Composition for improving premature ovarian failure, preparation method therefor and application of composition

Abstract

The invention belongs to the technical field of biomedicines and particularly relates to a composition for improving premature ovarian failure, a preparation method therefor and application of the composition. According to the composition for improving the premature ovarian failure, provided by the invention, an in-vitro-constructed human uterine endometrium stem-cell cell membrane and a mesenchymal stem cell exosome are used in a compounded manner, thus, the development of ovarian follicles can be promoted, ovaries can be promoted to secrete an anti-Mullerian hormone, an ovarian follicle generator promoting level can be regulated, the apoptosis of ovarian cells can be inhibited, the adverse reserve function disease of the ovaries can be improved, the effect of improving primary or secondary premature ovarian failure is good, and thus, a new way of think is provided for clinically treating ovarian insufficiency and polycystic ovarian syndrome.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a composition for improving premature ovarian failure, a preparation method and application thereof. Background technique [0002] Premature ovarian failure refers to the premature decline of ovarian function in women. It is divided into primary premature ovarian failure and secondary premature ovarian failure. It is specifically manifested in some symptoms of ovarian function decline, such as menstrual cycle disorders, abnormal menstrual flow, and even infertility. Existing treatment methods include drugs, surgery, stem cells and stem cell derivatives, tissue engineering methods, etc. Drug treatment mainly consists of taking Chinese and Western medicines. Chinese medicines are taken for a long time, the effect is not obvious, and it is easy to cause liver and kidney toxicity. Western medicines are mainly hormone drugs, but taking hormones for a long time has side...

AI Technical Summary

Problems solved by technology

Drug treatment mainly consists of taking Chinese and Western medicines. Chinese medicines are taken for a long time, the effect is not obvious, and it is easy to cause liver and kidney toxicity. Western medicines are mainly hormone drugs, but taking hormones for a long time has side effects and cannot completely restore ovarian function; surgical treatment mainly For malnutrition-induced premature ovarian failure caused by vascular factors, intra-ovarian vascular bypass surgery is required, with high risk and strong pain for patients; stem cell and stem cell derivative therapy is currently an emerging therapy, including mesenchymal stem cell suspension injection therapy and Stem Cell Exosome Therapy

Although both mesenchymal stem cell suspension therapy and exosome therapy have made some progress, the cells are prone to absorption and diffusion after injection of single cell suspension, and cannot achieve uniform distribution, resulting in low repair efficiency, while exosomes with tissue The microenvironment changes, so the range of treatment is narrow, suitable for primary premature ovarian failure

The tissue engineering method is to combine seed cells and exogenous scaffold materials to form a biologically active tissue substitute, but the seed cells must be collected after trypsin digestion, which destroys the original extracellular matrix and intercellular junction proteins, affecting The biological activity and function of cells, and so far, there is no fully qualified scaffold material, and most of the collagen scaffold materials currently used are of animal origin, and there are immune risks

[0003] In summary, the problem in the prior art is that there is a lack of a drug for the treatment of premature ovarian failure with few side effects, a wide range of treatment and high safety.

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