Building method of library for detecting non-small cell lung cancer gene mutation and kit

Abstract

The invention discloses a building method of a library for detecting non-small cell lung cancer gene mutation and a kit. The method includes: using tubular reaction to complete genome DNA breaking and connector connection, performing hybrid capture on connection products after amplification and non-small cell lung cancer related gene target area probes, and performing BGISEQ-500 / 1000 platform sequencing and data analysis to obtain mutation conditions. The method has the advantages that the experiment flow is optimized greatly by the tubular reaction, operation complexity and time are reduced, and the requirements on clinical sample initial amount are lowered; multiple genes and multiple sites can be detected in one step, point mutation, insertion and deletion, structural variation and copy number variation are covered, the detecting result is accurate and overcomes the defect that a PCR capture method cannot detect the structural variation in one step, and the effectiveness of the high-throughput sequencing applied to the detection of the non-small cell lung cancer gene mutation; the method is wide in coverage, high in cost performance, capable of providing a reference basis for the diagnosing, treatment and drug use performed by doctors, and the method is suitable for being popularized and used in a large-scale manner.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to the technical field of sequencing library construction, in particular to a library construction method and kit for detecting non-small cell lung cancer gene mutations. Background technique [0002] At present, lung cancer is one of the most common malignant tumors in the world, and has become the first cause of death of malignant tumors in urban population in my country, and about 85% of lung cancers are non-small cell lung cancers. Conventional chemotherapy or radiotherapy will seriously affect the quality of life and physical fitness of patients, so more and more attention has been paid to targeted therapy. For non-small cell lung cancer, existing scientific research and clinical trials have identified a series of targeted treatment sites and drugs. Different from traditional chemotherapy, targeted therapy does not interfere with all continuously dividing cells, but ...

AI Technical Summary

Problems solved by technology

It solves the problem that the current clinical method cannot rely on a small amount of DNA samples to comprehensively detect point mutations, indels, fusion genes and copy number variations at one time, and greatly saves the demand for precious clinical samples

Images