57 results about "Bioproduction" patented technology

To provide a novel stereoselective method for producing a cyclic dinucleotide derivative that can be used for an antibody-immunostimulator conjugate, and a production intermediate thereof, and to provide methods for producing a cyclic dinucleotide-linker and antibody-immunostimulator conjugate using the production method.SOLUTION: The present invention relates to a method that makes it possible to produce, in large amounts and with high yield, a cyclic dinucleotide derivative having a desired steric configuration, by using an optically active phosphitylation agent twice, during coupling and cyclization.SELECTED DRAWING: None

The invention is related to materials and methods for production of tetrahydrocurcumin and tetrahydrodemethoxycurcumin from substrates. The invention provides methods and materials for cell-free and host cell-based bioproduction of tetrahydrocurcumin and tetrahydrodemethoxycurcumin.

A nonviral gene therapy delivers DNA that allows cells to produce proteins such as their own growth factors in culture media. Lipid nanoparticles provide an effective delivery mechanism into a target cell. By encapsulating plasmid DNA encoding the growth factors with inducible promoters into lipid nanoparticles (LNPs), target cells create their own growth factors reducing a need for expensive growth factors from external sources. DNA expression lasts for several days reducing the amount of reagent needed. The plasmid DNA is more stable than mRNA and proteins, therefore reducing logistical challenges.

The present disclosure relates to recombinant production reporter cells comprising a logic gate such that an expressible sequence (e.g., a reporter gene) is expressed after the occurrence of triggering event related to a stage of production or an environmental condition of a biological production system. Nucleic acids, kits, and methods for making and using the recombinant production reporter cells are also disclosed herein.

Recombinant microorganism in which a gene encoding a transcriptional repressor of the glycine cleavage system, pyruvate formate lyase or phosphoglycerate dehydrogenase, is attenuated or deleted from a host microorganism with a formic acid assimilation pathway, a ppsR gene encoding a phosphoenolpyruvate synthase regulatory protein, or a purT gene encoding a phosphoribosylglycinamide formyltransferase, is attenuated or deleted, a gcvTHP gene, consisting of the gcvT gene, the gcvH gene and the gcvP gene, which encodes an enzyme involved in a glycine cleavage system reaction in which host microorganism with the formic acid assimilation pathway is highly expressed, and a gene encoding formate tetrahydrofolate ligase, methenyl tetrahydrofolate cyclohydrolase or methylene tetrahydrofolate dehydrogenase is introduced into the host microorganism via the formic acid assimilation pathway.

The invention relates to the technical field of biology, in particular to a biological preparation method of a remegapam intermediate, which comprises the following steps: by taking remegapam intermediate precursor ketone with a certain concentration as a substrate, adding recombinant engineering bacteria, reacting in a reaction system with the pH value of 8.0-9.0 at the temperature of 30-40 DEG C, and obtaining a product after the reaction is completed, wherein the recombinant engineering bacterium is an engineering bacterium containing a ketoreductase mutant coding gene, and the amino acid sequence of the ketoreductase mutant is as shown in SEQ ID NO: 4, SEQ ID NO: 19 or SEQ ID NO: 39. According to the biological preparation method of the remegapam intermediate disclosed by the embodiment of the invention, the stereoselectivity of asymmetric reduction catalysis of the used ketoreductase mutant on precursor ketone of the remegapam intermediate is improved, the R-type remegapam intermediate with high chiral purity is obtained, and the synthesis efficiency of the remegapam intermediate is favorably improved.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.

As one aspect, the present disclosure provides a bacterium of the genus Corynebacterium capable of producing isopropanol with high productivity. As another aspect, the present disclosure provides a method which enables the production of isopropanol with high productivity while suppressing the production of by-products.　In one aspect, the present disclosure relates to a bacterium of the genus Corynebacterium capable of producing isopropanol, the bacterium being produced by introducing into a bacterium of the genus Corynebacterium a specific gene (A) that encodes an enzyme having an activity of producing acetoacetic acid from acetoacetyl-CoA and / or a specific gene (B) that encodes an enzyme having an activity of producing acetone from acetoacetic acid in a manner that enables expression thereof. In another aspect, the present disclosure relates to a method for producing isopropanol, the method comprising: allowing a genetically modified bacterium of the genus Corynebacterium capable of producing isopropanol to carry out a fermentation reaction in a fermentation tank to produce a fermentation product containing isopropanol; continuously or intermittently withdrawing a portion of a reaction solution containing the fermentation product from the fermentation tank during the fermentation reaction; separating isopropanol from the withdrawn reaction solution by pervaporation; and supplying to the fermentation tank the reaction solution from which isopropanol has been separated.

The present invention provides a mammalian cell line that has been genetically engineered to make it resistant to viral entry and / or replication; and provides a method for using the cell line to reduce or prevent viral contamination of a bioproduction system.

Methods and systems for cell-free bioproduction of retinal and retinol are provided, which may be performed using a combination of a beta-carotene 15,15'-dioxygenase and an alcohol dehydrogenase. Cell-free retinoid production enables reaction rates and productivity not possible / not obtained in cell-based systems.

It provides a bioengineered bacteriophage-lime nanoparticle, rQβ@b-3WJ, comprising a broccoli light-up aptamer including 3WJ RNA scaffold (b-3WJ) integrated with a nucleic acid bioproduction and self-packaging system to produce b-3WJ packaged Qβ VLPs. Also provided is a nanoparticle, TrQβ@b-3WJ, which is the TrQβ@b-3WJ with the conjugation of TAT peptide on the surface, which enhance cellular internalization for highly efficient gene silencing.

The utility model discloses a preparation device of yeast lysate, which belongs to the technical field of microbial production and comprises culture equipment. Collecting equipment; the heating equipment comprises a heating cylinder, a material pipe and a heating resistance wire, the material pipe and the heating resistance wire are installed in the heating cylinder, the material pipe is attached to the heating resistance wire, the material pipe and the heating resistance wire are made of heat conduction materials, the material pipe and the heating resistance wire are spiral, and the upper end of the material pipe is connected with the collecting equipment through a pipeline; crushing equipment; a filtering device; according to the utility model, the heating equipment is arranged, so that fermentation liquor enters the upper end part of the material pipe through the pipeline after being subjected to centrifugal separation treatment; under the action of gravity, the fermentation liquor gradually falls down, the heating resistance wire emits heat, and the heat is transferred to the fermentation liquor in the material pipe through the separation barrel and the material pipe; through heating, the structure of yeast cell walls is changed, the mechanical strength of the cell walls is reduced, and the subsequent crushing effect is better.

Disclosed herein are nanorod productions systems (NPS) useful for the production of biological scalable functionalization-ready nanorods (BSFnano). The nanorods produced are derived from filamentous phage Ff (f1, M13 or fd). The NPS disclosed herein permits efficient biological production of non-infectious, heat-stable isomorphic proteinaceous nanorods comprising modifications allowing site-specific recombinant, chemical and enzymatic attachment of peptide and non-peptide functionalities in an orthogonal manner. Also disclosed are methods of making and using these nanorods, such as in methods of detecting target molecules.

The present invention relates to a method for detecting a host cell protein (HCP) native to a host cell species, in a composition of proteins produced by said host cell species and comprising HCPs and at least one biological entity that is not native to the host cell species, wherein the method comprises a dual-recognition immunoassay. The invention also relates to methods for assessing quality and reproducibility of aspects of biological production processes, as well as kits and use of kits in such methods.

This utility model relates to the field of experimental biological production equipment technology, and discloses a biological experimental cabinet, including a cabinet body. A hydraulic cylinder is fixedly installed on the top of the operating table inside the cabinet body. An installation plate is fixedly installed on the output shaft of each hydraulic cylinder. A round rod is fixedly installed on the outer wall of the installation plate. A rotating drawer is rotatably installed on the outer wall of the round rod. A partition is installed on the inner wall of the rotating drawer. A magnet is provided on the bottom inner wall of the rotating drawer. This utility model balances structural strength and hydraulic cylinder installation requirements through a stepped design of the installation plate. The height can be adjusted by the hydraulic cylinder to move the rotating drawer, facilitating cleaning of the operating table and freeing up desktop space to accommodate different experimental equipment. The inner partition of the rotating drawer uses a magnetic adsorption design, allowing for quick assembly and adjustment of the spatial layout to meet diverse experimental needs. The shelves are slidably engaged with slots and protrusions, making installation and disassembly simple and easy to clean.

A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.

The invention provides a technical method for producing high-purity linalool based on a microbial fermentation method, which is characterized in that a linalool synthase gene is modified and edited based on an AlphaFold gene through sgRNA design and carrier construction, strain transformation and gene cleavage verification, glucose is combined as a core carbon source, and a yeast extract and peptone are selected as nitrogen sources, so that the high-purity linalool is produced. The method comprises the following steps of: preparing a basic culture medium, subculturing to obtain a target strain, improving the linalool synthase performance, realizing the iterative optimization of the efficient strain, preparing and sterilizing a fermentation culture medium, inoculating the strain, starting fermentation, monitoring and regulating fermentation parameters in real time, automatically adjusting the parameters and the like to realize a fermentation reaction to obtain linalool. According to the method, the linalool synthase is taken as a raw material, key precursor substances such as acetyl coenzyme A and pentose phosphate are added, and a proper amount of inducer and cofactor are introduced, so that the linalool synthase catalytic efficiency of the strain is enhanced, the problems of low fermentation yield, high separation and purification cost, poor large-scale production stability and the like at present are effectively solved, and green biological production of high-purity linalool is realized.

The present disclosure relates to synthetic biology and, in particular the bioproduction of isoprenoids using heterologous expression of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGR) enzyme(s).

This invention discloses a bio-preparation method for a key chiral intermediate of erlotinib ethyl ester. The method utilizes resting cells of *Saccharomyces cerevisiae* ZJPH1807 as a catalyst to prepare (R)-1-[4-chloro-2-(3-methyl-1H-pyrazol-1-yl)phenyl]-2,2,2-trifluoroethanol via biocatalysis of 1-[4-chloro-2-(3-methyl-1H-pyrazol-1-yl)phenyl]-2,2,2-trifluoroethanol. The product obtained using this strain exhibits high optical purity (ee value > 99.9%) and a reaction yield of 86.4%. The process is simple, environmentally friendly, and uses microbial cells as the biocatalyst, resulting in low cost.

In various embodiments, provided herein are host cells, methods, and pharmaceutical compositions, including complex sphingolipids, such as monosialic acid ganglioside 1 (GM1), monosialic acid ganglioside 2 (GM2), and monosialic acid ganglioside 3 (GM3). In some embodiments, the present disclosure provides biological production of glycosphingolipids. In still further embodiments, the present disclosure provides for the biological production of gangliosides.

In various embodiments, host cells, methods, and pharmaceutical compositions comprising complex sphingolipids such as monosialoganglioside 1 (GM1), monosialoganglioside 2 (GM2), and monosialoganglioside 3 (GM3) are provided herein. In some embodiments, the disclosure provides the bioproduction of sphingoglycolipids. In yet another embodiment, the disclosure provides the bioproduction of gangliosides.

The application discloses Halomonas for producing PHA by using CO2 electrochemical derivatives, a construction method and application of the Halomonas, and the Halomonas expresses Rhs644 genes and Rhs647 genes from Halomonas sp.TD01. The application realizes production of poly-3-hydroxybutyrate by using CO2 electrochemical derivatives as a single carbon source in the Halomonas, without additional adding of antibiotics and inducers, greatly reduces production cost, and improves single batch fermentation income. Meanwhile, efficient utilization of a cheap carbon source is realized, and the process of low-carbon biological manufacturing can be promoted. The poly-3-hydroxybutyrate is produced by using the application, and the time period is short, the production process is simple, the environment is green and pollution-free, and the application is beneficial to industrialized large-scale production.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

This invention provides a titer method using UV measurement for continuous biological production. [Solution] This specification discloses a method for determining the loading volume of capture chromatography in a system and method for the continuous production of biological products.

Methods for monitoring a polypeptide type concentration comprise obtaining a first signal from first measurements from a first substrate-based sensor exposed to a biologic sample. The first signal includes a resonance contribution for each first measurement, for each of a first plurality of polypeptide types, based on polypeptide type concentration in the sample and first substrate binding affinity for the polypeptide type. A second signal is obtained from second measurements from a second substrate-based sensor exposed to the sample. The second signal includes a resonance contribution for each second measurement, for each of a second plurality of polypeptide types, based on polypeptide type concentration in the sample and second substrate binding affinity for the polypeptide type. The first and second substrate binding affinity for a first polypeptide type in biologic sample differ. The first polypeptide type concentration is determined using the first and second signals.

A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.

The present invention concerns a metabolically engineered microorganism for glycine bioproduction or a salt or an ester thereof, the genome of said microorganism comprises an attenuation of the expression of genes encoding enzymes having glycine cleavage system activity as defined by E.C. 1.4.1.27 together with an overexpressing of threonine dehydrogenase dependent pathway as defined by EC E.C. 1.1.1.103 and E.C. 2.3.1.29 and / or with a threonine aldolase dependent pathway as defined by E.C. 4.1.2.48 or EC 4.1.2.42 or any of its catalytically active variants, its use for the production of glycine or one of its salts or esters. The present invention also concerns a fermentation process using said metabolically engineered microorganism for the production of glycine or one of its salts or esters.

An enzymatic process and system are disclosed for producing homogeneous chain length poly hydroxyalkanoate monomers and / or polymers having a chain length of at least eight carbons from a long-chain fatty acid or precursor thereof. The process also results in specific homogenous chain length polyhydroxyalkanoate monomers and / or polymers. The long-chain fatty acid or precursor thereof can be contacted in vitro with an enzyme or a mixture of enzymes to produce the homogeneous chain length polyhydroxyalkanoate.

A method for mixing solutions includes receiving a flowing first solution containing a biopharmaceutical and directing the flowing first solution along a flow path including a dilution position where a dilution device is in fluid communication with the flow path; introducing a second solution into the flowing first solution at the dilution position to form a flowing third solution; measuring the biopharmaceutical in the flowing first solution at a position upstream from the dilution position; measuring the biopharmaceutical in the flowing third solution at a position downstream from the dilution position; determining a relative relationship between the measurements of the biopharmaceutical or values ​​derived from the measurements of the biopharmaceutical at the upstream and downstream positions; and adjusting the flow rate of at least one of the first and second solutions based on the relative relationship.

Electrochemical measurement device for a fluid incorporating at least one electrochemical probe, in particular at the free end of a pole, and a protective element rotating around the probe, Application to monitoring in bioproduction.The invention relates to an electrochemical measuring device for a non-gaseous fluid, in particular contained in a container, such as a tank, especially one with opaque walls, comprising: - a measuring pole in the form of a tube (2) with longitudinal axis X, one free end of which is intended to be immersed in the fluid; - at least one electrochemical probe, fixed to the free end of the measuring pole, and adapted to measure the electrochemical properties of the fluid; - an element (3) mounted for rotation around the tube and comprising at least one wall with a plurality of open holes (31 to 39) shaped such that the peripheral surface (300) of each hole is formed so that a flow of the fluid over the wall causes an asymmetry of the bearing forces on each peripheral surface of the hole and thus sets the element in rotation about the axis X. Figure for the abstract: Fig. 1.