Acanthopanan trifoliatus extract, and preparation method and application thereof

An extract and technology of bok choy, applied in the field of bok choy extract and its preparation, can solve problems such as systematic in-depth research, development and application, and achieve the effects of inhibiting activity, reducing or inhibiting inflammation, and inhibiting growth

Active Publication Date: 2013-02-13
GUANGDONG UNIV OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have found that bougainvillea leaves contain a large amount of terpenes, terpene alcohols and a small amount of long-chain aliphatic and aromatic compounds. It is speculated that it sh

Method used

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  • Acanthopanan trifoliatus extract, and preparation method and application thereof
  • Acanthopanan trifoliatus extract, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the preparation of bougainvillea extract

[0034] Extraction: first weigh 1000g of naturally dried bok choy and crush it, pass through a 60-mesh sieve, take 100g and put it in a 5L round bottom flask, add 2000mL of 70% ethanol solution, heat to boil, keep for 2h, then filter with gauze, take the filtrate, repeat 3 times; the above extract was evaporated to dryness under reduced pressure, weighed, added appropriate amount of distilled water, ultrasonically extracted, and freeze-dried to obtain the ethanol extract; add distilled water with a mass ratio of 1:10 to the ethanol extract to dissolve, and add an equal volume of petroleum Ether, shake well, let stand in the separating funnel for 1h, get petroleum ether layer and water layer, extract three times respectively, collect the petroleum ether layer extract; continue to add ethyl acetate, n-butanol successively according to the volume ratio of 1:1 Solvent extraction, after liquid separation, take the upper...

Embodiment 2

[0036] Embodiment 2: DPPH method measures the antioxidant activity of bougainvillea extract

[0037] Antioxidant activity of bougainvillea extract was determined using 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH). DPPH is a relatively stable lipid free radical with a free electron on its N. Its ethanol solution is purple and has a maximum absorption peak at 517nm. After adding bok choy extract, DPPH captures an electron and pairs with a free electron, the purple fades and becomes a colorless substance, and the absorption at 517nm disappears, and the degree of fading has a quantitative relationship with the number of electrons it accepts. Based on this principle, the spectrophotometer is used to detect the change of absorbance value after the reaction between DPPH free radicals and bougainvillea extract, which can test the ability of the sample to provide hydrogen atoms, scavenge free radicals and resist oxidation. Method: Weigh 0.02g of DPPH crystals, dissolve them in absolu...

Embodiment 3

[0040] Embodiment 3: ABTS method is measured the antioxidant activity of bougainvillea extract

[0041] The principle of the ABTS method for determining the total antioxidant capacity is that ABTS is oxidized into green ABTS+ under the action of an appropriate oxidant, and the production of ABTS+ will be inhibited in the presence of antioxidants, and the ABTS+ is measured at 734nm or 405nm Absorbance can be measured and calculated the total antioxidant capacity of the sample. Method: Weigh 0.0192g of ABTS and dissolve it in 5ml of absolute ethanol to prepare a solution with a concentration of 7mmol / L. Weigh 0.0378g of potassium persulfate powder, dissolve it in 1ml of distilled water, and prepare a solution with a concentration of 140mmol / L. Take 5ml of 7mmol / L ABTS+ solution and 88μl of 140mmol / L potassium persulfate solution, mix them, let stand for 12h, and prepare ABTS+ free radical stock solution. Store the solution under light-proof conditions. Before use, dilute it wi...

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Abstract

The invention provides an acanthopanan trifoliatus extract, and a preparation method and an application thereof. According to the invention, acanthopanan trifoliatus entire plant is weighed and is soaked by using ethanol; rotary evaporation concentration is carried out, and a water solution is obtained; the water solution is sequentially extracted by using petroleum ether, ethyl acetate, and n-butanol; and an obtained extraction liquid is subjected to reduced-pressure distillation, such that a paste is obtained, wherein the paste comprises petroleum ether extract, ethyl acetate extract, n-butanol extract, and water-soluble matter. Through a free radical oxidation resistance experiment, a macrophage growth experiment, a NF-kB nuclear transcription factor inhibition experiment, and an Erk1/2 activation effect experiment, it is verified that the acanthopanan trifoliatus extract has anti-oxidative and anti-inflammatory capacities. Inflammatory-treating medicines or anti-oxidative cosmetics or foodstuff compositions with added acanthopanan trifoliatus and extracts thereof are provided with anti-inflammatory and anti-oxidative effects. The acanthopanan trifoliatus extract can be used in inflammation-controlling or aging-controlling cosmetics, medicines, vegetable beverages and health-care foodstuffs.

Description

technical field [0001] The invention relates to a bok choy extract and its preparation method and application. Background technique [0002] Bougainvillea ( Acanthopanan trifoliatus (L.) Merr ), also known as white bougainvillea, bitter thorn, Schefflera chinensis, Sanye Wujia, Sanjiapi, is a climbing shrub of Araliaceae, mainly distributed in Yunnan, Sichuan, Guangdong and other regions of my country, from northeast India to Indochina, Japan and the Philippines are also distributed. Bougainvillea has been a local specialty with the same source of medicine and food since ancient times, and its use has a long history. Li Shizhen said: "Wujia cures rheumatism and paralysis, strengthens muscles and bones, and its merits are profound." In the "Food Materia Medica" of the Ming Dynasty, it is recorded that "five plus, the leaves are used as vegetable food, and the skin rheumatism is removed". According to the "Compendium of Medicinal Plants in Guangdong" (compiled by Wu Xiuren)...

Claims

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Application Information

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IPC IPC(8): A61K36/254A61K8/97A61P39/06A61P29/00A61Q19/08A23L1/29A23L33/00A61K8/9789
Inventor 张焜胡瑞连王华倩艾伦·康尼杜志云郑希李晨悦郑俊霞卢宇靖吴盼盼黄华容方岩雄赵肃清汪舰曾华强
Owner GUANGDONG UNIV OF TECH
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