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Aspergillus niger variant and its fermentation process in solid medium

A fermentation process, the technology of Aspergillus niger, applied in the biological field, can solve the problems of reducing the nutritional value of feed, chyme retention, pancreas enlargement, etc., and achieve the effects of improving livestock and poultry production performance, low production cost, and high enzyme activity

Inactive Publication Date: 2006-12-27
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are high levels of α-galactosides in bean seeds and cake feed, which not only reduce the nutritional value of the feed, but also easily cause chyme retention in the hindgut of animals, resulting in intestinal flatulence , digestive disorders, causing pancreas enlargement, diarrhea and other diseases

Method used

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  • Aspergillus niger variant and its fermentation process in solid medium

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Effect test

Embodiment 1

[0034] 1. Bacteria culture:

[0035] 1, slant strain cultivation: Aspergillus niger variant RM45# of the present invention is inoculated on the slant of Potato glucose agar medium (PDA), cultivated at a constant temperature of 72-96 hours at 28-30° C., after growing plump yellow-brown spores , make spore suspension with sterile water (concentration is 10 7 -10 8 pcs / ml), for use.

[0036] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The culture medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the suspension of spores of slant bacteria, fully stirred evenly, and 30 grams of water-containing and inoculated culture medium is filled into each 500 ml triangular flask.

[0037] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of the medium g...

Embodiment 2

[0053] 1. Bacteria culture:

[0054] 1, slant strain cultivation: Aspergillus niger variant RM45# of the present invention is inoculated on the slant of Potato Ru glucose agar medium (PDA), cultivated at a constant temperature for 96 hours at 28-30° C., after growing plump yellow-brown spores, use Spore suspension was prepared in sterile water (concentration of 10 7 -10 8 pcs / ml), for use.

[0055] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The culture medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the suspension of spores of slant bacteria, fully stirred evenly, and 30 grams of water-containing and inoculated culture medium is filled into each 500 ml triangular flask.

[0056] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of th...

Embodiment 3

[0072] 1. Bacteria culture:

[0073] 1, slant strain cultivation: Aspergillus niger variant RM45# of the present invention is inoculated on the slant of Potato glucose agar medium (PDA), cultivated at a constant temperature of 72-96 hours at 28-30° C., after growing plump yellow-brown spores , make spore suspension with sterile water (concentration is 10 7 -10 8 pcs / ml), for use.

[0074] 2. Solid seed culture: 1.5kg bran is added 1% (NH 4 ) 2 SO 4 The solution, after being thoroughly mixed, is made into a solid seed medium. The culture medium is sterilized at 121° C. for 30 minutes, and after cooling, 1-5% of the culture medium is added to the suspension of spores of slant bacteria, fully stirred evenly, and 30 grams of water-containing and inoculated culture medium is filled into each 500 ml triangular flask.

[0075] 3. The inoculated solid seed culture medium is incubated at a constant temperature of 28-30°C for 72 hours. After the surface and inside of the medium g...

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Abstract

The invention relates the aspergillus Niger variant, and spore is ochre. The bacterial comprises the following biology characters: the bacterial is on the culture medium to grow for 7 days at 25Deg.C, the diameter is 45-70mm; the texture is villiform and granular; conidiospore is light brown; the reverse surface of bacterial colony is yellow. Using the aspergillus Niger variant, and adopting solid invoice method, the alpha-galactosidase is made. The enzymatic activity is 286IU / g, and the maximum is 345IU / g.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a variety of Aspergillus niger and its fermentation process on a solid substrate. Background technique [0002] α-galactosidase is an enzyme that catalyzes the decomposition of α-galactosides into monosaccharides such as oligosaccharides, sucrose, and fructose, and is widely found in microorganisms, plants, and animals. α-Galactosidase has very important application value in all aspects of food and pharmaceutical industries. In the beet sugar industry, the raffinose in molasses is decomposed by α-galactosidase, which can not only increase the yield, but also improve the efficiency. In addition, it is also widely used in medicine and modification of polysaccharide gums. Studies in recent years have shown that this enzyme, as a specific exogenous feed enzyme, also has a broad application prospect in the feed industry. There are high levels of α-galactosides in bean seeds and cake fe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/685
Inventor 许尧兴许少春李艳丽姚晓红李孝辉
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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