Method for improving resveratrol yield by using aspergillus niger to ferment

A technology for fermenting Aspergillus niger and resveratrol, which is applied in the field of microbial fermentation to achieve the effects of easy promotion, significant technical effects, and increased yield

Inactive Publication Date: 2018-02-23
黄增才
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Problems solved by technology

However, there is still room for further improvement in this method

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Embodiment Construction

[0011] The following will be described in conjunction with specific embodiments.

[0012] The main conditions for accumulative fermentation of Aspergillus niger strains to transform polydatin in Polygonum cuspidatum are: temperature, inoculation amount, inoculation age, and fermentation humidity.

[0013] The inoculum size, bacterial age, and humidity were fixed at 2%, 36h, and 2:1, respectively, and the fermentation temperature was set at 4 gradients of 30, 40, 50, and 60°C to carry out stacking fermentation, and samples were taken every 6 hours to analyze the transformation of polydatin into white The case of veratrol. In the early stage of fermentation, the conversion rate of polydatin increased with the increase of fermentation temperature, and the conversion degree also increased. However, when the temperature was higher than 40℃, the content of resveratrol reached the peak at 30 hours of fermentation, and then gradually decreased.

[0014] The strains with different age...

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Abstract

The invention discloses a method for increasing the yield of resveratrol by fermenting Aspergillus niger. The Aspergillus niger species is cultured in the culture medium of the Erlenmeyer flask for 48 hours, and the mycelium is evenly added to the moistening medium according to the mass percentage of 2%. : 1 in the Rhizome of Polygonum cuspidatum, stacked and fermented for 30h at a constant temperature of 40°C. The invention adopts a solid accumulation fermentation method to convert the polydatin in Polygonum cuspidatum into resveratrol, and optimizes the solid fermentation conditions. This method does not have a strong acid-base reaction, does not need crude glycoside extraction, and does not need to add expensive equipment, so it is easy to popularize. The resveratrol extraction yield of the present invention is increased to 1.197%, which is 30% higher than that of the existing method, and a very significant technical effect has been achieved.

Description

technical field [0001] The invention belongs to the field of microbial fermentation and relates to the application of Aspergillus niger, in particular to a method of using Aspergillus niger to ferment and transform Polygonum cuspidatum to increase the yield of resveratrol. Background technique [0002] Knotweed Polygonum cuspidatum The active ingredients of stilbenes and anthraquinones are mainly stilbenes and anthraquinones. The former is mainly resveratrol and its glycosides, and the latter is mainly emodin and its glycosides. Among them, stilbenes have remarkable and unique effects. Resveratrol has anti-tumor, anti-oxidation, anti-free radical, anti-thrombosis, anti-allergy, anti-atherosclerosis and preventive effects on coronary heart disease, ischemic heart disease, hyperlipidemia, etc. Resveratrol has been It is listed as one of the most promising anti-cardiovascular and anti-cancer drugs. According to reports, the content of resveratrol in dried Polygonum cuspidatum...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C12R1/685
CPCC12P7/22
Inventor 黄增才
Owner 黄增才
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