Method for improving yield of resveratrol through fermentation of aspergillus niger

A technology of Aspergillus niger fermentation and resveratrol, which is applied in the field of microbial fermentation and achieves the effects of remarkable technical effect, improved yield and easy popularization

Inactive Publication Date: 2015-03-04
SOOCHOW KH BIO SCI & TECH CO LTD KHB
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[0011] The following will be described in conjunction with specific embodiments.

[0012] The main conditions for accumulative fermentation of Aspergillus niger strains to transform polydatin in Polygonum cuspidatum are: temperature, inoculation amount, inoculation age, and fermentation humidity.

[0013] The inoculum size, bacterial age, and humidity were fixed at 2%, 36h, and 2:1, respectively, and the fermentation temperature was set at 4 gradients of 30, 40, 50, and 60°C to carry out stacking fermentation, and samples were taken every 6 hours to analyze the transformation of polydatin into white The case of veratrol. In the early stage of fermentation, the conversion rate of polydatin increased with the increase of fermentation temperature, and the conversion degree also increased. However, when the temperature was higher than 40℃, the content of resveratrol reached the peak at 30 hours of fermentation, and then gradually decreased.

[0014] The strains with different age...

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Abstract

The invention discloses a method for improving yield of resveratrol through fermentation of aspergillus niger. An aspergillus niger strain is cultured in a triangular flask culture medium for 48h, 2% by mass of hypha is evenly added to giant knotweed rhizomes with humidity of 2:1, and the stacking fermentation is carried out at a constant temperature of 40 DEG C for 30h. Through a solid stacking fermentation method, polydatin in giant knotweed rhizomes is converted into resveratrol, and the solid fermentation condition is optimized. In the method, a strong acid-base reaction is not generated, and glycoside does not need to be crudely extracted; meanwhile, expensive equipment does not need to be added, and the method is easy to popularize. The extraction yield of resveratrol is increased to 1.197% and is increased by 30% compared with that of the existing method, so the very obvious technical effects are achieved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to the application of Aspergillus niger, in particular to a method for increasing the yield of resveratrol by fermentation of Aspergillus niger. Background technique [0002] The active ingredients of Polygonumcuspidatum are mainly stilbenes and anthraquinones. The former is mainly resveratrol and its glycosides, and the latter is mainly emodin and its glycosides. Among them, stilbenes have remarkable and unique effects . Resveratrol has anti-tumor, anti-oxidation, anti-free radical, anti-thrombosis, anti-allergy, anti-atherosclerosis and preventive effects on coronary heart disease, ischemic heart disease, hyperlipidemia, etc. Resveratrol has been It is listed as one of the most promising anti-cardiovascular and anti-cancer drugs. According to reports, the content of resveratrol in the rhizome of Polygonum cuspidatum is 0.1%-0.2%, and the content of polydatin is about 2%. How t...

Claims

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Application Information

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IPC IPC(8): C12P7/22C12R1/685
CPCC12P7/22
Inventor 金京勋
Owner SOOCHOW KH BIO SCI & TECH CO LTD KHB
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