include seed selection, seed germination cultivation, mutagenesis and seedling cultivation. The mutagenesis is carried out by means of chemical mutagenesis; the mutagens used in the chemical mutagenesis include: 0.3 parts of colchicine, 0.3 parts of auxin, 0.3 parts of naphthalene ethane, 0.3 parts of ethyl methanesulfonate, ethylene 0.3 part of imine, 0.3 part of nitrosoethylurethane, 0.3 part of nitrosomethylurethane, 0.3 part of diethyl sulfate, 3 parts of diazoserine, 5 parts of mitotic toxin C, 5000 parts of purified water. The configuration process of the mutagen is: dissolve the colchicine according to the dosage part in the purified water according to the dosage part, and then add in turn auxin, naphthalene ethane, ethyl methanesulfonate, ethyleneimine, nitroso Ethyl urethane, nitroso methyl urethane, diethyl sulfate, diazoserine, mitotoxin C, and finally adjust the pH of the mutagen solu...

Embodiment 3

[0030] A kind of breeding method of tetraploid maize

[0031]The specific steps include seed selection, seed germination cultivation, mutagenesis and seedling cultivation. The mutagenesis is carried out by means of chemical mutagenesis; the mutagens used in the chemical mutagenesis include: 0.2 parts of colchicine, 0.2 parts of auxin, 0.2 parts of naphthalene ethane, 0.2 parts of ethyl methanesulfonate, ethylene 0.2 part of imine, 0.2 part of nitrosoethylurethane, 0.2 part of nitrosomethylurethane, 0.2 part of diethyl sulfate, 2 parts of diazoserine, 3 parts of mitotic toxin C, 3000 parts of purified water. The configuration process of the mutagen is: dissolve the colchicine according to the dosage part in the purified water according to the dosage part, and then add in turn auxin, naphthalene ethane, ethyl methanesulfonate, ethyleneimine, nitroso Ethyl urethane, nitrosomethyl urethane, diethyl sulfate, diazoserine, mitotoxin C, and finally adjust the pH of the mutagen soluti...

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Abstract

The invention discloses a breeding method for tetraploid maize. The breeding method comprises the steps of seed selection, seed germination culture, mutagenesis and seedling culture, wherein the mutagenesis step adopts chemical mutagenesis; the mutagenesis method of a maize tetraploid is high in mutagenesis success rate, and free of seedling rotting. A mutagen adopted in chemical mutagenesis comprises a mixture of colchicine, heteroauxin, periethylenenaphthalene, ethyl methanesulfonate, ethylenimine, nitroso-ethyl-urethane, nitroso-methyl-urethane, diethyl sulphate, azaserine, and mitomycin C. The mutagenesis method of the maize tetraploid is high in mutagenesis success rate, and free of seedling rotting, and has a wide market prospect; the chemical mutagen adopted in the mutagenesis method is obtained via precise proportioning, and can realize accurate induction of tetraploid maize; the treated maize is high in yield, short in growth cycle, and has competitive advantage; the breeding method is simple to operate, and easy for wide-range popularization.

Description

technical field [0001] The invention belongs to the field of plant breeding, in particular to a tetraploid corn breeding method. Background technique [0002] Artificial mutation breeding has become one of the important means of modern breeding science and has played a huge role in agricultural production. Therefore, in recent decades, people have conducted in-depth research on polyploidy, which not only provides a lot of evidence for evolution, but also has extensive application value in production practice. With the deepening of the research and utilization of maize heterosis, the requirements for breeding materials are getting higher and higher. Maize tetraploids have large particles and high yields, and have a good prospect of increasing production. Applying mutation breeding to actual maize breeding work It is one of the key points of current breeding work. However, most of the mutagens currently used have simple components, and the accuracy of mutagenesis to plants i...

Claims

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Application Information

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IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 肖礼池
Owner HUIAN KELIAN AGRI TECH CO LTD
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