The invention discloses a detecting kit capable of fast, simply and simultaneously detecting 6 aquatic product pathogenic bacteria such as the pseudomonas aeruginosa and the like. The kit comprises an oxidase test paper, a hydrogen sulfide culture medium, a halophilic identifying reagent, an ortho-nitrophenol beta-D-galactoside culture medium, a pectinose culture medium, a lactobiose culture medium, saccharose culture medium, a glucose culture medium, a mannose culture medium, a cellobiose culture medium, a dextrose oxidation and fermentation identifying reagent, an arginine bi-hydrolytic enzyme identifying reagent, an ornithine identifying reagent, a lysine identifying reagent, an amino acid decarboxylation identifying reagent and an indol identifying reagent. The kit has simple reagent composition and preparation method and use method, has low cost, does not need expensive apparatuses and operators with mature technology, compared with a bacterium fast automatic identifying system and a detecting technology based on the nucleic acid. The detecting kit is suitable for initially detecting the normal pathogenic bacteria of aquatic animals, is particularly suitable for the use of aquatic product popularization staff at the production line, and immediately excludes the difficulty and the anxiety for fishing peasants.