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Culture method and application of lactobacillus acidophilus

A technology of Lactobacillus acidophilus and culture method, which is applied in the field of microbial preparation cultivation and agricultural planting fertilizer, can solve the problems of small fermentation scale and high cost, and achieve the effects of improving stability, improving inhibition and increasing diversity

Active Publication Date: 2018-08-31
ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0012] The object of the present invention is to provide a large-scale cultivation method of Lactobacillus acidophilus in order to overcome the disadvantages of small fermentation scale and high cost in the traditional culture method of Lactobacillus acidophilus

Method used

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  • Culture method and application of lactobacillus acidophilus
  • Culture method and application of lactobacillus acidophilus
  • Culture method and application of lactobacillus acidophilus

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Embodiment Construction

[0036] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0037] Basic embodiment

[0038] A kind of probiotic solid beverage, comprising by weight: 40-60 parts of inulin, 10 parts of galacto-oligosaccharide, 12 parts of fructo-oligosaccharide, 2-8 parts of Lactobacillus acidophilus, 4-20 parts of Bifidobacterium lactis, 2-10 parts of Bifidobacterium bifidum, 4-16 parts of Bifidobacterium animalis, 2-10 parts of Lactobacillus helveticus, 2-8 parts of Bifidobacterium longum, 4-20 parts of Lactobacillus casei, 2-8 parts of Lactobacillus plantarum 2-8 parts of Lactobacillus reuteri, 4-16 parts of Lactobacillus rhamnosus and 2-8 parts of Streptococcus thermophilus.

[0039] In some ...

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Abstract

The invention relates to a large-scale culture method of lactobacillus acidophilus. The method is characterized by comprising the steps as follows: 1) preparation of a strain: the strain consists of aspergillus niger spores and lactobacillus acidophilus in the weight ratio being (8-9):1; 2) preparation of a culture medium: the culture medium consists of rice bran, soybean meal, NaCl and wheat bran, and all substance components are mixed and stirred uniformly; 3) solid fermentation: the culture medium is poured in a solid fermentation tank, the solid fermentation tank is inoculated with the aspergillus niger spores, the relative humidity and the air supply are kept unchanged, and culture is performed for 48 h. The method realizes industrial solid fermentation and low-cost and large-scale culture and has the benefits of low cost and high production efficiency.

Description

technical field [0001] The invention relates to a large-scale cultivation method of Lactobacillus acidophilus, which belongs to the field of agricultural planting fertilizers, in particular to the technical field of microbial preparation cultivation. Background technique [0002] Lactobacillus acidophilus is an important member of the lactic acid bacteria family, a Gram-positive bacillus belonging to the genus Lactobacillus, widely present in the intestinal tract of animals. Lactobacillus acidophilus can secrete antibiotic substances (acidolin, acidophilin, and laetocidon), which can antagonize intestinal pathogenic bacteria and regulate intestinal Functions such as flora balance, maintaining intestinal health and improving the body's immunity are considered to be the most ideal probiotics to replace antibiotics. [0003] In addition, Lactobacillus acidophilus has strong resistance to acid, salt and bile, and can grow and reproduce in acidic and high-salt environments where...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14A23L2/39A23L2/52A23L2/42C12R1/23C12R1/685
CPCA23L2/39A23L2/42A23L2/52C12N1/14C12N1/20A23V2002/00A23V2400/147A23V2400/125A23V2400/113A23V2400/173A23V2400/175A23V2400/515A23V2400/517A23V2400/249A23V2400/531A23V2400/533A23V2400/169A23V2200/3204A23V2250/28A23V2250/5062
Inventor 张烨刘海霞耿然
Owner ZHONGKE YIKANG BEIJING BIOTECH CO LTD
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