Method for treating amaranth seeds by adopting ethyl methanesulfonate

A technology of ethyl methanesulfonate and amaranth, applied in the field of crop breeding, can solve the problems of different treatment time requirements, high toxicity of EMS mutagenesis treatment, large difference in suitable treatment concentration, etc., and achieves simple and effective breeding operation process, The effect of simplifying the mutagenesis breeding process and reducing the harm to the human body and the environment

Pending Publication Date: 2018-11-23
NANJING AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

However, there are many problems in the EMS mutagenesis treatment, such as high toxicity, large differen...

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  • Method for treating amaranth seeds by adopting ethyl methanesulfonate
  • Method for treating amaranth seeds by adopting ethyl methanesulfonate

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Embodiment 1

[0027] Prepare a phosphate buffer solution with a pH of 7.0 and a concentration of 0.2 mol / l, filter and sterilize, transfer the mother liquor of ethyl methanesulfonate into the buffer solution with a 200 μl pipette gun, and prepare the volume fractions of 0, 0.2%, and 0.4%, 0.6%, and 0.8% ethyl methanesulfonate solutions; select four parts of amaranth seeds with uniform size and full grains as the initial receptor material for mutagenesis treatment, and put them into different volume fractions of methanesulfonic acid Treat in ethyl ester solution for 8 hours on a shaker at a temperature of 25°C and an oscillation frequency of 120r / min; soak the treated seeds in 0.2M sodium thiosulfate for 20 minutes to detoxify to terminate the reaction, and then rinse with running water for 3 times. Dry the seeds naturally, then transfer the amaranth seeds to a petri dish covered with 3 layers of wet filter paper, and continue to cultivate under the conditions of 20-25°C, light 12h / d, and lig...

Embodiment 2

[0033] Prepare a phosphate buffer solution with a pH of 7.0 and a concentration of 0.2 mol / l, filter and sterilize, transfer the mother liquor of ethyl methanesulfonate to the buffer solution with a 200 μl pipette gun, and prepare four parts of formazan with a volume fraction of 0.6%. Ethyl methanesulfonate solution; select four parts of amaranth seeds with uniform size and full grains as the initial receptor material for mutagenesis treatment, and put them into ethyl methanesulfonate solution respectively, at a temperature of 25 °C and an oscillation frequency of Treat on a shaker at 120r / min for 4h, 8h, and 12h; soak the treated seeds in 0.2M sodium thiosulfate for 20min to detoxify to terminate the reaction, and then rinse with running water for 3 times. Dry the seeds naturally, then transfer the amaranth seeds to a petri dish covered with 3 layers of wet filter paper, and continue to cultivate them for several days under the conditions of 20-25°C, light 12h / d, and light int...

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Abstract

The invention belongs to the field of crop seed breeding and in particular relates to a method for treating amaranth seeds by adopting ethyl methanesulfonate. According to the method provided by the invention, the amaranth seeds are selected as a starting receptor material for mutagenic treatment, and the amaranth seeds are subjected to the mutagenic treatment by utilizing an ethyl methanesulfonate solution with the concentration of 0 to 0.8 v/v percent; the amaranth seeds subjected to the mutagenic treatment are transferred into a culture dish paved with three layers of wet filter paper, andare continually cultured for several days under the conditions that the temperature is 20 to 25 DEG C, the illumination time is 12h/d and the intensity is 1500 to 2000lx; then the sprouted amaranth seeds are transferred into a hole disc with 72 holes and are continually cultured until the seeds grow to an M0 generation, and mutants are screened from the seeds. By adopting the method provided by the invention, the limitation of an existing seed breeding technology is overcome, and the mutagenic effect of the methanesulfonate on the amaranth seeds is improved; the method plays an active role onimprovement of the seed breeding efficiency of amaranths and promotion of sustainable development of an amaranth industry.

Description

technical field [0001] The invention belongs to the field of crop breeding, and in particular relates to a method for treating amaranth seeds with ethyl methanesulfonate. Background technique [0002] Amaranth is a 1-year-old short-sun herb of the family Amaranthaceae. It is also a C4 plant with lush stems and leaves and high nutritional value. It is cultivated in many countries in the world, and it is also cultivated in the north and south of my country. It is one of the main vegetables in summer. With the development of society, people's demand for varieties tends to be diversified, but there are problems such as narrow genetic background and lack of breakthrough excellent germplasm resources in breeding practice. Constructing a mutant library of a certain scale and obtaining a large number of mutant materials is an effective method for cultivating new varieties and conducting genetic research. [0003] In terms of breeding new varieties of amaranth, traditionally, the me...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 胡春梅崔燕刘丹侯喜林王建军
Owner NANJING AGRICULTURAL UNIVERSITY
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