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A seedling raising method for mutagenizing sorghum seeds with ethyl methanesulfonate

A technology of ethyl methanesulfonate and sorghum, applied in botany equipment and methods, plant genetic improvement, agriculture, etc., can solve the problem of affecting mutagenesis efficiency, reducing the number of mutagenized progeny materials, and inconsistent emergence density of mutagenized seeds, etc. problems, to achieve the effect of improving the emergence rate

Active Publication Date: 2021-06-25
甘肃省农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, people often sow the EMS-treated seeds directly in the field (live broadcast), because the treated seeds are generally subject to various damages, and due to the uncontrollable influence of the soil environment and climate conditions under natural conditions, the emergence rate is generally low. Even after the emergence of seedlings, the suitable environment for its growth cannot always be satisfied, so there is a huge gap between the results obtained under laboratory conditions, which seriously affects the mutagenesis efficiency and greatly reduces the available mutagenesis The number of progeny materials; at the same time, because the concentration of the mutagen is generally selected based on the half-lethal rate, at least half of the mutagenized seeds that are directly sown into the ground cannot germinate, and due to the randomness of the mutagenesis, they are directly broadcast to the ground. The emergence density of the mutagenized seeds was very inconsistent. Some mutants occupied a large area, while others occupied a small area. The absorption of light, heat, and nutrients by a single mutagenized plant was obviously inconsistent. Inaccurate identification of individual plant traits in the mutagenized M1 generation
Moreover, some late-maturing mutagenic plants cannot mature and set seeds, and thus cannot preserve the mutagenized material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0046] (1) Preparation of seedling trays:

[0047]The seedling tray is made of polypropylene material. The seedling tray is 30cm long, 20cm wide, and 5.5cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.2cm, and the distance between the centers of the small holes is 1cm; 150ppm concentration is used 2 days before the substrate is placed on the tray. The sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.

[0048] (2) Preparation of seedling substrate:

[0049] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 100ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilize...

Embodiment 2

[0063] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0064] (1) Preparation of seedling trays:

[0065] The seedling tray is 40cm long, 30cm wide, and 6cm deep. The center of each hole is a small hole of 0.3cm, made of polystyrene material; 2 days before the substrate is placed on the tray, the seedling tray is evenly sprayed with 150ppm sodium hypochlorite. Disinfect surfaces.

[0066] (2) Preparation of seedling substrate:

[0067] The seedling-raising substrate is selected from commercially available finished seedling-raising substrate: Shandong Luyi brand seedling-raising substrate (produced by Shandong Luyi Seedling Substrate Co., Ltd.); 20 days before use, the uniformly mixed substrate is sprayed with 100ppm concentration of sodium hypochlorite for disinfection, and the substrate is constantly stirred to make All matrices were uniformly sterilized, and these sterilized matrices were then spread out ...

Embodiment 3

[0077] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0078] (1) Preparation of seedling trays:

[0079] The seedling tray is made of polypropylene material. The seedling tray is 60cm long, 40cm wide, and 8cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.3cm, and the distance between the centers of the small holes is 1cm; 2 days before the substrate is placed on the tray, use 200ppm Sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.

[0080] (2) Preparation of seedling substrate:

[0081] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 200ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilized, and then these steri...

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Abstract

The invention provides a method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, which comprises the following steps: (1) preparation of seedling raising containers; (2) preparation of seedling raising substrates: preparing seedling raising substrates and sterilizing and drying them; (3) Preparation of nursery site: choose to place in a solar glass greenhouse, spread a layer of 1‑2cm thick sand on the upper part of the ground, and the lower layer is soil; (4) sowing; (5) seedling management; (6) transplanting: seedling age 35‑ After 50 days, when the seedling height reaches more than 5 cm, the seedlings are transplanted to the field. In the present invention, by growing seedlings in a greenhouse for seeds induced by EMS, the emergence rate of the mutant offspring can be significantly improved, the early maturation of the mutant single plant can be promoted, and the number of mutant offspring materials close to the theoretical value can be obtained, which is beneficial to the M1 generation Accurate identification of mutant traits of mutagenic strains.

Description

technical field [0001] The invention relates to a method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate. Background technique [0002] Ethyl methanesulfonate (EMS) mutagenesis is the most commonly used method for plant mutation breeding and construction of mutant libraries. In the past, people often sow the EMS-treated seeds directly in the field (live broadcast), because the treated seeds are generally subject to various damages, and due to the uncontrollable influence of the soil environment and climate conditions under natural conditions, the emergence rate is generally low. Even after the emergence of seedlings, the suitable environment for its growth cannot always be satisfied, so there is a huge gap between the results obtained under laboratory conditions, which seriously affects the mutagenesis efficiency and greatly reduces the available mutagenesis The number of progeny materials; at the same time, because the concentration of the mutagen ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01G31/00
CPCA01G31/00A01H1/06
Inventor 王炜陈琛叶春雷罗俊杰葛玉彬赵瑛李静雯
Owner 甘肃省农业科学院生物技术研究所
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