11 results about "Acyl coenzyme" patented technology

The invention discloses an acyl-coenzyme A oxidase mutant as well as an expression strain and application thereof. On the basis of acyl coenzyme A oxidase derived from marine microorganisms, mutation sites are designed through Saprot and scored, and mutation is introduced through overlapping extension PCR (polymerase chain reaction), so that a mutant gene is obtained. After the engineering bacteria containing the mutant plasmids are subjected to induced expression, the acyl-coenzyme A oxidase with improved stability and specific enzyme activity is obtained. Under the condition of 37 DEG C, the stability of the mutant is improved by 1.3 times. When palmitoyl coenzyme A is used as a substrate, the specific enzyme activity of the mutant is improved to 1.5 times. The mutant has application value in an in-vitro diagnostic kit for detecting free fatty acid by an enzyme method.

The invention provides soybean acyl-coenzyme A oxidase and application of a coding gene thereof in regulation and control of plant salt tolerance, and belongs to the technical field of plant breeding. The invention provides soybean acyl coenzyme A oxidase and a coding gene thereof. Experiments prove that the soybean acyl coenzyme A oxidase or the coding gene thereof can improve the salt tolerance of plants through positive regulation. Therefore, the invention provides the application of a reagent for improving soybean acyl-coenzyme A oxidase or gene expression or activity or a reagent for increasing or enhancing gene expression in increasing the salt tolerance of plants, preparing a product for increasing the salt tolerance of plants, cultivating plant varieties with salt tolerance advantages, preparing a product for cultivating plants with increased salt tolerance and breeding plants with salt tolerance.

The present invention relates to a recombinant yeast for high-level production of adipic acid, wherein the activity of endogenous acyl-CoA oxidase is weakened compared to the inherent activity, and the activity of plant-derived acyl-CoA oxidase is enhanced compared to the inherent activity. The recombinant yeast according to the present invention can produce adipic acid at a high yield compared to existing unmodified microorganisms.

The present disclosure relates to synthetic biology and, in particular the bioproduction of isoprenoids using heterologous expression of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGR) enzyme(s).

Disclosed herein are novel cells that are engineered to catabolize a reduced number of acyl-CoA and branched-chain amino acids, and methods for improved cannabinoid production using these cells.In addition, disclosed are cells that are engineered to produce rare cannabinoids, methods for producing these novel rare cannabinoids, and the novel rare cannabinoid compounds thus produced.Some aspects of the disclosure are directed to cells that are engineered to provide increased production of cannabinoids and / or their derivatives compared to control cells.

The invention relates to the technical field of biology, in particular to an enzyme related to synthesis of kaempferol-3-O-(2 '-coumaroyl)-glucoside, a coding gene and application of the enzyme, and discloses acyltransferase CsCFAT, the amino acid sequence of which is shown as SEQ ID NO.1. The acyltransferase CsCFAT effectively solves the problem that existing kaempferol-3-O-(2'-coumaroyl)-glucoside is difficult to obtain. The invention discovers and identifies that the acyltransferase CsCFAT has the function of catalyzing kaempferol-3-O-glucoside and p-coumaroyl-coenzyme A to generate a target product for the first time, and fills the blank of a specific biological catalysis tool of the compound; compared with traditional plant extraction and chemical synthesis, large-scale production of CsCFAT is achieved through prokaryotic expression, a recombinant protein band after purification is single, a single product peak can be generated through catalytic reaction, and the obtaining efficiency and purity of a target compound are greatly improved.

Target RNA molecules are labeled through biotin, and accurate tracking and efficient detection of RNA can be achieved. The ribozyme capable of transferring biotin acyl to the 5'terminal of RNA (Ribose Nucleic Acid) is obtained by screening by utilizing an in-vitro screening technology and taking small molecule 12: 0 biotin acyl coenzyme A as a substrate. Then, a conservative motif of the ribozyme is obtained through screening optimization, and a biotin modification site of the ribozyme is determined. The ribozyme is designed into enzyme chain RNA with trans-activity, the biotin modification activity of the ribozyme to substrate chain RNA is verified through EMSA, urea denatured polyacrylamide gel electrophoresis and mass spectrum identification methods, and meanwhile, the transfer site and the molecular weight are determined. The ribozyme obtained by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

Disclosed herein is a method for predicting prognosis of a subject being with a cancer, such as a malignant glioma, being treated with an oncolytic herpes simplex virus (oHSV) that involves assaying a sample from the subject for Acyl-CoA-binding protein (ACBP) levels, wherein an elevated level of ACBP compared to a control is an indication of a poor prognosis. Also disclosed herein is a method for treating a cancer, such as a malignant glioma, in a subject that involves administering to the subject an effective amount of an oncolytic herpes simplex virus (oHSV) in combination with a glioma Bmi1 inhibitor or a fatty acid oxidation (FAO) inhibitor optionally followed by one or more immune checkpoint inhibitors.

The invention provides engineering modified saccharopolyspora spinosa and a method for producing spinosad by using the engineering modified saccharopolyspora spinosa. The saccharopolyspora spinosa overexpresses g6pdh-1. The content of NADPH (nicotinamide adenine dinucleotide phosphate) in the modified saccharopolyspora spinosa is increased, and the content of acyl coenzyme is increased, so that the synthesis of a lactone ring is improved, and finally, the increase of the yield of spinosad is realized. The transformed saccharopolyspora spinosa can greatly improve the yield of spinosad, and is of great significance to industrial production of spinosad.

A use of a substance for inhibiting SOAT1 gene expression and / or protein activity. The use is selected from at least one of: (a) Preparation of kits for liver cancer diagnosis; (b) Preparation of kits for liver cancer prognosis; (c) Preparation of companion diagnostic kits for treatment of liver cancer; (d) For the preparation of drugs for the prevention and / or treatment of cancer; (e) For the preparation of drugs for the prevention and / treatment of cancer spread and metastasis; (f) For the preparation of drugs that promote the apoptosis of cancer cells; (g) For the preparation of drugs for inhibiting cancer cell formation; (h) For the preparation of drugs that inhibit the proliferation and growth of cancer cells in vitro. Experiments have shown that SOAT1 is highly expressed in liver cancer tissues and serum, and its high abundance indicates poor prognosis of liver cancer patients.

The present invention relates to a method for the production of atactic poly(3-hydroxybutyrate) (PHB) comprising (a) producing a mixture of (R)-3-hydroxybutryl-CoA and (S)-3-hydroxybutryl-CoA by (a1) reducing acetoacetyl-CoA to a mixture of (R)-3-hydroxybutyryl-CoA and (S)-3-hydroxybutryl-CoA by contacting acetoacetyl-CoA with an enzyme catalyzing the reaction (R)-3-hydroxyacyl-CoA + NAD(P)+ = 3-oxoacyl-CoA + H+ + NAD(P)H and an enzyme catalyzing the reaction (3S)-3-hydroxybutyryl-CoA + NAD(P)+ = acetoacetyl-CoA + H+ + NAD(P)H in the presence of the coenzyme nicotinamide adenine dinucleotide phosphate (NADPH) or nicotinamide adenine dinucleotide (NADH), and / or (a2) the isomerization of (R)-3-hydroxybutyryl-CoA to (S)-3-hydroxybutyryl-CoA by contacting (R)-3- hydroxybutyryl-CoA with an enzyme catalyzing the isomerization of (R)-3-hydroxybutyryl-CoA to (S)-3-hydroxybutyryl-CoA, and / or (a3) the isomerization of (R)-3-hydroxybutyryl-CoA to (S)-3- hydroxybutyryl-CoA by contacting (R)-3-hydroxybutyryl-CoA with an enzyme catalyzing the reaction (3R)-3-hydroxybutyryl-CoA ↔ crotonyl-CoA + H2O to produce Crotonyl-CoA and contacting Crotonyl-CoA with an enzyme catalyzing the reversible hydration of crotonyl-CoA, and (b) polymerizing the mixture of (a) to atactic poly(3-hydroxybutyrate) by contacting the mixture of (a) with an enzyme catalyzing the reaction -3-hydroxybutyryl-CoA + poly(3-hydroxybutyrate)(n) = poly(3- hydroxybutyrate)(n+1) + CoA.