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67 results about "Galactosides" patented technology

Glycosides formed by the reaction of the hydroxyl group on the anomeric carbon atom of galactose with an alcohol to form an acetal. They include both alpha- and beta-galactosides.

Acellular biological dermal material, and preparation method and application thereof

The invention discloses a preparation method of an acellular biological dermal material. The method comprises the following steps: 1, processing pigskins with an EDTA-containing trypsin solution, flushing the processed pigskins, immersing and vibrating the flushed pigskins in a scale remover solution, and flushing the immersed pigskins; 2, processing the above obtained processed material with a neutral protease solution, flushing the processed material, immersing and vibrating the flushed material in the scale remover solution, and flushing the immersed material; and 3, carrying out step 1 and step 2 1-3 times, processing the processed material with an alpha-1,3-galactosidase solution, and flushing the material to obtain the acellular biological dermal material. The acellular biological dermal material which is prepared after a series of physical and chemical treatments in the invention has the characteristics of safety, no toxicity, decellularization, no alpha-1,3-galactosidase and no immunogenicity, reserves the structure of natural collagen, has very good toughness and strength, can be used as an implantable biological tissue material in the fields of surgery, shaping, hernia restoration, abdominal cavity restoration and pelvic cavity restoration, and also can be used for restoring skin wounds.
Owner:浙江华臻医疗器械有限公司

Caffeic acid glucoside derivative and preparation method thereof

The invention relates to a caffeic acid glucoside derivative and a preparation method thereof. The caffeic acid galactoside mixture comprises the active ingredients of caffeic acid 3'-O-beta-D-pyran galactoside and caffeic acid 4'-O-beta-D-pyran galactoside, and the mixture has the molecular formula of C15H18O9. The invention also relates to a method for preparing the caffeic acid galactoside mixture. The caffeic acid galactoside is synthesized by employing the mutational beta-galactosidase, and compared with the raw material caffeic acid, the product contains galactosyl, the water solubility and stability are improved, and the application range is widened.
Owner:SHANDONG UNIV

Method for producing lactulose through whole-cell catalysis

The invention discloses a method for producing lactulose through whole-cell catalysis, and belongs to the field of the food biotechnology. According to the method, recombinant escherichia coli E. coli BL 21 (DE3) for producing cellobiose epimerase is taken as production bacterial strains, lactose is used for replacing isopropyl-beta-D-sulfo-galactoside (IPTG) and taken as an inductive agent for fermental cultivation, and thalluses obtained through centrifugation are subjected to ethyl alcohol permeabilization and vacuum freeze drying to serve as a cell biocatalyst for directly converting the lactose to produce the lactulose. The maximum percent conversion of the lactulose can reach 65.1%, the concentration of the lactulose reaches 390.6 g / L, the production rate of the lactulose reaches 195.3 g / (L*h), and the production quantity of by-product epidepride lactulose is smaller than 2%(w / w). According to the method, microbial cells are directly used for converting the lactose to produce the lactulose, and the method is simple and easy to implement, avoids the loss of enzyme activity in the separation and purification process, greatly reduces the cost of producing the lactulose through an enzymic method and is beneficial for achieving industrialization of lactulose production through the enzymic method early.
Owner:JIANGNAN UNIV

Method for preparing probiotic additive for yellow wine lees feed

The invention provides a method for preparing a probiotic additive for a yellow wine lees feed. The multi-functional probiotic additive for the yellow wine lees feed is prepared by compounding multiple probiotic strains, namely Clostridium butyricum, Lactobacillus fermentum ML7, Lactobacillus fermentum La-YI, and Bifidobacterium animalis subsp. lactis Qq08. The additive can regulate intestinal floras of animals and recover intestinal flora disturbance caused by antibiotics, has the functions of reducing emergency and heat-sensitive reactions, effectively degrades nutritional antagonists with heavy components such as alpha-galactosides and the like in cake feeds, remarkably improves feed conversion ratio, has the effect of degrading cholesterol, reduces the absorption of the cholesterol inintestines, and reduces the content of the cholesterol in animal products; and the additive can improve the palatability of the yellow wine lees feed, and increase the appetite of livestock so as to increase the feed intake.
Owner:绍兴市古煌酿酒有限公司

Application of 2'-O-beta-L-galactopyranosyl orientin and composition in preparing medicaments for resisting myocardial ischemia

The invention discloses novel application of composition with the content of flavone C-glycoside 2'-O-beta-L-galactopyranosyl orientin and 2'-O-beta-L-galactopyranosyl orientin between 30 and 90 percent in preparing medicaments for resisting angiocardiopathy such as myocardial ischemia and the like, and also describes a single component of the 2'-O-beta-L-galactopyranosyl orientin, a method for preparing the composition, and a medicinal preparation.
Owner:JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT

Coli group and colibacillus testing medium

The invention discloses a coli group and colibacillus testing medium which is prepared by dissolving 20 grams of peptone, 5 grams of lactose, 2 grams of bile salt, 1.5 grams of monopotassium phosphate, 4 grams of monopotassium phosphate, 5 grams of sodium chloride, 0.1 gram of 5-bromo-4-chloro-3-indole galactoside, 0.1gram of 4- methylumbelliferone glucuronide, 1 gram of aspartic acid, 0.5 gram of casamino acid, 1 gram of yeast extracts and 20 grams of agar in 1000ml of deionized water, and the coli group and colibacillus testing medium is not only used as a plate medium, can also be used as an analoids form, and is low in cost and convenient to popularize and use.
Owner:STONE LAKE PHARMA TECH

Type two peptidoglycan recognition protein and preparation method and application thereof

The invention relates to the field of molecule microbiology, in particular to a type two peptidoglycan recognition protein (PGRP) of sciaenops ocellatus and a preparation method and application of the type two peptidoglycan recognition protein of the sciaenops ocellatus. PGRP is shown as an amino acid sequence in a sequence table sequence (SEQ) ID number (No.) 1. The preparation method comprises the steps that plasmid p PGRP2 is converted to escherichia coli BL 21 (DE3) to obtain BL21 or the p PGRP 2, the BL21 or the p PGRP 2 after the inducement of isopropyl-beta-D-sulfo-galactoside recombines protein through the purification of affinity chromatography, namely, the protein has bacteriostatic activity. The type two PGRP has the function of specificity fungicide which aims at gram-positive bacterium, so that the growth or the infection of the gram-positive bacterium can be retrained.
Owner:INST OF OCEANOLOGY - CHINESE ACAD OF SCI

Method for synthesizing soybean oligosaccharides through biological catalysis

The invention discloses a method for synthesizing soybean oligosaccharides through biological catalysis. The method has the beneficial effects that that through establishing an in-vitro multi-enzyme cascade reaction system composed of sucrose synthetase, uridine diphosphate glucose 4-differential isomerase, inositol galactoside synthase, raffinose synthase and threonose synthase, sucrose is takenas a substrate to synthesize a single component or a mixed component in inositol galactoside, raffinose and threonose; the method realizes the recycling conversion of uridine diphosphate and inositol,and a crude enzyme solution added in the system contains a small amount of uridine diphosphate, so that the expensive uridine diphosphate can be avoided; the inositol galactoside yield reaches 50 g / L, the conversion rate is 70 percent, the raffinose yield reaches 20 g / L, the threonose yield reaches 10 g / L, and the obtained inositol galactoside, raffinose and threonose can be used in the fields offood, medicines and the like; the method disclosed by the invention is of great significance in the utilization of high attachment values of the sucrose and a biomass rich in the sucrose.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI

Galactoside compound and preparation method thereof

ActiveCN102241708ASweetenerFunctionalSugar derivativesFermentationBeta-d-galactopyranoseBeta-Galactosidases
The invention relates to a galactoside compound and a preparation method thereof, and particularly relates to a method for synthesizing a galactoside compound beta-6-galactosyl-sucralose by utilizing beta-galactosidase, belonging to the technical field of sugar engineering. The galactoside compound has a chemical name of 1,6-dichloro-1,6-dideoxy-beta-D-fructofuranosyl-4-chloro-4-deoxy-alpha-D-galactopyranosyl-beta-D-galactopyranose and a molecular formula of C18H29O13C13.
Owner:SHANDONG UNIV

Stem cell cryopreservation liquid as well as preparation method and application thereof

The invention discloses a stem cell cryopreservation solution as well as a preparation method and application thereof. The cryopreservation liquid is prepared from the following components in percentage by volume: 20 to 40 percent of a basic culture medium, 10 to 20 percent of glycerol, 30 to 60 percent of human serum albumin, 2 to 6 percent of trehalose, 0.5 to 2 percent of glycine or taurine and 6 to 10.5 percent of inositol galactoside or glycoside. According to the cryopreservation liquid, the serum-free cryopreservation liquid is adopted for cryopreservation of the stem cells, the cells are free of damage, the possibility of animal pathogen pollution can be reduced, the inositol galactoside or the glycoside serves as the stabilizer, the cell phenotype can be effectively protected, the activity of the stem cells under the cryopreservation condition can be remarkably improved, and therefore, the physiological function and the biological function of the recovered cells are maintained.
Owner:东莞再立健生物科技有限公司

Reduction of non-starch polysaccharides and alpha-galactosides in soy flour by means of solid-state fermentation using cellulolytic bacteria isolated from different environments

InactiveUS20150030637A1Low costDecreases subsequent drying costBacteriaBacteria material medical ingredientsCelluloseBacterial strain
The invention relates to a method for fermenting soy flour in the solid state in order to reduce non-starch polysaccharides and alpha-galactosides, said method comprising the following steps: a) preparation of the fermentation substrate; b) inoculation of the substrate with selected celluloytic bacterial strains; c) incubation; and, optionally, d) drying of the product, which generates a product with: an increase in protein of between 12 and 15% compared to non-fermented soy flour, degradation of the alpha-galactosides of more than 90% compared to non-fermented soy flour, a reduction in non-starch polysaccharides (NSPs) of between 15 and 25%, an amino acid profile similar to that of non-fermented soy flour, and immune-stimulating effects.
Owner:UNIVERSITY OF CHILE

Chickweed flavonoside extract, its preparation method and medicine made therefrom application

The invention relates to extractive of chickweed flavone glycoside, containing apigenin-6-C-Beta-D-galactose-8-C-Alpha-L-arabinoside and apigenin-6-C-Alpha-L-arabinoside-8-C-Beta-D-galactoside. The invention also relates to the extraction method of the chickweed flavone glycoside extraction, the drug compound containing the extractive and the purpose for preparing lipid lowering drug.
Owner:INST OF PHARMACOLOGY & TOXICOLOGY ACAD OF MILITARY MEDICAL SCI P L A

Method for separating and preparing petunidin 3-galactoside chloride

The invention provides a method for separating and preparing petunidin 3-galactoside chloride. The method comprises alcohol extraction and concentration, macroporous resin adsorption, preparative liquid chromatography purification and high-speed counter-current chromatography separation, and a high-purity petunidin 3-galactoside chloride monomer is isolated and prepared from blueberry raw materials with complicated composition of anthocyanin. The method combines preparative liquid chromatography and high-speed counter-current chromatography technologies for the first time, the high-purity petunidin 3-galactoside chloride monomer is isolated and obtained from blueberries by optimizing process parameters, and the purity is up to 99%.
Owner:ZHEJIANG UNIV

Composite using orientin-2'-O-beta-L-galactoside as main component and application thereof

The invention discloses a composite using orientin-2'-O-beta-L-galactoside as a main component, which is prepared by performing extraction on dried flowers of trollius chinensis bunge, trollius ledebouri reichb and trollius macropetalus fr. schmidt. Pharmacological experiments show that the composite has the effects of preventing and treating prostatitis, prostatic hyperplasia and the like. The composite has new application in the preparation of medicaments for preventing and treating prostatic diseases. The medicinal preparation of the composite may be any one of tablets, capsules, pills, grains, suspension, dropping pills, paste, suppository rectal administration preparation and the like.
Owner:JILIN MODERN TRADITIONAL CHINESE MEDICINE ENG RES CENT

Preparation method of 4-methyl umbrella-shaped keto-beta-D-pyran galactoside

The invention discloses a preparation method of 4-methyl umbrella-shaped keto-beta-D-pyran galactoside. The method comprises the following steps: step 1, preparing 4-methyl umbrella-shaped ketone; step 2, preparing tetra acetyl-Alpha-D-pyran galactoside; step 3, preparing 4-methyl umbrella-shaped keto-2,3,4,6-tetra acetyl-beta-D-pyran galactoside; and step 4, preparing the 4-methyl umbrella-shaped keto-beta-D-pyran galactoside. The method is moderate in reaction condition, namely, the reactions are carried out at a room temperature; heavy metallic salts are not required, so that the environmental friendliness is realized; the yield is high, namely, the one-step yield of glycosylations can reach 73.1% and the total yield of the glycosylations can rach 32.9%. The microbiology experiment proves that the effects of the 4-methyl umbrella-shaped keto-beta-D-pyran galactoside are equal to the effects of an imported substrate, so that the 4-methyl umbrella-shaped keto-beta-D-pyran galactoside can be used for replacing an imported product.
Owner:GUANGDONG INST OF MICROORGANISM +1

Phloroglucinol glucoside derivatives and preparation method thereof

The invention relates to phloroglucinol glucoside derivatives and a preparation method thereof. The preparation method is characterized in that galactosylated modification is carried out on phloroglucinol by using phloroglucinol as a raw material, a low-cost lactose substrate as a galactosyl donor and glycosidase modified by molecules originated from microorganisms as a tool enzyme so as to synthesize multiple phloroglucinol galactoside derivatives. Compared with the raw material phloroglucinol, the synthesized phloroglucinol galactoside derivatives have the advantages that the synthesized phloroglucinol galactoside derivatives contain galactosyl, the water solubility and the stability are improved, the application range is widened, and the synthesized phloroglucinol galactoside derivatives can serve as new chemical synthesis intermediates to be used for deriving and synthesizing new high value-added compounds.
Owner:SHANDONG UNIV

Novel hecogenin compound and extract and preparation method and application thereof

The invention relates to a novel hecogenin compound with a structural formula (I): (novel hecogenin,3-O-beta-D-glucose-(1-2)-[beta-D-glucose-(1-3)]-beta-D-glucose-(1-4)-beta-D-galactoside); after the reflowing extraction of C.tuberosum harb ethanol, obtained crude extract is implemented with column chromatography by macroporous resin, and is gradually eluted by water and ethanol; the obtained 70% ethanol eluate is implemented with ODS column chromatography, and is eluted by water and 100% acetonitrile; the 70% ethanol eluate has the purpose of inducing platelet aggregation activity aspect.
Owner:TIANJIN UNIV OF TRADITIONAL CHINESE MEDICINE

Preparation method of o-nitrobenzene galactoside

The invention provides a preparation method of o-nitrobenzene galactoside, which comprises the following steps: performing heating reaction on tetra-acetylated bromogalactose, o-nitrophenol, sodium hydroxide and tetrabutyl ammonium bromide in water and dichloromethane to obtain tetra-acetylated o-nitrobenzene galactoside; and performing deacetylation on the tetra-acetylated o-nitrobenzene galactoside to obtain the o-nitrobenzene galactoside. According to the invention, the tetra-acetylated bromogalactose and the o-nitrophenol react in a system of the tetrabutyl ammonium bromide and the sodium hydroxide, and the presence of the tetrabutyl ammonium bromide and the sodium hydroxide ensures that the o-nitrophenol and the tetra-acetylated bromogalactose react more thoroughly, thereby shortening the reaction time and improving the reaction yield.
Owner:SHENGQUAN HEALTANG

Compositions and methods for removal of detergents from aqueous solutions

Compositions and methods are described in which a primary detergent or surfactant in an aqueous solution is removed by the addition of a secondary detergent or surfactant in concentrations that exceed the critical micellar concentration (CMC) of the secondary detergent or surfactant. These compositions and methods are particularly applicable to protein-containing solutions. Typical primary detergents / surfactants include polysorbate 20, polysorbate 80, and Triton X-100. Suitable secondary detergents or surfactants can be ionic, nonionic, or zwitterionic. Typical secondary detergents / surfactants include, but are not limited to, galactoside detergents (e.g. octyl-β-galactoside), glucamide detergents (e.g. MEGA 8, MEGA 9, MEGA 10), cholamide detergents (e.g. CHAPS, CHAPSO, BIGCHAPS), and sulfobetaine detergents (such as sulfobetaine 3-10).
Owner:BIOMADISON

Application of natural eutectic solvent in selective extraction of anthocyanin and preservation of anthocyanin

The invention discloses an application of a natural eutectic solvent in selective extraction of anthocyanin, which comprises the following steps: an aqueous solution of the natural eutectic solvent isuniformly mixed with blueberry pomace powder, and an anthocyanin crude extract is extracted under ultrasonic and water bath heating conditions; the natural eutectic solvent takes choline chloride asa hydrogen bond acceptor and oxalic acid as a hydrogen bond donor; wherein the molar ratio of choline chloride to oxalic acid is 1: 0.5-2; wherein in the aqueous solution of the natural eutectic solvent, the mass ratio of water to the natural eutectic solvent is 10-50%; the anthocyanin with delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside as main components canbe obtained from the anthocyanin crude extract. The invention also discloses an application of the natural eutectic solvent in anthocyanin preservation. The eutectic solvent provided by the inventionhas strong selectivity on delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside, and can effectively improve the stability of delphinidin-3-galactoside, delphinidin-3-arabinoside and mallow pigment-3-arabinoside.
Owner:ZHEJIANG UNIV

Detection kit for alpha-galactosidase activity

The invention discloses a detection kit for alpha-galactosidase activity, and belongs to the field of biochemical detection. The detection kit comprises a reaction solution, a precipitant, a stop solution, a linear standard substance and a reference substance, wherein the linear standard substance is a 4-methylumbelliferone solution, and the reaction solution is a 4-methylumbelliferone acyl-alpha-D-galactoside solution. The kit can rapidly and accurately carry out in-vitro detection on the activity of alpha-galactosidase in a human blood sample, and can achieve the purpose of rapidly, conveniently and accurately assisting in diagnosing Fabry disease patients in batches by detecting a collected filter paper dry blood slice sample or a venous blood sample; and the kit is high in detection sensitivity, good in selectivity, strong in anti-interference capability, good in reproducibility, simple and convenient to operate, good in product stability and easy to use clinically.
Owner:诺道中科(北京)生物科技有限公司

Preparation method of p-methylphenyl-beta-D-thioacetylgalactoside

The invention relates to the field of carbohydrates and particularly relates to a preparation method of p-methylphenyl-beta-D-thioacetylgalactoside. The preparation method comprises the steps of adding acetic anhydride and a catalyst at room temperature; adding D-galactose; after the reaction is ended, dropwise adding the reaction liquid into a thiocresol and methylene dichloride solution; after the reaction is ended, carrying out after-treatment to obtain p-methylphenyl-beta-D-thioacetylgalactoside. The method is used for synthesizing p-methylphenyl-beta-D-thioacetylgalactoside through one-step reaction, and is simple in operation, easy to obtain raw materials and capable of saving operation cost and materials.
Owner:SHENGQUAN HEALTANG

Detecting kit capable of simultaneously detecting 6 aquatic product pathogenic bacteria such as pseudomonas aeruginosa, etc

The invention discloses a detecting kit capable of fast, simply and simultaneously detecting 6 aquatic product pathogenic bacteria such as the pseudomonas aeruginosa and the like. The kit comprises an oxidase test paper, a hydrogen sulfide culture medium, a halophilic identifying reagent, an ortho-nitrophenol beta-D-galactoside culture medium, a pectinose culture medium, a lactobiose culture medium, saccharose culture medium, a glucose culture medium, a mannose culture medium, a cellobiose culture medium, a dextrose oxidation and fermentation identifying reagent, an arginine bi-hydrolytic enzyme identifying reagent, an ornithine identifying reagent, a lysine identifying reagent, an amino acid decarboxylation identifying reagent and an indol identifying reagent. The kit has simple reagent composition and preparation method and use method, has low cost, does not need expensive apparatuses and operators with mature technology, compared with a bacterium fast automatic identifying system and a detecting technology based on the nucleic acid. The detecting kit is suitable for initially detecting the normal pathogenic bacteria of aquatic animals, is particularly suitable for the use of aquatic product popularization staff at the production line, and immediately excludes the difficulty and the anxiety for fishing peasants.
Owner:PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI

Use of anthocyanin 3-[2-(xyloside)galactoside] as an active ingredient in the preparation of blood lipid-lowering drugs

The invention discloses application of delphinin 3-[2-(xyloside)galactoside] as active ingredient to preparation of lipid-lowering drugs, and provides application of delphinin 3-[2-(xyloside)galactoside] and cyaniding-3-O-glucoside as active ingredients to preparation of lipid-lowering drugs. According to the application, delphinin 3-[2-(xyloside)galactoside] is sinly adopted or delphinin 3-[2-(xyloside)galactoside] and cyaniding-3-O-glucoside are reasonably matched to obtain two active ingredients for a synergistic effect, and animal experiments prove that application is capable of significantly reducing the blood lipid levels in mice, thereby providing a new pharmaceutical composition for lipid lowering for people.
Owner:JIANGSU VOCATIONAL COLLEGE OF MEDICINE

11-deoxyglycyrrhetinic acid stearyl alcohol ester, derivative, cantharidin lipidosome, preparation method and application

The invention discloses 11-deoxyglycyrrhetinic acid stearyl alcohol ester, a derivative, cantharidin lipidosome, a preparation method and the application thereof. The 11-sodium deoxyglycyrrhetinic acid stearyl alcohol ester is prepared by directly putting glycyrrhetinic acid stearyl alcohol ester into amalgam zinc for deoxidation treatment, that is, an esterification reaction is firstly carried out, deoxidation treatment is carried out later, and the 11-deoxyglycyrrhetinic acid stearyl alcohol ester and succinic anhydride are further subjected to an acylation reaction to obtain 11-deoxycarbenoxolone stearyl alcohol ester which is reacted with acetyl bromide galactoside and the like to obtain the 11-deoxyglycyrrhetinic acid stearyl alcohol ester-3-O-galactoside. The cantharidin lipidosome modified by the 11-deoxycarbenoxolone stearyl alcohol ester and the 11-deoxyglycyrrhetinic acid stearyl alcohol ester-3-O-galactoside has good liver targeting and is capable of alleviating adverse reactions of livers, inhibiting tumor cell proliferation, inducing apoptosis of tumor cells and improving medicine treatment effects, and the lipidosome is high in encapsulation efficiency.
Owner:HUNAN UNIV OF CHINESE MEDICINE
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