The invention discloses a preparation method of an acellular biological dermal material. The method comprises the following steps: 1,
processing pigskins with an EDTA-containing
trypsin solution, flushing the processed pigskins, immersing and vibrating the flushed pigskins in a scale remover solution, and flushing the immersed pigskins; 2,
processing the above obtained processed material with a
neutral protease solution, flushing the processed material, immersing and vibrating the flushed material in the scale remover solution, and flushing the immersed material; and 3, carrying out step 1 and step 2 1-3 times,
processing the processed material with an alpha-1,3-galactosidase solution, and flushing the material to obtain the acellular biological dermal material. The acellular biological dermal material which is prepared after a series of physical and chemical treatments in the invention has the characteristics of safety, no
toxicity,
decellularization, no alpha-1,3-galactosidase and no
immunogenicity, reserves the structure of natural collagen, has very good
toughness and strength, can be used as an implantable
biological tissue material in the fields of
surgery, shaping,
hernia restoration,
abdominal cavity restoration and
pelvic cavity restoration, and also can be used for restoring
skin wounds.