Aspergillus niger strain and application thereof in preparing fructo-oligosaccharide by anaerobic fermentation

A technology of anaerobic fermentation and fructooligosaccharides, which is applied in the direction of fermentation, fungi, and microbial-based methods, can solve problems such as poor color, large equipment investment, and long process routes, so as to reduce equipment investment, improve product quality, The effect of reducing energy consumption

Active Publication Date: 2010-03-03
BAOLINGBAO BIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the domestic and foreign industrialized production methods are mainly to screen out fructo-oligosaccharide-producing strains first, then crush the bacteria to prepare immobilized enzyme preparations, and use aerobic fermentation technology to produce fructo-oligosaccharides. The process route is long and the investment in equipment is large.
In addition, the aerob

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1 Get 4000 grams of sucrose and add in 15 liters of anaerobic fermentation tanks, add 6000 grams of water, after heating and dissolving, adjust the pH value of the feed liquid to 5, then insert the fructo-oligosaccharide production strains of expanded cultivation, and the strain insertion amount is 10 % (V / V), the temperature was controlled at 50°C, and the reaction time was 15 hours. After the fermentation conversion is completed, the bacteria are recovered by microfiltration membrane filtration, and the bacteria are recycled for the next batch of production. Then the feed liquid is heated to 100°C for sterilization for 10 minutes, and an appropriate amount of biological flocculant is added. The flocculation temperature is 60-90°C, and the flocculation is completed. 0.05-0.1% activated carbon is added to the feed liquid for decolorization treatment, and the treatment lasts for 30 minutes. After refining, concentration, packaging and other treatments, a finished...

Embodiment 2

[0036] Embodiment 2 Get 6000 grams of sucrose and add in 15 liters of anaerobic fermentation tanks, add 5000 grams of water, after heating and dissolving, adjust the pH value of the feed liquid to 5.5, then add the fructooligosaccharide production strains of expanded cultivation, the strain insertion amount is 20% , the temperature was controlled at 30° C., and the reaction time was 20 hours. After the fermentation conversion is completed, the bacteria are recovered by microfiltration membrane filtration, and the bacteria are recycled for the next batch of production. Then the feed liquid is heated to 100°C for sterilization for 10 minutes, and an appropriate amount of biological flocculant is added. The flocculation temperature is 60-90°C, and the flocculation is completed. 0.05-0.1% activated carbon is added to the feed liquid for decolorization treatment, and the treatment lasts for 30 minutes. After refining, concentration, packaging and other treatments, a finished liquid ...

Embodiment 3

[0037] Embodiment 3 Get 5000 grams of sucrose and add in 15 liters of anaerobic fermentation tanks, add 5000 grams of water, after heating and dissolving, adjust the pH value of the feed liquid to 6, then add the fructooligosaccharide production strains of expanded cultivation, the strains insertion amount is 15% , the temperature was controlled at 50° C., and the reaction time was 25 hours. After the fermentation conversion is completed, the bacteria are recovered by microfiltration membrane filtration, and the bacteria are recycled for the next batch of production. Then the feed liquid is heated to 100°C for sterilization for 10 minutes, and an appropriate amount of biological flocculant is added. The flocculation temperature is 60-90°C, and the flocculation is completed. 0.05-0.1% activated carbon is added to the feed solution for decolorization treatment, and the treatment is performed for 30 minutes. After refining, concentration, packaging and other treatments, a finished...

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Abstract

The invention relates to a novel method for preparing fructo-oligosaccharide, which adopts a thallus anaerobic fermentation technology to produce the fructo-oligosaccharide with the component contentover 50 percent. The method has simple process, does not need a sterile air preparation system equipped for producing the fructo-oligosaccharide by anaerobic fermentation and relevant electric power appliance, and has less equipment investment and low energy resource consumption, thereby greatly saving energy sources. The novel method is applicable to producing the fructo-oligosaccharide of Aspergillus niger BLB-11, which comprises the following steps: carrying out primary or secondary cultivation to obtain a strain for enlarging the culture; transferring the strain to an anaerobic fermentation tank; under the anaerobic condition, adjusting sucrose concentration to 10-60 percent, adding 1-40 percent of bacteroid cells, setting the pH value to be 4-7, carry out the anaerobic fermentation for 10-36 hours at the reaction temperature of 25-60 DEG C to obtain a reaction solution with the content of the fructo-oligosaccharide over 50 percent, micro-filtering, decolorizing, refining and condensation the reaction solution to obtain a fructo-oligosaccharide liquid product, drying the fructo-oligosaccharide liquid product to to obtain a solid fructo-oligosaccharide product after concentration.

Description

technical field [0001] The invention belongs to the field of fructo-oligosaccharide preparation, and relates to a method for producing fructo-oligosaccharide by anaerobic fermentation. Background technique [0002] Fructose-oligosaccharide refers to a functional oligosaccharide formed by linking 2 to 8 fructosyl groups as chain links, a glucose group as the end group of the chain, and a fructosyl→fructose linkage as the main skeleton. Fructose-oligosaccharide is a prebiotic that can activate the proliferation of human probiotics and promote the balance of human microecology. It is recognized as having health effects such as regulating gastrointestinal function, reducing blood lipids, regulating body balance, and improving immunity. A typical water-soluble dietary fiber, which has been widely used as functional ingredients or taken directly. [0003] At present, the domestic and foreign industrialized production methods are mainly to screen out fructo-oligosaccharide-produci...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P19/00C12R1/685
Inventor 栾庆民袁卫涛熊晓兰张友亮陈杰喜
Owner BAOLINGBAO BIOLOGY
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