Aspergillus niger strain and application thereof in preparing fructo-oligosaccharide by anaerobic fermentation

Abstract

The invention relates to a novel method for preparing fructo-oligosaccharide, which adopts a thallus anaerobic fermentation technology to produce the fructo-oligosaccharide with the component contentover 50 percent. The method has simple process, does not need a sterile air preparation system equipped for producing the fructo-oligosaccharide by anaerobic fermentation and relevant electric power appliance, and has less equipment investment and low energy resource consumption, thereby greatly saving energy sources. The novel method is applicable to producing the fructo-oligosaccharide of Aspergillus niger BLB-11, which comprises the following steps: carrying out primary or secondary cultivation to obtain a strain for enlarging the culture; transferring the strain to an anaerobic fermentation tank; under the anaerobic condition, adjusting sucrose concentration to 10-60 percent, adding 1-40 percent of bacteroid cells, setting the pH value to be 4-7, carry out the anaerobic fermentation for 10-36 hours at the reaction temperature of 25-60 DEG C to obtain a reaction solution with the content of the fructo-oligosaccharide over 50 percent, micro-filtering, decolorizing, refining and condensation the reaction solution to obtain a fructo-oligosaccharide liquid product, drying the fructo-oligosaccharide liquid product to to obtain a solid fructo-oligosaccharide product after concentration.

Description

technical field [0001] The invention belongs to the field of fructo-oligosaccharide preparation, and relates to a method for producing fructo-oligosaccharide by anaerobic fermentation. Background technique [0002] Fructose-oligosaccharide refers to a functional oligosaccharide formed by linking 2 to 8 fructosyl groups as chain links, a glucose group as the end group of the chain, and a fructosyl→fructose linkage as the main skeleton. Fructose-oligosaccharide is a prebiotic that can activate the proliferation of human probiotics and promote the balance of human microecology. It is recognized as having health effects such as regulating gastrointestinal function, reducing blood lipids, regulating body balance, and improving immunity. A typical water-soluble dietary fiber, which has been widely used as functional ingredients or taken directly. [0003] At present, the domestic and foreign industrialized production methods are mainly to screen out fructo-oligosaccharide-produci...

AI Technical Summary

Problems solved by technology

[0003] At present, the domestic and foreign industrialized production methods are mainly to screen out fructo-oligosaccharide-producing strains first, then crush the bacteria to prepare immobilized enzyme preparations, and use aerobic fermentation technology to produce fructo-oligosaccharides. The process route is long and the investment in equipment is large.

In addition, the aerobic fermentation technology has a large amount of ventilation, and the energy consumption of the sterile air preparation system is high

Usually prepared fructooligosaccharide products are cloudy in appearance, yellow in color, poor in color, and the light transmittance of the product is below 85%. The application range of the product is limited, and the bacteria can only be used once.