Mechanized production process for multi-strain yellow wine raw wheat koji
A technology of raw wheat koji and multi-strain, applied in the field of brewing, can solve the problems of unstable production quality and single strain, and achieve the effects of saving dosage, labor and easy cultivation
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Embodiment 1
[0029] Embodiment 1: the raw wheat koji automatic production method of the multi-bacteria rice wine of artificial inoculation:
[0030] Wort solid medium and bran medium were prepared according to the method described in the summary of the invention.
[0031] Aspergillus niger CCTCCM2015202 and Aspergillus oryzae CCTCCM2015201 were inoculated in eggplant bottle wort solid medium respectively, and cultured at 27°C for 6 days. Wash the spores separately with sterile water to make a spore suspension.
[0032] The spore suspensions of Aspergillus niger and Aspergillus oryzae were respectively inoculated into the bran medium of the Erlenmeyer flask, cultured at 27°C for 6 days, and shaken once every 24h.
[0033] The first-grade bran seeds of Aspergillus niger and Aspergillus oryzae were inoculated into the bran culture medium of the koji culture machine (ZQB-50BJ type koji culture machine) respectively according to the inoculation amount of 1%. The controlled temperature was 28 ℃...
Embodiment 2
[0037] Embodiment 2: the automatic production method of the raw barley koji of multi-bacteria rice wine of artificial inoculation
[0038] Wort solid medium and bran medium were prepared according to the method described in the summary of the invention.
[0039] Aspergillus niger CCTCCM2015202 and Aspergillus oryzae CCTCCM2015201 were inoculated in eggplant bottle wort solid medium respectively, and cultured at 33°C for 4 days. Wash the spores separately with sterile water to make a spore suspension.
[0040] The spore suspensions of Aspergillus niger and Aspergillus oryzae were respectively inoculated into the bran medium of the Erlenmeyer flask, cultured at 33°C for 4 days, and shaken once every 24h.
[0041] The first-grade bran seeds of Aspergillus niger and Aspergillus oryzae were inoculated into the bran culture medium of the koji culture machine (ZQB-50BJ type koji culture machine) respectively according to the inoculation amount of 1%. The controlled temperature was 3...
Embodiment 3
[0044] Example 3: Multi-strain rice wine raw wheat koji applied to rice wine brewing
[0045] (1): Mix 30kg of glutinous rice and 36kg of water, keep warm at 39°C for 14h, and separate the rice and water;
[0046] (2): The soaked glutinous rice is steamed until soft and rotten without white heart, and at the same time, it can be whole without breaking.
[0047] (3): Weigh 0.3kg of commercial Angel rice wine yeast, add it to 3kg of water, and keep warm at 38°C for 30min;
[0048] (4): Put the material obtained in steps 2 and 3 into a fermenter, add 45 kg of water, and 1 kg of raw wheat koji for the above-mentioned multi-bacteria rice wine, stir evenly;
[0049] (5): Control the pre-fermentation temperature to 25°C, lower the temperature to 16°C after 7 days of fermentation, and finish the rice wine brewing after 23 days of fermentation.
[0050] The yellow rice wine of the control group adopts the above-mentioned same process, but the raw wheat koji of the multi-strain yellow...
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