36 results about "Bioproduction" patented technology

The invention is related to materials and methods for production of tetrahydrocurcumin and tetrahydrodemethoxycurcumin from substrates. The invention provides methods and materials for cell-free and host cell-based bioproduction of tetrahydrocurcumin and tetrahydrodemethoxycurcumin.

A nonviral gene therapy delivers DNA that allows cells to produce proteins such as their own growth factors in culture media. Lipid nanoparticles provide an effective delivery mechanism into a target cell. By encapsulating plasmid DNA encoding the growth factors with inducible promoters into lipid nanoparticles (LNPs), target cells create their own growth factors reducing a need for expensive growth factors from external sources. DNA expression lasts for several days reducing the amount of reagent needed. The plasmid DNA is more stable than mRNA and proteins, therefore reducing logistical challenges.

The present disclosure relates to recombinant production reporter cells comprising a logic gate such that an expressible sequence (e.g., a reporter gene) is expressed after the occurrence of triggering event related to a stage of production or an environmental condition of a biological production system. Nucleic acids, kits, and methods for making and using the recombinant production reporter cells are also disclosed herein.

Recombinant microorganism in which a gene encoding a transcriptional repressor of the glycine cleavage system, pyruvate formate lyase or phosphoglycerate dehydrogenase, is attenuated or deleted from a host microorganism with a formic acid assimilation pathway, a ppsR gene encoding a phosphoenolpyruvate synthase regulatory protein, or a purT gene encoding a phosphoribosylglycinamide formyltransferase, is attenuated or deleted, a gcvTHP gene, consisting of the gcvT gene, the gcvH gene and the gcvP gene, which encodes an enzyme involved in a glycine cleavage system reaction in which host microorganism with the formic acid assimilation pathway is highly expressed, and a gene encoding formate tetrahydrofolate ligase, methenyl tetrahydrofolate cyclohydrolase or methylene tetrahydrofolate dehydrogenase is introduced into the host microorganism via the formic acid assimilation pathway.

The invention relates to the technical field of biology, in particular to a biological preparation method of a remegapam intermediate, which comprises the following steps: by taking remegapam intermediate precursor ketone with a certain concentration as a substrate, adding recombinant engineering bacteria, reacting in a reaction system with the pH value of 8.0-9.0 at the temperature of 30-40 DEG C, and obtaining a product after the reaction is completed, wherein the recombinant engineering bacterium is an engineering bacterium containing a ketoreductase mutant coding gene, and the amino acid sequence of the ketoreductase mutant is as shown in SEQ ID NO: 4, SEQ ID NO: 19 or SEQ ID NO: 39. According to the biological preparation method of the remegapam intermediate disclosed by the embodiment of the invention, the stereoselectivity of asymmetric reduction catalysis of the used ketoreductase mutant on precursor ketone of the remegapam intermediate is improved, the R-type remegapam intermediate with high chiral purity is obtained, and the synthesis efficiency of the remegapam intermediate is favorably improved.

The present invention relates to a Corynebacterium sp. mutant microorganism producing L-glutamic acid and a method of producing L-glutamic acid using the same, and more specifically, to a novel NADP-dependent malic enzyme variant involved in the L-glutamic acid biosynthetic pathway, a polynucleotide, and a transformant, as well as a method of producing L-glutamic acid using the same. The NADP-dependent malic enzyme variant according to the present invention is obtained by substituting one or more amino acids in the amino acid sequence constituting NADP-dependent malic enzyme to change the enzymatic activity of the NADP-dependent malic enzyme, and a recombinant microorganism comprising the NADP-dependent malic enzyme variant is capable of efficiently producing L-glutamic acid.

As one aspect, the present disclosure provides a bacterium of the genus Corynebacterium capable of producing isopropanol with high productivity. As another aspect, the present disclosure provides a method which enables the production of isopropanol with high productivity while suppressing the production of by-products.　In one aspect, the present disclosure relates to a bacterium of the genus Corynebacterium capable of producing isopropanol, the bacterium being produced by introducing into a bacterium of the genus Corynebacterium a specific gene (A) that encodes an enzyme having an activity of producing acetoacetic acid from acetoacetyl-CoA and / or a specific gene (B) that encodes an enzyme having an activity of producing acetone from acetoacetic acid in a manner that enables expression thereof. In another aspect, the present disclosure relates to a method for producing isopropanol, the method comprising: allowing a genetically modified bacterium of the genus Corynebacterium capable of producing isopropanol to carry out a fermentation reaction in a fermentation tank to produce a fermentation product containing isopropanol; continuously or intermittently withdrawing a portion of a reaction solution containing the fermentation product from the fermentation tank during the fermentation reaction; separating isopropanol from the withdrawn reaction solution by pervaporation; and supplying to the fermentation tank the reaction solution from which isopropanol has been separated.

The present invention provides a mammalian cell line that has been genetically engineered to make it resistant to viral entry and / or replication; and provides a method for using the cell line to reduce or prevent viral contamination of a bioproduction system.

Methods and systems for cell-free bioproduction of retinal and retinol are provided, which may be performed using a combination of a beta-carotene 15,15'-dioxygenase and an alcohol dehydrogenase. Cell-free retinoid production enables reaction rates and productivity not possible / not obtained in cell-based systems.

The present invention relates to a method for detecting a host cell protein (HCP) native to a host cell species, in a composition of proteins produced by said host cell species and comprising HCPs and at least one biological entity that is not native to the host cell species, wherein the method comprises a dual-recognition immunoassay. The invention also relates to methods for assessing quality and reproducibility of aspects of biological production processes, as well as kits and use of kits in such methods.

This utility model relates to the field of experimental biological production equipment technology, and discloses a biological experimental cabinet, including a cabinet body. A hydraulic cylinder is fixedly installed on the top of the operating table inside the cabinet body. An installation plate is fixedly installed on the output shaft of each hydraulic cylinder. A round rod is fixedly installed on the outer wall of the installation plate. A rotating drawer is rotatably installed on the outer wall of the round rod. A partition is installed on the inner wall of the rotating drawer. A magnet is provided on the bottom inner wall of the rotating drawer. This utility model balances structural strength and hydraulic cylinder installation requirements through a stepped design of the installation plate. The height can be adjusted by the hydraulic cylinder to move the rotating drawer, facilitating cleaning of the operating table and freeing up desktop space to accommodate different experimental equipment. The inner partition of the rotating drawer uses a magnetic adsorption design, allowing for quick assembly and adjustment of the spatial layout to meet diverse experimental needs. The shelves are slidably engaged with slots and protrusions, making installation and disassembly simple and easy to clean.

A bioproduction mixing system includes a flexible compartment having a first end, a second end, and a sidewall extending therebetween, a center axis passing through the flexible compartment between the first end and the second end. A helical assembly is disposed within the flexible compartment and is suspended between the first end and the second end, the helical assembly being offset from the center axis of the flexible compartment.

The present disclosure relates to synthetic biology and, in particular the bioproduction of isoprenoids using heterologous expression of 3-hydroxy-3-methylglutaryl-coenzyme-A reductase (HMGR) enzyme(s).

In various embodiments, host cells, methods, and pharmaceutical compositions comprising complex sphingolipids such as monosialoganglioside 1 (GM1), monosialoganglioside 2 (GM2), and monosialoganglioside 3 (GM3) are provided herein. In some embodiments, the disclosure provides the bioproduction of sphingoglycolipids. In yet another embodiment, the disclosure provides the bioproduction of gangliosides.

Embodiments of the present invention provides recombinant systems, methods, and compositions for the biological production of Testosteronan (Testan), a linear α-(1→4)-linked glucuronic acid–N-acetylglucosamine polysaccharide. Microbial hosts engineered to express full-length CtTS, N-terminal truncation variants (including d64-CtTS), or the Pseudomonas CIPTS homolog synthesize high–molecular-weight Testan (≥800 kDa) with low polydispersity. Structural analysis by ¹H NMR and 2D HSQC demonstrates that all recombinant enzymes produce Testan that is chemically indistinguishable from native polymer. Purification using ultrafiltration and strong-anion exchange chromatography yields highly defined molecular-weight fractions, including monodisperse populations (PDI ~1.02). The invention further provides sulfated derivatives of Testan produced via aqueous sulfation methods, as well as Testan and functionalized Testan compositions useful for chromatographic stationary phases, biomaterial scaffolds, and chemically modifiable polymer platforms. These systems provide the first scalable, fermentation-based production methods for Testan and Testan-derived materials.

This invention provides a titer method using UV measurement for continuous biological production. [Solution] This specification discloses a method for determining the loading volume of capture chromatography in a system and method for the continuous production of biological products.

An enzymatic process and system are disclosed for producing homogeneous chain length poly hydroxyalkanoate monomers and / or polymers having a chain length of at least eight carbons from a long-chain fatty acid or precursor thereof. The process also results in specific homogenous chain length polyhydroxyalkanoate monomers and / or polymers. The long-chain fatty acid or precursor thereof can be contacted in vitro with an enzyme or a mixture of enzymes to produce the homogeneous chain length polyhydroxyalkanoate.

Electrochemical measurement device for a fluid incorporating at least one electrochemical probe, in particular at the free end of a pole, and a protective element rotating around the probe, Application to monitoring in bioproduction.The invention relates to an electrochemical measuring device for a non-gaseous fluid, in particular contained in a container, such as a tank, especially one with opaque walls, comprising: - a measuring pole in the form of a tube (2) with longitudinal axis X, one free end of which is intended to be immersed in the fluid; - at least one electrochemical probe, fixed to the free end of the measuring pole, and adapted to measure the electrochemical properties of the fluid; - an element (3) mounted for rotation around the tube and comprising at least one wall with a plurality of open holes (31 to 39) shaped such that the peripheral surface (300) of each hole is formed so that a flow of the fluid over the wall causes an asymmetry of the bearing forces on each peripheral surface of the hole and thus sets the element in rotation about the axis X. Figure for the abstract: Fig. 1.

The present disclosure relates generally to the field of bio-production of low-molecular weight hyaluronic acids. The present disclosure also relates to compositions comprising said hyaluronic acid, and the various uses thereof.

In some other conventional carminic acid production methods, carminic acid production may be carried out within a host organism and / or cells. The present invention relates to materials and methods for the production of carminic acid from substrates. The present invention provides methods and materials for cell-free bioproduction of carminic acid. The methods provided in the present invention relate to cell-free production of carminic acid that is more economical and reliable compared to other conventional carminic acid production methods. Furthermore, the methods provided in the present invention provide carminic acid from these processes that offers higher potency compared to carminic acid produced by other conventional methods.

Provided herein are compositions and methods for engineering a bacterium comprising a reduced capacity for cellulose formation, a reduced capacity for foaming behavior, or an increased capacity for cell density for enhancing bioprocessing outcomes associated with the fermentative production of industrially relevant bioproducts.

Provided herein are compositions and methods for engineering a bacterium comprising a reduced capacity for cellulose formation, a reduced formation, a reduced capacity for foaming behavior, or an increased capacity for cell density for enhancing bioprocessing outcomes associated with the fermentative production of industrially relevant bioproducts.

The present disclosure relates to recombinant engineered polypeptides with cytochrome P450 (CYP) activity capable of selectively catalyzing the conversion of precursor compounds, carvacrol and / or thymol, to the product compound, thymohydroquinone (THQ), and recombinant host cells that heterologously express genes encoding these polypeptides (e.g., in yeast) and that can be used for enhanced fermentative bioproduction of the product compound THQ.

The present invention relates to a recombinant microorganism having the ability to produce a polyhydroxyalkanoate (PHA) copolymer, and a method for producing a PHA copolymer using same. More particularly, the present invention relates to a recombinant microorganism having the ability to produce a PHA copolymer containing 3-hydroxyalkanoate (3-HHx) as a monomer, in which a gene encoding a PHA synthase derived from an Aeromonas sp. strain is introduced or amplified, and to a method for producing a PHA copolymer using same.

The present invention relates to a mutant microorganism exhibiting enhanced 1,4-butanediol production capability and a method for producing 1,4-butanediol by using same, wherein attenuation of serine / threonine protein kinase activity in the mutant microorganism enhances the flux of the 1,4-butanediol biosynthetic pathway, thereby enabling efficient production of 1,4-butanediol.

Disclosed herein are methods for harvesting an antibody as a recombinant protein comprising an asparaginyl endopeptidase (AEP, legumain) cleavage site containing the amino acids NF, by maintaining the pH after fermentation at 4.6 to 5.3 for acidification to precipitate impurities in order to prevent the self-cleavage of proenzyme and thus the activation of peptidase at lower pH, as well as methods for inhibiting the cleavage of a recombinant protein comprising an AEP cleavage site during a bioproduction process.

The invention is related to materials and methods for production of kermesic acid and / or carminic acid from substrates. The invention provides methods and materials for cell-free and host cell-based bioproduction of kermesic acid and / or carminic acid.

Device for measuring the flow velocity of a fluid, System integrating at least one electrochemical probe and the measuring device, in particular at the free end of a pole, Application to monitoring in bioproduction. The invention relates to a device (1) for measuring the flow velocity of a non-gaseous fluid, in particular contained in a container, such as a tank, particularly one with opaque walls, comprising: - a tube (2) with longitudinal axis X; - an element (3) mounted for rotation around the tube and comprising at least one wall with a plurality of open holes (31 to 39) shaped such that the peripheral surface (300) of each hole is formed so that a flow of the fluid over the wall causes an asymmetry of the bearing forces on each peripheral surface of the hole and thus sets the element in rotation around the axis X; - a rotary encoder (5) adapted to measure the rotation of the element and thereby the flow velocity of the fluid.Figure for the abridged version: Fig.1.

The present invention discloses a bacterium and an obtaining method and application thereof. The bacterium has a property of coproducing 1,3-propanediol and D-lactic acid. Further, the bacterium is Klebsiella oxytoca, including Klebsiella oxytoca PDL-5 CCTCC M 2016185. The obtaining method of the bacterium may be to obtain the bacterium by directly screening wild bacteria that satisfy conditions from the environment or performing gene engineering modification to wild bacteria. The present invention has the advantages that the bacteria can coproduce 1,3-propanediol and D-lactic acid through fermentation, the molar conversion rate and the concentration of the two products are very high, the types of byproducts are few, the concentration is low, the product extraction process is simplified, the high-efficiency biological production of 1,3-propanediol and D-lactic acid can be realized, and the industrial application prospect is very great.

A consortium of engineered microorganisms for producing tryptophan-derived products and methods of using the same.