85 results about "Bioproduction" patented technology

Methods and systems for monitoring and / or controlling production units used at various stages of biological product development and manufacturing are presented herein. In particular, in certain embodiments, the biological production monitoring and control techniques described herein utilize a mid-infrared (mid-IR) analyzer that is capable of obtaining mid-infrared spectral data from an aqueous sample in substantially real time. The techniques described herein can utilize this mid-infrared spectroscopic data to measure sample quality metrics, such as protein content, titer, secondary structure, aggregation, and the like, as well as viral and / or nucleic acid characteristics. Thus, sample quality metrics can be measured substantially in real time and / or continuously to assess, for example, the production quality of protein and gene therapeutic agents, and can be used by a control system to adjust and / or control process parameters to improve product quality, etc.

To provide a novel stereoselective method for producing a cyclic dinucleotide derivative that can be used for an antibody-immunostimulator conjugate, and a production intermediate thereof, and to provide methods for producing a cyclic dinucleotide-linker and antibody-immunostimulator conjugate using the production method.SOLUTION: The present invention relates to a method that makes it possible to produce, in large amounts and with high yield, a cyclic dinucleotide derivative having a desired steric configuration, by using an optically active phosphitylation agent twice, during coupling and cyclization.SELECTED DRAWING: None

The invention is related to materials and methods for production of tetrahydrocurcumin and tetrahydrodemethoxycurcumin from substrates. The invention provides methods and materials for cell-free and host cell-based bioproduction of tetrahydrocurcumin and tetrahydrodemethoxycurcumin.

A nonviral gene therapy delivers DNA that allows cells to produce proteins such as their own growth factors in culture media. Lipid nanoparticles provide an effective delivery mechanism into a target cell. By encapsulating plasmid DNA encoding the growth factors with inducible promoters into lipid nanoparticles (LNPs), target cells create their own growth factors reducing a need for expensive growth factors from external sources. DNA expression lasts for several days reducing the amount of reagent needed. The plasmid DNA is more stable than mRNA and proteins, therefore reducing logistical challenges.

The present disclosure relates to recombinant production reporter cells comprising a logic gate such that an expressible sequence (e.g., a reporter gene) is expressed after the occurrence of triggering event related to a stage of production or an environmental condition of a biological production system. Nucleic acids, kits, and methods for making and using the recombinant production reporter cells are also disclosed herein.

Guide bearing with multiple pressure measurement points, System for measuring the viscosity or the Reynolds number of a fluid, with a rod guided by the bearing, Application to monitoring in bio-production. A guide bearing of central axis (X), including a ring, intended to be mounted tightly in a holding structure, the inner surface of which is designed for assembling with a clearance fit, and preferably guiding in translation along the axis X, a tube intended to constitute a measurement rod; an angulation element formed integrally or fixed inside the inner surface of the ring by protruding into it, so as to form an angulation axis of the tube relative to the axis X; a plurality of pressure sensors each designed to measure a point of pressure exerted by a contact point of the tube in one of its angular positions, the pressure sensors being distributed along the inner surface of the ring, on either side of the angulation element.

Recombinant microorganism in which a gene encoding a transcriptional repressor of the glycine cleavage system, pyruvate formate lyase or phosphoglycerate dehydrogenase, is attenuated or deleted from a host microorganism with a formic acid assimilation pathway, a ppsR gene encoding a phosphoenolpyruvate synthase regulatory protein, or a purT gene encoding a phosphoribosylglycinamide formyltransferase, is attenuated or deleted, a gcvTHP gene, consisting of the gcvT gene, the gcvH gene and the gcvP gene, which encodes an enzyme involved in a glycine cleavage system reaction in which host microorganism with the formic acid assimilation pathway is highly expressed, and a gene encoding formate tetrahydrofolate ligase, methenyl tetrahydrofolate cyclohydrolase or methylene tetrahydrofolate dehydrogenase is introduced into the host microorganism via the formic acid assimilation pathway.

The invention relates to a method for preparing a nano metal iron oxide material from iron ion binding protein with specific binding capacity, and belongs to the technical field of genetic engineering. In order to overcome the technical problems of non-specific binding of metallothionein and iron ions and relatively low production efficiency in a traditional nano # imgabs0 # biological preparation method, the invention provides an iron ion binding protein with specific binding capacity, and a specific binding iron ion polypeptide sequence is connected on the basis of metallothionein, so that the iron ion binding protein with specific binding capacity is obtained. The specific binding amount of iron ions is increased, and the specific binding iron ion protein is used as a template to prepare the # imgabs 1 # nano material. According to the method for preparing the nano-metal iron oxide material through specific binding of the iron ion protein, reaction conditions are mild, # imgabs2 # is prepared and synthesized in an oriented mode through high selectivity of the specific binding iron ion protein, the preparation process is easy to control, and the particle size range of the prepared # imgabs3 # is 20-1000 nm.

The invention relates to the technical field of biology, in particular to a biological preparation method of a remegapam intermediate, which comprises the following steps: by taking remegapam intermediate precursor ketone with a certain concentration as a substrate, adding recombinant engineering bacteria, reacting in a reaction system with the pH value of 8.0-9.0 at the temperature of 30-40 DEG C, and obtaining a product after the reaction is completed, wherein the recombinant engineering bacterium is an engineering bacterium containing a ketoreductase mutant coding gene, and the amino acid sequence of the ketoreductase mutant is as shown in SEQ ID NO: 4, SEQ ID NO: 19 or SEQ ID NO: 39. According to the biological preparation method of the remegapam intermediate disclosed by the embodiment of the invention, the stereoselectivity of asymmetric reduction catalysis of the used ketoreductase mutant on precursor ketone of the remegapam intermediate is improved, the R-type remegapam intermediate with high chiral purity is obtained, and the synthesis efficiency of the remegapam intermediate is favorably improved.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.

As one aspect, the present disclosure provides a bacterium of the genus Corynebacterium capable of producing isopropanol with high productivity. As another aspect, the present disclosure provides a method which enables the production of isopropanol with high productivity while suppressing the production of by-products.　In one aspect, the present disclosure relates to a bacterium of the genus Corynebacterium capable of producing isopropanol, the bacterium being produced by introducing into a bacterium of the genus Corynebacterium a specific gene (A) that encodes an enzyme having an activity of producing acetoacetic acid from acetoacetyl-CoA and / or a specific gene (B) that encodes an enzyme having an activity of producing acetone from acetoacetic acid in a manner that enables expression thereof. In another aspect, the present disclosure relates to a method for producing isopropanol, the method comprising: allowing a genetically modified bacterium of the genus Corynebacterium capable of producing isopropanol to carry out a fermentation reaction in a fermentation tank to produce a fermentation product containing isopropanol; continuously or intermittently withdrawing a portion of a reaction solution containing the fermentation product from the fermentation tank during the fermentation reaction; separating isopropanol from the withdrawn reaction solution by pervaporation; and supplying to the fermentation tank the reaction solution from which isopropanol has been separated.

The present invention relates to: a mutant microorganism having improved L-citrulline or L-arginine productivity; and a method for producing L-citrulline or L-arginine using same. The mutant microorganism can improve the production yield of L-citrulline or L-arginine compared to the parent strain by weakening or inactivating the activity of a protein encoded by the NCgl2653 gene.

The present invention belongs to the field of industrial biotechnology, and discloses a method for improving 1-deoxynojirimycin bioproduction strains, ybbA gene and application, the method comprising introducing the ybbA gene, an expression cassette comprising the ybbA gene or a vector comprising the ybbA gene into Bacillus amyloliquefaciens. The present invention uses precise gene knockout and backfilling technology to conduct a comprehensive functional analysis of the ybbA gene in Bacillus amyloliquefaciens. It aims to clarify the exact role of the ybbA gene in DNJ biosynthesis and provide a scientific basis and theoretical support for the development of novel and efficient DNJ production strategies. The research results of the present invention are expected to break through the bottleneck of DNJ yield improvement and open up new paths and methods for related research in the field of industrial biotechnology.

The present invention provides a mammalian cell line that has been genetically engineered to make it resistant to viral entry and / or replication; and provides a method for using the cell line to reduce or prevent viral contamination of a bioproduction system.

Methods and systems for cell-free bioproduction of retinal and retinol are provided, which may be performed using a combination of a beta-carotene 15,15'-dioxygenase and an alcohol dehydrogenase. Cell-free retinoid production enables reaction rates and productivity not possible / not obtained in cell-based systems.

The present invention relates to compositions and methods for the production of UDP-sugars, including UDP-glucose and UDP-galactose. In particular, the present invention provides engineered cell lines for the production of UDP-sugars, and methods for the production of UDP-sugars using these engineered cell lines. Also provided herein are engineered cells for the production of UDP-glucose, wherein the engineered cells comprise one or more genetic modifications that increase UDP-glucose production by increasing the metabolic flux to the UDP-glucose precursor and / or by reducing carbon losses resulting from the production of by-products.

The present invention discloses a nano-liposome loaded with fish collagen peptides, its preparation method and application, belonging to the fields of polypeptide and bioproduction processing. In the present invention, fish collagen peptide solid powder is dissolved in pure water, and further, soybean lecithin and phytosterol are added to the collagen polypeptide solution. The above substances are fully mixed by high-speed homogenization and whipping, and then the emulsion is introduced into a microfluidic homogenizer for high-pressure homogenization. Subsequently, the emulsion is diluted and added to tangential flow ultrafiltration, and diluted and ultrafiltered repeatedly for multiple times to separate the liposome from the free peptide, obtain high-purity liposomes, and at the same time realize the recovery and reuse of the free peptide. Compared with the current mainstream preparation methods, its preparation method has the advantages of not introducing organic solvents, simplifying the process, recycling the unentrapped raw materials, and reducing raw material waste.

Real-time quality control of a culture for bioproduction is facilitated using machine learning. In this approach, real-time process data for a set of parameters for a current production run is received. Based on this process data, a prediction is made using an instance of a machine learning model that has been trained on process data from past production or development runs. The instance is uniquely associated to a particular culture day and thus independent of any other instance of the machine learning model (for other culture days). Based on the prediction, a quality control recommendation for the current production run is then made. Several different types of predictions are enabled, and various different recommendations are provided based on the predictions.

It provides a bioengineered bacteriophage-lime nanoparticle, rQβ@b-3WJ, comprising a broccoli light-up aptamer including 3WJ RNA scaffold (b-3WJ) integrated with a nucleic acid bioproduction and self-packaging system to produce b-3WJ packaged Qβ VLPs. Also provided is a nanoparticle, TrQβ@b-3WJ, which is the TrQβ@b-3WJ with the conjugation of TAT peptide on the surface, which enhance cellular internalization for highly efficient gene silencing.

The invention is directed to materials and methods for bioproduction of pectolinarigenin. The invention provides methods and materials for cell-based and cell-free production of pectolinarigenin.

The utility model discloses a preparation device of yeast lysate, which belongs to the technical field of microbial production and comprises culture equipment. Collecting equipment; the heating equipment comprises a heating cylinder, a material pipe and a heating resistance wire, the material pipe and the heating resistance wire are installed in the heating cylinder, the material pipe is attached to the heating resistance wire, the material pipe and the heating resistance wire are made of heat conduction materials, the material pipe and the heating resistance wire are spiral, and the upper end of the material pipe is connected with the collecting equipment through a pipeline; crushing equipment; a filtering device; according to the utility model, the heating equipment is arranged, so that fermentation liquor enters the upper end part of the material pipe through the pipeline after being subjected to centrifugal separation treatment; under the action of gravity, the fermentation liquor gradually falls down, the heating resistance wire emits heat, and the heat is transferred to the fermentation liquor in the material pipe through the separation barrel and the material pipe; through heating, the structure of yeast cell walls is changed, the mechanical strength of the cell walls is reduced, and the subsequent crushing effect is better.

Disclosed herein are nanorod productions systems (NPS) useful for the production of biological scalable functionalization-ready nanorods (BSFnano). The nanorods produced are derived from filamentous phage Ff (f1, M13 or fd). The NPS disclosed herein permits efficient biological production of non-infectious, heat-stable isomorphic proteinaceous nanorods comprising modifications allowing site-specific recombinant, chemical and enzymatic attachment of peptide and non-peptide functionalities in an orthogonal manner. Also disclosed are methods of making and using these nanorods, such as in methods of detecting target molecules.

Methods and systems for performing a bioproduction process are described, the bioproduction process comprising the production of a cellular product by a cell population. The methods include the steps of culturing the cell population at least partially embedded in a hydrogel matrix forming a three-dimensional structure.

The present invention relates to a method for detecting a host cell protein (HCP) native to a host cell species, in a composition of proteins produced by said host cell species and comprising HCPs and at least one biological entity that is not native to the host cell species, wherein the method comprises a dual-recognition immunoassay. The invention also relates to methods for assessing quality and reproducibility of aspects of biological production processes, as well as kits and use of kits in such methods.

This utility model relates to the field of experimental biological production equipment technology, and discloses a biological experimental cabinet, including a cabinet body. A hydraulic cylinder is fixedly installed on the top of the operating table inside the cabinet body. An installation plate is fixedly installed on the output shaft of each hydraulic cylinder. A round rod is fixedly installed on the outer wall of the installation plate. A rotating drawer is rotatably installed on the outer wall of the round rod. A partition is installed on the inner wall of the rotating drawer. A magnet is provided on the bottom inner wall of the rotating drawer. This utility model balances structural strength and hydraulic cylinder installation requirements through a stepped design of the installation plate. The height can be adjusted by the hydraulic cylinder to move the rotating drawer, facilitating cleaning of the operating table and freeing up desktop space to accommodate different experimental equipment. The inner partition of the rotating drawer uses a magnetic adsorption design, allowing for quick assembly and adjustment of the spatial layout to meet diverse experimental needs. The shelves are slidably engaged with slots and protrusions, making installation and disassembly simple and easy to clean.

The present invention relates to a recombinant microorganism capable of producing polyhydroxyalkanoate and a method for producing polyhydroxyalkanoate, using same and, more specifically, to: a recombinant microorganism in which a gene encoding an enzyme for converting amino acids into 2-hydroxy acids, a gene encoding hydroxyisocaproate-CoA transferase, and a gene encoding polyhydroxyalkanoate synthase are introduced or amplified; and a method for producing polyhydroxyalkanoate, using the recombinant microorganism.

A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.

The invention provides a technical method for producing high-purity linalool based on a microbial fermentation method, which is characterized in that a linalool synthase gene is modified and edited based on an AlphaFold gene through sgRNA design and carrier construction, strain transformation and gene cleavage verification, glucose is combined as a core carbon source, and a yeast extract and peptone are selected as nitrogen sources, so that the high-purity linalool is produced. The method comprises the following steps of: preparing a basic culture medium, subculturing to obtain a target strain, improving the linalool synthase performance, realizing the iterative optimization of the efficient strain, preparing and sterilizing a fermentation culture medium, inoculating the strain, starting fermentation, monitoring and regulating fermentation parameters in real time, automatically adjusting the parameters and the like to realize a fermentation reaction to obtain linalool. According to the method, the linalool synthase is taken as a raw material, key precursor substances such as acetyl coenzyme A and pentose phosphate are added, and a proper amount of inducer and cofactor are introduced, so that the linalool synthase catalytic efficiency of the strain is enhanced, the problems of low fermentation yield, high separation and purification cost, poor large-scale production stability and the like at present are effectively solved, and green biological production of high-purity linalool is realized.

The present disclosure relates to synthetic biology and, in particular the bioproduction of isoprenoids using heterologous expression of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGR) enzyme(s).

This invention discloses a bio-preparation method for a key chiral intermediate of erlotinib ethyl ester. The method utilizes resting cells of *Saccharomyces cerevisiae* ZJPH1807 as a catalyst to prepare (R)-1-[4-chloro-2-(3-methyl-1H-pyrazol-1-yl)phenyl]-2,2,2-trifluoroethanol via biocatalysis of 1-[4-chloro-2-(3-methyl-1H-pyrazol-1-yl)phenyl]-2,2,2-trifluoroethanol. The product obtained using this strain exhibits high optical purity (ee value > 99.9%) and a reaction yield of 86.4%. The process is simple, environmentally friendly, and uses microbial cells as the biocatalyst, resulting in low cost.