Method for culturing sorghum seeds induced by ethyl methanesulfonate

A technology of ethyl methanesulfonate and sorghum, which is applied in the fields of botanical equipment and methods, plant genetic improvement, soilless cultivation, etc., can solve the problem of affecting the mutagenesis efficiency, reducing the number of mutagenized progeny materials, and the emergence density of mutagenized seeds. Inconsistency and other problems, to achieve the effect of improving the emergence rate

Active Publication Date: 2019-11-01
甘肃省农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the past, people often sow the EMS-treated seeds directly in the field (live broadcast), because the treated seeds are generally subject to various damages, and due to the uncontrollable influence of the soil environment and climate conditions under natural conditions, the emergence rate is generally low. Even after the emergence of seedlings, the suitable environment for its growth cannot always be satisfied, so there is a huge gap between the results obtained under laboratory conditions, which seriously affects the mutagenesis efficiency and greatly reduces the available mutagenesis The number of progeny materials; at the same time, because the concentration of the mutagen is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0046] (1) Preparation of seedling trays:

[0047]The seedling tray is made of polypropylene material. The seedling tray is 30cm long, 20cm wide, and 5.5cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.2cm, and the distance between the centers of the small holes is 1cm; 150ppm concentration is used 2 days before the substrate is placed on the tray. The sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.

[0048] (2) Preparation of seedling substrate:

[0049] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 100ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilize...

Embodiment 2

[0063] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0064] (1) Preparation of seedling trays:

[0065] The seedling tray is 40cm long, 30cm wide, and 6cm deep. The center of each hole is a small hole of 0.3cm, made of polystyrene material; 2 days before the substrate is placed on the tray, the seedling tray is evenly sprayed with 150ppm sodium hypochlorite. Disinfect surfaces.

[0066] (2) Preparation of seedling substrate:

[0067] The seedling-raising substrate is selected from commercially available finished seedling-raising substrate: Shandong Luyi brand seedling-raising substrate (produced by Shandong Luyi Seedling Substrate Co., Ltd.); 20 days before use, the uniformly mixed substrate is sprayed with 100ppm concentration of sodium hypochlorite for disinfection, and the substrate is constantly stirred to make All matrices were uniformly sterilized, and these sterilized matrices were then spread out ...

Embodiment 3

[0077] A method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate, comprising the following steps:

[0078] (1) Preparation of seedling trays:

[0079] The seedling tray is made of polypropylene material. The seedling tray is 60cm long, 40cm wide, and 8cm deep. The bottom of the seedling tray is evenly equipped with small holes of 0.3cm, and the distance between the centers of the small holes is 1cm; 2 days before the substrate is placed on the tray, use 200ppm Sodium hypochlorite is evenly sprayed on the surface of the seedling tray for surface disinfection.

[0080] (2) Preparation of seedling substrate:

[0081] The seedling substrate is prepared by mixing peat soil, vermiculite, perlite and clean fine sand in a weight ratio of 3:1:1:1; 20 days before use, the uniformly mixed substrate is sprayed with 200ppm sodium hypochlorite for disinfection. The matrices were turned constantly so that all matrices could be evenly sterilized, and then these steri...

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PUM

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Abstract

The invention provides a method for culturing sorghum seeds induced by ethyl methanesulfonate. The method comprises the following steps of (1) preparation of a seedling culture vessel; (2) preparationof a seedling culture medium, wherein the seedling culture medium is prepared, disinfected and air-dried; (3) preparation of a seedling culture site, wherein a layer of sand with the thickness of 1-2cm is laid on the upper portion of the ground of a sunlight glass greenhouse, and the lower layer is soil; (4) sowing; (5) seedling culture management; (6) transplanting, wherein seedlings are transplanted into a field when the seedling age is 35-50 days and the seedling height is 5 cm or above. According to the method, by carrying out greenhouse seedling culture on the seeds obtained after EMS mutagenesis, the emergence rate of mutagenetic offspring can be obviously increased, the maturity of mutagenetic single plants is accelerated, a nearly theoretical number of mutagenetic offspring materials are obtained, and accurate identification of the mutation characters of M1-generation mutagenetic plants is facilitated.

Description

technical field [0001] The invention relates to a method for raising seedlings of sorghum seeds induced by ethyl methanesulfonate. Background technique [0002] Ethyl methanesulfonate (EMS) mutagenesis is the most commonly used method for plant mutation breeding and construction of mutant libraries. In the past, people often sow the EMS-treated seeds directly in the field (live broadcast), because the treated seeds are generally subject to various damages, and due to the uncontrollable influence of the soil environment and climate conditions under natural conditions, the emergence rate is generally low. Even after the emergence of seedlings, the suitable environment for its growth cannot always be satisfied, so there is a huge gap between the results obtained under laboratory conditions, which seriously affects the mutagenesis efficiency and greatly reduces the available mutagenesis The number of progeny materials; at the same time, because the concentration of the mutagen ...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01G31/00
CPCA01G31/00A01H1/06
Inventor 王炜陈琛叶春雷罗俊杰葛玉彬赵瑛李静雯
Owner 甘肃省农业科学院生物技术研究所
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