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Culture method of Aspergillus niger seeds

A culture method and a technology for Aspergillus niger spores are applied in the field of biological fermentation, which can solve the problems that the growth stage cannot be guaranteed, it is difficult to truly reflect the growth state of Aspergillus niger, and the fermentation results are affected, and the invention achieves good timeliness, simple and convenient measurement, and consumption. low effect

Active Publication Date: 2016-05-04
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is difficult to truly reflect the growth state of Aspergillus niger, and due to the differences between batches, the cultivation time is used to determine the transplantation, resulting in different growth stages of seeds between different batches, and it cannot guarantee that they are in a state with high vigor. Growth stage, thus affecting the subsequent fermentation results

Method used

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  • Culture method of Aspergillus niger seeds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] After the corn is crushed, pass through an 80-mesh sieve; the obtained corn flour and tap water are mixed evenly at a material-to-water ratio of 1:3, the slurry pH is adjusted to 5.8, and the high temperature α-amylase is added at the addition amount of 20U / g corn flour; The obtained slurry was sprayed twice, and a qualified liquefied liquid was obtained after the iodine test was light brown.

[0034] The liquefied liquid is diluted with tap water, and ammonium sulfate is added to prepare a seed culture medium with a total sugar of 10% and a total nitrogen of 0.2%. After sterilizing at 121°C for 20 minutes and cooling to 37°C, insert the Aspergillus niger CICC40021 spore suspension (strain source: CICC preserved strain) to make the concentration of spores 300,000 / ml after inoculation.

[0035] The seed culture conditions are: the temperature is 37℃, the dissolved oxygen concentration and the stirring speed are coupled with feedback control, and the dissolved oxygen solubilit...

Embodiment 2

[0038] After the corn is crushed, pass through an 80-mesh sieve; the obtained corn flour and tap water are mixed evenly at a material-to-water ratio of 1:3, the slurry pH is adjusted to 5.8, and the high temperature α-amylase is added at the addition amount of 20U / g corn flour; The obtained slurry was sprayed twice, and a qualified liquefied liquid was obtained after the iodine test was light brown.

[0039] The liquefied liquid is diluted with tap water, and ammonium sulfate is added to prepare a seed culture medium with a total sugar of 10% and a total nitrogen of 0.2%. After sterilizing at 121°C for 20 minutes and cooling to 37°C, insert the Aspergillus niger CICC40021 spore suspension (strain source: CICC preserved strain) to make the concentration of spores 300,000 / ml after inoculation.

[0040] The seed culture conditions are: the temperature is 37℃, the dissolved oxygen concentration and the air volume are coupled with feedback control, and the dissolved oxygen solubility is...

Embodiment 3

[0043] After the corn is crushed, pass through an 80-mesh sieve; the obtained corn flour and tap water are mixed evenly at a material-to-water ratio of 1:3, the slurry pH is adjusted to 5.8, and the high temperature α-amylase is added at the addition amount of 20U / g corn flour; The obtained slurry was sprayed twice, and a qualified liquefied liquid was obtained after the iodine test was light brown.

[0044] The liquefied liquid is diluted with tap water, and ammonium sulfate is added to prepare a seed culture medium with a total sugar of 12% and a total nitrogen of 0.4%. After sterilization at 121°C for 20 minutes and then cooling to 38°C, the Aspergillus niger CICC40021 spore suspension (strain source: CICC preserved strain) was inserted to make the concentration of spores 400,000 / ml after inoculation.

[0045] The seed culture conditions are: temperature 38°C, dissolved oxygen concentration and stirring speed coupled feedback control, by adjusting the stirring speed to control t...

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Abstract

The invention provides a culture method of Aspergillus niger seeds. The transplantation opportunity is selected by monitoring the acid production speed, the glucose consumption speed or the glucoamylase activity in the culture process. The invention specifically discloses a method for determining the acid production speed, the glucose consumption speed or the glucoamylase activity and discloses influence of the concentration of dissolved oxygen on the culture process. The defect that the growth state of the seeds is judged mainly by observing the sizes and shapes of Aspergillus niger balls in a conventional culture method is overcome. The standard deviation of fermentation results of the seeds cultured with the method is smaller, the seed fermentation in adjacent batches is more stable, the fermentation conversion rate is increased, and the fermentation period is remarkably shortened.

Description

Technical field [0001] The invention belongs to the technical field of biological fermentation, and specifically relates to a seed culture method of Aspergillus niger. Background technique [0002] Citric acid is currently the organic acid with the largest output and consumption, and is widely used in beverage, food, and pharmaceutical industries. my country is a major producer of citric acid, with a total production capacity of over 1 million tons. Domestic citric acid production uses Aspergillus niger as a strain and uses corn, cassava and other starches as raw materials for submerged liquid fermentation, generally using secondary fermentation. Before fermentation to produce citric acid, primary seed culture is usually carried out to obtain a large amount of Aspergillus niger mycelium with high vigor and strong acid production ability. [0003] During the growth of microorganisms, the cell viability is usually higher in the middle and late stages of logarithmic growth. Generall...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12Q3/00C12P7/48C12R1/685
CPCC12N1/14C12P7/48C12Q3/00
Inventor 石贵阳陈坚胡志杰李江华蒋小东彭艳红金赛孙福新王宝石王莉
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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