Controlled culture method of Aspergillus niger for producing citric acid

A cultivation method and technology for Aspergillus niger, which are applied in the field of controlled cultivation of Aspergillus niger, can solve the problems of inapplicability to mass production, insufficient sterilization effect, easy agglomeration of culture medium, etc., and achieve easy operation, increase productivity, and save costs. Effect

Active Publication Date: 2016-05-04
JIANGSU GUOXIN UNION ENERGY CO LTD +1
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Problems solved by technology

However, in the sterilization process using such bran medium, there are problems that the medium is easy to agglomerate and the sterilization effect is not high en

Method used

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  • Controlled culture method of Aspergillus niger for producing citric acid

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Embodiment 1

[0022] 1. Preparation of large bran koji culture medium

[0023] The composition of the large bran koji medium is corncob particles, which have been dehydrated. Weigh 2000g of big bran koji and pour it into the bran koji bucket. Add deionized water according to the ratio of big bran koji: water = 1:1.8, and sterilize it for later use. .

[0024] 2 Culture of spores

[0025] (1) First-generation strain culture

[0026] Use the black mold CICC40021 strain preserved by China Industrial Microbial Strain Collection Management Center (CICC) as the cultured strain, scrape a ring, put the Aspergillus niger strain preserved by our company in 100mL sterile water, shake for 20min, and filter with absorbent cotton For spore liquid, use sterile water to dilute the spore liquid to 10 4 , and then pipette 100 μL to coat the plate, the culture temperature was 35° C., and the culture time was 36 h. A single colony with good growth was selected and inoculated on the slant medium of the test...

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Abstract

The invention discloses a controlled culture method of Aspergillus niger for producing citric acid and aims to overcome defects that the workload is heavy, the operation is inconvenient, the method is not applicable to mass production and the like during usage of a wheat bran culture medium in the prior art. Mouldy bran taking corncob grans as the culture medium component is used, and the culture mode is innovated, so that the elimination rate is decreased, the cost is reduced, and the working efficiency is improved; meanwhile, the operation is convenient, and above all, the capacity is improved.

Description

technical field [0001] The invention relates to the field of microorganism cultivation, in particular to a method for controlling cultivation of Aspergillus niger producing citric acid. Background technique [0002] Citric acid is an important and high-yield organic acid. Aspergillus niger is an important strain of citric acid production. It is a common fungus and mainly reproduces with spores. Therefore, cultivating spores is a key step in production. [0003] Citric acid fermentation generally adopts the secondary fermentation method of Aspergillus niger, that is, the mature seeds are cultivated first, and then the mature seed liquid is transferred to fermentation culture. The traditional seed culture process uses the inoculation method of Aspergillus niger spores, which requires a large number of mature Aspergillus niger spores, and the preparation process of Aspergillus niger spores is relatively complicated. A batch of mature Aspergillus niger spores needs to be screen...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P7/48C12R1/685
CPCC12N1/14C12P7/48
Inventor 胡志杰陈坚石贵阳蒋小东蒋建秋孙福新傅静高超蒋敏
Owner JIANGSU GUOXIN UNION ENERGY CO LTD
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