Aspergillus oryzae and application method thereof in preparing sauce fragrant flavor material
A technology for Aspergillus oryzae and sauce flavor, which is applied in the field of Aspergillus oryzae and its application in the field of preparation of flavoring materials, can solve the problems of potential safety hazards of chemically synthesized meat flavors, insufficient product aroma, increased production costs, etc., and achieves high safety and aftertaste. Long, full-bodied effect
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Embodiment 1
[0019] (1) Take 10kg of fresh beef (without fat), grind it with a screw meat grinder, add 3kg of water, cook for 2.0h, and cool to room temperature for later use.
[0020] (2) Mix 2.0kg of defatted soybean meal with 0.5kg of bran, moisten with water (2.0kg) for 30 minutes, cook at normal pressure for 50 minutes, and cool to room temperature for later use.
[0021] (3) Aspergillus oryzae (CCTCC NO.M2012265) and Aspergillus niger ATCC16404 were successively inoculated in soybean milk slant medium to activate for 3-5 generations, and then inoculated into bran medium (water content 40- 45%), cultured in an environment at 28°C and a relative humidity of 95% for 40 hours to prepare Aspergillus oryzae and Aspergillus niger species for use.
[0022] (4) Take a certain amount of Aspergillus oryzae and Aspergillus niger seed koji and mix well with 2.5kg flour, so that the number of spores of Aspergillus oryzae and Aspergillus niger reaches 2.0×10 8 and 6.6×10 7 spores / g material.
[...
Embodiment 2
[0031] (1) Take 10kg of fresh pork (without fat), grind it with a screw meat grinder, add 3kg of water, cook for 1.5h, and cool to room temperature for later use.
[0032] (2) Mix 1.5kg of defatted soybean meal with 0.5kg of bran, moisten with water (1.6kg) for 30 minutes, cook at normal pressure for 50 minutes, and cool to room temperature for later use.
[0033] (3) Aspergillus oryzae (CCTCC NO.M2012265) and Aspergillus niger ATCC16404 were successively inoculated in soybean milk slant medium to activate for 3-5 generations, and then inoculated into bran medium (water content 40- 45%), respectively cultured at 28°C and 95% relative humidity for 40 hours to prepare Aspergillus oryzae and Aspergillus niger species for use.
[0034] (4) Take a certain amount of Aspergillus oryzae and Aspergillus niger seed koji and mix well with 2.5kg flour, so that the number of spores of Aspergillus oryzae and Aspergillus niger reaches 1.0×10 8 and 3.3×10 7 spores / g material.
[0035] (5) ...
Embodiment 3
[0043] (1) Take 10kg of fresh chicken breast, grind it with a screw meat grinder, add 3kg of water, cook for 1.0h, and cool to room temperature for later use.
[0044] (2) The rest of the steps are the same as in Example 2, and finally the seasoning of chicken with strong sauce flavor is worth it.
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