A seedling raising method for mutagenizing sorghum seeds with ethyl methanesulfonate
A technology of ethyl methanesulfonate and sorghum, applied in botany equipment and methods, plant genetic improvement, agriculture, etc., can solve the problem of affecting mutagenesis efficiency, reducing the number of mutagenized progeny materials, and inconsistent emergence density of mutagenized seeds, etc. problems, to achieve the effect of improving the emergence rate
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Embodiment 1
[0045] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:
[0046] (1) Preparation of seedling trays:
[0047]The seedling tray is made of polypropylene material. The seedling tray is 30cm long, 20cm wide, and 5.5cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.2cm, and the distance between the centers of the small holes is 1cm; 150ppm concentration is used 2 days before the substrate is placed on the tray. The sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.
[0048] (2) Preparation of seedling substrate:
[0049] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 100ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilize...
Embodiment 2
[0063] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:
[0064] (1) Preparation of seedling trays:
[0065] The seedling tray is 40cm long, 30cm wide, and 6cm deep. The center of each hole is a small hole of 0.3cm, made of polystyrene material; 2 days before the substrate is placed on the tray, the seedling tray is evenly sprayed with 150ppm sodium hypochlorite. Disinfect surfaces.
[0066] (2) Preparation of seedling substrate:
[0067] The seedling-raising substrate is selected from commercially available finished seedling-raising substrate: Shandong Luyi brand seedling-raising substrate (produced by Shandong Luyi Seedling Substrate Co., Ltd.); 20 days before use, the uniformly mixed substrate is sprayed with 100ppm concentration of sodium hypochlorite for disinfection, and the substrate is constantly stirred to make All matrices were uniformly sterilized, and these sterilized matrices were then spread out ...
Embodiment 3
[0077] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:
[0078] (1) Preparation of seedling trays:
[0079] The seedling tray is made of polypropylene material. The seedling tray is 60cm long, 40cm wide, and 8cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.3cm, and the distance between the centers of the small holes is 1cm; 2 days before the substrate is placed on the tray, use 200ppm Sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.
[0080] (2) Preparation of seedling substrate:
[0081] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 200ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilized, and then these steri...
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