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Rapid detection method of three commonly used anesthetics in aquatic products

A detection method and an anesthetic technology, applied in the field of product safety and detection, can solve the problems of unpopularity, cumbersome operation, and high concentration of target objects, and achieve the effects of easier promotion and use, high extraction efficiency, and simple operation

Active Publication Date: 2022-04-12
FISHERIES RES INST OF FUJIAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The disadvantages of this invention lie in three aspects: (1) detection by color reaction requires a high concentration level of the target, and it is difficult to achieve high-sensitivity detection. The specific value of the sensitivity of the method is not mentioned in the patent
(3) MS222 is currently the most widely used anesthetic in the world, and this method cannot detect this compound
The deficiencies of this invention lie in two aspects: (1) the pretreatment process adopts silica gel solid phase extraction column, the operation is more loaded down with trivial details, it is difficult to realize the pretreatment of large quantities of samples in a short time
(2) Triple quadrupole gas chromatography-mass spectrometer equipment is expensive, and it is not widely equipped in scientific research and testing institutions, and the method is difficult to promote and use

Method used

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  • Rapid detection method of three commonly used anesthetics in aquatic products
  • Rapid detection method of three commonly used anesthetics in aquatic products
  • Rapid detection method of three commonly used anesthetics in aquatic products

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Embodiment 1

[0040] Example 1: Method for detecting three commonly used anesthetics in aquatic products by single quadrupole GC / MS

[0041] 1 Materials and methods

[0042] (1) Instruments and reagents

[0043] Single quadrupole gas spectrometer (Thermofisher DSQ); nitrogen blowing instrument (Tianjin Hengao HGC-24); vortex mixer (IKA MS2); centrifuge (Beijing Medical); ultrapure water device Milli-Q (Milliporeco ); electronic balance (METTLER).

[0044] Standard product: eugenol (Eugenol, CAS: 97-53-0, purity 99.5%), methyl eugenol (Methyleugenol, CAS: 93-15-2, purity 99.5%), isoeugenol (Isoeugenol, CAS: 97 -57-1, purity 98.8%), purchased from Dr.Ehrenstorfer; 3-aminobenzoic acid ethyl ester methanesulfonate (MS-222, CAS: 886-86-2, purity 98%), 3-aminobenzene Formate (Methyl-3-aminobenzoate, CAS: 4518-10-9, purity 98.5%) was purchased from Merck; acetonitrile, dichloromethane, and ethyl acetate were chromatographically pure, NaoH was analytically pure, and all water was ultrapure wate...

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Abstract

The invention discloses a rapid detection method for three commonly used anesthetics in aquatic products, belonging to the technical field of inspection. The present invention utilizes a single quadrupole gas chromatography spectrometer (GC-MS) for screening, quantification and confirmation, adopts a Thermo TG 1701MS capillary column as an analysis column, and establishes and measures eugenol in aquatic products under a positive ion-selected ion monitoring mode (SIM) (Eugenol, CAS: 97‑53‑0), Isoeugenol (Isoeugenol, CAS: 97‑57‑1), ethyl 3‑aminobenzoate methanesulfonate (MS‑222, CAS: 886‑86‑ 2) Three methods of anesthetic content. The detection limits of the method were 25.0 μg / kg for eugenol and isoeugenol, and 50.0 μg / kg for ethyl 3-aminobenzoate methanesulfonate. The detection method is simpler and quicker than the traditional instrumental analysis method, and has the characteristics of good linear correlation, high sensitivity, resistance to matrix interference, high accuracy and high stability.

Description

technical field [0001] The invention belongs to the technical field of product safety and detection, and in particular relates to a rapid detection method for three commonly used anesthetics in aquatic products. Background technique [0002] With the improvement of people's living standards, the demand for fresh aquatic products is increasing. Long-distance transport of live aquatic products can easily lead to stress reactions of live aquatic animals, bruises and a large number of deaths. Anesthesia of live aquatic animals during transportation is an effective means to improve the transportation capacity and survival rate, so the use of anesthetics in the circulation process has become the norm. Countries such as the European Union, the United States, Japan, South Korea, Australia, New Zealand, and Chile have all approved several anesthetics for use in edible aquatic products. At present, the anesthetics approved for use abroad include: 3-aminobenzoic acid ethyl methylsulf...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/72
CPCG01N30/02G01N30/06G01N30/72G01N2030/025G01N2030/062
Inventor 刘海新王丽娟余颖钱卓真姜琳琳陈宇峰罗方方汤水粉位绍红郑一玲
Owner FISHERIES RES INST OF FUJIAN
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