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A kind of Penicillium xfsf-8 strain for preventing and treating tobacco bacterial wilt and its application

A technology of tobacco wilt bacteria and Penicillium, applied in the directions of application, fungi, microorganism-based methods, etc., can solve the problems of great influence on tobacco yield and quality, high morbidity and lethality, economic loss, etc. Control effect, good effect, strong targeted effect

Active Publication Date: 2019-07-19
湖北省烟草公司恩施州公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disease has a high morbidity and mortality rate in my country's tobacco fields, and has a great impact on the yield and quality of tobacco, causing huge economic losses
At present, it is still difficult to use a single method to completely eradicate tobacco bacterial wilt, and the combination of multiple biological control and agricultural measures can reduce the harm of bacterial wilt

Method used

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  • A kind of Penicillium xfsf-8 strain for preventing and treating tobacco bacterial wilt and its application
  • A kind of Penicillium xfsf-8 strain for preventing and treating tobacco bacterial wilt and its application
  • A kind of Penicillium xfsf-8 strain for preventing and treating tobacco bacterial wilt and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Detection of the resistance of Penicillium xfsf-8 to R. solanacearum

[0034] Pick Penicillium xfsf-8 isolated and purified from the rhizosphere soil of healthy tobacco plants and inoculate it in PDA liquid medium, and culture it on a shaker at 28°C and 180rpm / min for 5 days. Sterilize by filtration through a 0.22 μm filter.

[0035] Pick a single colony of Ralstia solanacearum from the LB plate and inoculate it in LB liquid medium, culture it on a shaker at 37°C and 180 rpm / min for 24 hours, take an appropriate amount of bacterial solution and dilute it to about OD value 0.2, and spread it on the LB plate . Put the sterilized filter paper on the plate, use a pipette to take 10 μl of sterile Penicillium xfsf-8 fermentation liquid and soak it on the filter paper, and observe the inhibition zone after constant temperature cultivation at 28°C for 24 hours.

[0036] The result shows: if figure 1 , there is an obvious inhibition zone around the filter paper, and...

Embodiment 2

[0037] The optimization of embodiment 2 Penicillium xfsf-8 solid fermentation conditions

[0038] (1) Optimum carbon source and culture medium optimization

[0039] Use corn flour, wheat flour, bran, and rice bran as the carbon source, and nutrient soil and vermiculite soil as the culture substrate. Weigh 7.5g of the carbon source and the culture substrate at a ratio of 1:1 and mix them evenly before placing them in a petri dish. , sterilized at 121°C for 20 minutes for later use, and a total of 8 culture media were prepared. Three replicates were set up for each carbon source.

[0040] Take the cultured Penicillium xfsf-8 solid PDA plate, wash the spores with sterile water under the sterile operating table to make a spore suspension, measure the spore content with a hemocytometer, and adjust the concentration to 1.2×10 7 spores / ml of spore suspension.

[0041] Under sterile conditions, add 6ml of the above-mentioned spore suspension to the petri dish, mix well and place it...

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Abstract

The present invention relates to a Penicillium xfsf‑8 strain and its application in the preparation of anti-tobacco bacterial wilt fungus agent. The present invention obtains a plant of Penicillium spp. Penicillium xfsf‑8, and it has been verified by field experiments: Penicillium xfsf‑8 is effective in controlling tobacco bacterial wilt, is highly targeted, and has no pollution. It can be stored for a long time after being made into an inoculant, which is convenient for field application, and further enriches the line setting The achievements in the research field of bacterial biocontrol have guiding significance for the prevention and treatment of tobacco bacterial wilt.

Description

technical field [0001] The invention specifically relates to the optimization of Penicillium xfsf-8 solid fermentation conditions and its application in the prevention and treatment of tobacco bacterial wilt, which belongs to the field of agricultural microbiology technology and fermentation engineering. Background technique [0002] Tobacco bacterial wilt is a soil-borne bacterial disease caused by Ralstonia solanacearum. The main pathogenic feature of bacterial wilt is that the root and lateral roots of the diseased plant turn black and rot, and black streaks can be seen on the stem. The disease has a high morbidity and mortality rate in my country's tobacco fields, and has a great impact on the yield and quality of tobacco, causing huge economic losses. At present, it is still difficult to use a single method to completely eradicate tobacco bacterial wilt, and the combination of multiple biological control and agricultural measures can reduce the harm of bacterial wilt. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P1/00C12R1/80
CPCA01N63/30C12N1/145C12R2001/80
Inventor 王瑞赵秀云施河丽韩非谭军任加庆祁高富姜乾坤潘广为向必坤
Owner 湖北省烟草公司恩施州公司
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