Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Regenerated human alkali fiber-cell growth factor gel former and preparing method

A technology of fibroblasts and growth factors, which is applied in the direction of medical preparations containing active ingredients, drug combinations, and pharmaceutical formulas. Painful, faster healing, easy to wash off

Inactive Publication Date: 2006-08-09
北京双鹭立生医药科技有限公司
View PDF0 Cites 19 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, during the use of existing freeze-dried powders and sprays, it is necessary to add a dissolving solution to the freeze-dried powder for dissolving before use. The method of use is very troublesome, and after being applied to the affected area, the drug activity remains for a short time, which is limited in clinical application.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Take 1g of carbomer, 2g of glycerin, 1g of ethyl p-hydroxybenzoate, and 1g of glycine. The above raw materials will become acidic after mixing. To ensure protein invariance, add 0.1g of sodium hydroxide, adjust the pH value, and add to dissolve Water to 100g, mix well, then sterilize bacteria under high pressure at 0.1MPa, 121℃ for 25 minutes, cool to room temperature, make the matrix part, add rh-bFGF 300μg to the matrix, and slowly stir evenly to make a gel , And then aliquoted into aluminum tubes to make finished pharmaceutical rh-bFGF gels.

[0025] The verification experiment and related experiments involved in Example 1 of the present invention include:

[0026] 1. Stability test: The activity is measured by BalB / c 3T3 cells and MTT method. This product is sensitive to heat and should not be stored at high temperature; it can be stored at room temperature for about 3 months, which can meet the requirements of use. Under the condition of 4~8℃, the original a...

Embodiment 2

[0034] Example 2: Take 2g of sodium carboxymethyl cellulose, 5g of glycerol, 3g of methyl p-hydroxybenzoate, and Tween-800.1g, add dissolved water to 100g, mix well, and then under the conditions of 0.1MPa and 121°C , Autoclave for 25 minutes, cool to room temperature, make the matrix part. Add 300μg of rh-bFGF into the matrix and stir it evenly at the same time to form a gel, and then divide it into aluminum tubes to make a finished product of medicinal rh-bFGF gel. Prepare rh-bFGF gel.

Embodiment 3

[0035]Example 3: Take 5g of polyethylene glycol, 5g of propylene glycol, 3g of sorbic acid, 3g of Tween, add dissolved water to 100g, mix well, and then autoclave at 0.1MPa, 121°C for 25 minutes, and cool to room temperature , Make the matrix part. Add 300μg of rh-bFGF to the matrix while stirring slowly to form a gel, and then divide it into aluminum tubes to make a finished product of medicinal rh-bFGF gel.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a rh-bFGF gel and the making method that is a drug used to cure wound, canker, scald, and burn. It is compounded by 146 amino acids. The main drug is rh-bFGF, and gel basal material includes thickener, humectant, antiseptic, stabilizer, pH regulator, and water. The manufacture method includes the following steps: mixing the thickener, humectant, antiseptic, stabilizer, pH regulator, and water according to certain ratio, whisking to equal to make water solubility gel basal material, adding rh-bFGF after high pressure sterilization, mixing equally to make gel and separately packaging into aluminum mold tube to make drug using rh-bFGF gel products.

Description

Technical field [0001] The invention relates to a gel for the treatment of wounds, surgical injuries, burns, scalds and ulcers, etc., specifically a mixture of recombinant human basic fibroblast growth factor (hereinafter referred to as rh-bFGF) and a suitable matrix The present invention also relates to a preparation method of the gel for external use. Background technique [0002] Basic fibroblast growth factor (bFGF) is an endogenous active factor closely related to the healing process of tissue wounds. It has a significant promoting effect on tissue wound healing, nerve injury repair and angiogenesis. bFGF is a single-chain polypeptide composed of 146 amino acids with a molecular weight of 17KD. The company directly cloned human bFGF cDNA from Chinese peripheral blood leukocytes, and realized the gene cloning and expression of bFGF, successfully developed recombinant human bFGF expression products, and declared rh-bFGF products as tissue wound healing drugs in 1996. In March ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K38/18A61K9/00A61P17/02
Inventor 徐明波王勇波陈献华陈遥郝艳君李学海
Owner 北京双鹭立生医药科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com