11 results about "Human endometrium" patented technology

The invention relates to the technical field of medical gel, and particularly discloses a sustained-release gel preparation for endometrial repair and a preparation method of the sustained-release gel preparation. Keratinocyte factor sustained-release microspheres containing keratinocyte factor-glucan particles and lactic acid-glycolic acid copolymer shells are constructed around keratinocyte factors, and the keratinocyte factor sustained-release microspheres are loaded by hyaluronic acid hydrogel containing polylysine, collagen and silk fibroin to form the sustained-release gel preparation for endometrial repair. The sustained-release gel preparation has the effect of promoting proliferation of human endometrial cells, can repair endometrial injury, promote injury healing and prevent uterine cavity adhesion, and has important significance in treating endometrial injury and improving physical and psychological health of women.

The invention relates to the technical field of biology, and particularly discloses a construction method and application of an endometrial epithelium organ fibrosis model. According to the method, human endometrial epithelial cells are extracted, and an endometrial epithelial organ is established by adopting a Matrigel three-dimensional culture system. Afterwards, the organoid is induced to form endometrial phenotypes in different physiological cycles through hormone stimulation, and a ferroptosis inducer FIN56 is added in the culture process to induce fibrosis. The expression of GPX4, Fibronectin and p-Smad2 / 3 is detected through Western blot and immunofluorescence, and the successful construction of the fibrosis model is verified. The invention provides an in-vitro model capable of simulating endometrial fibrosis pathological characteristics of a patient with PCOS (polycystic ovarian syndrome), the in-vitro model can be used for mechanism research and drug screening of PCOS-related endometrial lesions, and a new research tool and an intervention strategy are provided for treatment of PCOS endometrial fibrosis.

The invention provides application of injectable bioactive hydrogel in treatment of recurrent abortion, and relates to the technical field of biomedicine, and the technical key points are as follows: aldehydic hyaluronic acid and chitosan are connected in parallel, the injectable bioactive hydrogel is developed, and the hydrogel is loaded with LIF, VEGF, IL-11 and valproic acid to enhance regeneration activity. According to the application, the effect is evaluated in vitro through a specific verification experiment. Results show that the hydrogel prepared by the application shows rapid gelation, good biocompatibility and factor loading capacity, and enhances the proliferation of human endometrial stromal cells and the vascularization capacity of human umbilical vein endothelial cells. In macrophages, the expression of a proinflammatory cytokine is down-regulated, and the expression of an anti-inflammatory cytokine IL-10 is up-regulated. In conclusion, the HA-CHO / chitosan hydrogel in the application supports endometrial regeneration by synergistically promoting proliferation, angiogenesis, immune regulation and receptivity.

The present invention belongs to the technical field of animal model construction, and particularly relates to a method for constructing a spontaneous endometrial cancer mouse model and its application. The construction method includes constructing a mouse model with the genotype Cre; PTEN<supgt;flox / ‑< / supgt>; STK11<supgt;flox / flox< / supgt> through the Cre / loxP system. After activating the expression of Cre recombinase in uterine epithelial cells of this mouse model, the PTEN gene and the STK11 gene are selectively knocked out, thereby spontaneously inducing the occurrence of endometrial cancer. By selectively deleting the PTEN gene and the STK11 gene in uterine epithelial cells, the present invention rapidly changes the uterine pathology, and has the advantages of short tumorigenesis time and high tumorigenesis rate. Moreover, due to the use of in-situ tumorigenesis technology, the mouse model of the present invention can simulate the growth environment of human endometrial cancer, and the histological characteristics of the tumor are extremely close to those of human endometrial cancer, meeting the biological characteristics of human endometrial cancer.

The application discloses an osmium complex, a preparation method thereof and application of the osmium complex in preparation of a medicine for treating endometriosis. The complex has a near-infrared light catalytic treatment effect on human endometrial ectopic cells (12Z), can generate superoxide anions and singlet oxygen under irradiation of a 700 nm light source, and has catalytic oxidation ability on NADH / NADPH in the 12Z cells. The series of complexes have good proliferation inhibition ability (IC 50 <40 μM) on the 12Z cells, and the proliferation inhibition ability on the 12Z cells is enhanced with optimization of a ligand structure, which has important significance for research on a new medicine for treating endometriosis and provides a new idea for developing a photodynamic treatment medicine for endometriosis in a clinic.

The invention discloses a culture medium for promoting rapid proliferation of human endometrial mesenchymal stem cells and a preparation method of the culture medium. The culture medium comprises a serum-free basic culture medium, a solvent and the following components according to the final volume of the culture medium: 0.7-1.3 mM of aesculin, 0.7-1.3 mM of apigenin, 0.7-1.3 mM of baicalin and 7-13 mM of vanillin. The culture medium provided by the invention optimizes proliferation of human endometrial mesenchymal stem cells in primary culture and early culture.

The invention provides a pharmaceutical preparation for repairing endometrial injury, and belongs to the technical field of biomedicine. The pharmaceutical preparation takes improved polypeptide as an active ingredient, and the amino acid sequence of the improved polypeptide is shown as SEQ ID NO.5. Experimental results show that the polypeptide can significantly promote proliferation and migration of human endometrial stromal cells, still maintains low cytotoxicity under high concentration, and shows excellent safety. Furthermore, in a rat endometrial mechanical injury model, the preparation can obviously increase the endometrial thickness, improve the Ki-67 positive cell rate, enhance the proliferation activity of endometrial cells and promote the repair of damaged tissues. Compared with the original polypeptide, the preparation disclosed by the invention shows unexpected technical effects in the aspects of repairing activity and safety, and has a good application prospect.

The present invention provides the use of a composition containing cryopreserved PRP in the treatment of gynecological diseases. It is also provided that the reproductive promoting peptide can significantly promote the proliferation and migration activities of human endometrial stromal cells, and can reduce the apoptosis level of cells, showing a good promoting effect. In addition, the present invention has also prepared cryopreserved PRP, and after combining it with the reproductive promoting peptide, it can significantly promote the repair of rat endometrial injury, showing a good therapeutic effect.

The present invention relates to the use of a reagent for detecting the methylation level of a target region in the preparation of a diagnostic product for endometrial cancer; the target region includes region 1 or a partial region of region 1, and / or region 2 or a partial region of region 2; with GRCh38.p14 as the reference, region 1 is the positive strand of Chr6:27679636-27680274, and region 2 is the positive strand of Chr6:10390489-10390886. In this application, these regions are used as the target regions, and by detecting the methylation level of these target regions or their partial regions, healthy people, patients with benign endometrial lesions, and patients with endometrial cancer can be effectively distinguished. Moreover, the detection rate of this application for other uterine malignancies (such as cervical cancer) is not high. It is applicable to cervical exfoliated cell samples, can achieve simple, rapid, minimally invasive detection of endometrial cancer, with high sensitivity and strong specificity.

The invention provides a construction method of a human endometrial organ aging model, and belongs to the technical field of biological models. On the basis of a human endometrial organ, hydrogen peroxide (H2O2) is utilized to stimulate and induce to obtain the human endometrial organ aging model. The human endometrial organoid senescence model can clearly present various cell senescence phenotypes such as slow down of organoid growth speed, reduction of cell proliferation index (Ki67), enhancement of SA-beta-gal activity, up-regulation of expression of cell cycle inhibition proteins P16 and P21, significant up-regulation of gene expression level of senescence-related secretion phenotype (SASP) components, and the like. And an effective tool can be provided for female anti-aging drug screening, high-throughput and high-content screening, individualized treatment strategy development and mechanism research.