The invention provides an acanthopanan trifoliatus extract, and a preparation method and an application thereof. According to the invention, acanthopanan trifoliatus entire plant is weighed and is soaked by using ethanol; rotary evaporation concentration is carried out, and a water solution is obtained; the water solution is sequentially extracted by using petroleum ether, ethyl acetate, and n-butanol; and an obtained extraction liquid is subjected to reduced-pressure distillation, such that a paste is obtained, wherein the paste comprises petroleum ether extract, ethyl acetate extract, n-butanol extract, and water-soluble matter. Through a free radical oxidation resistance experiment, a macrophage growth experiment, a NF-kB nuclear transcription factor inhibition experiment, and an Erk1/2 activation effect experiment, it is verified that the acanthopanan trifoliatus extract has anti-oxidative and anti-inflammatory capacities. Inflammatory-treating medicines or anti-oxidative cosmetics or foodstuff compositions with added acanthopanan trifoliatus and extracts thereof are provided with anti-inflammatory and anti-oxidative effects. The acanthopanan trifoliatus extract can be used in inflammation-controlling or aging-controlling cosmetics, medicines, vegetable beverages and health-care foodstuffs.