Degelatinized enzyme made from Aspergillus niger and its use in degumming of fiberflax

A technology of degumming enzyme and Aspergillus niger, which is applied in the field of degumming enzyme to achieve the effects of complete enzyme system, improved production rate and reduced hairiness

Inactive Publication Date: 2007-11-21
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, the work on flax degumming has mainly focused on the breeding of degumming bacteria and the research on the inoculation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0024] Aspergillus niger (Aspergillus niger) HYA4 is used as the strain, and the preservation number is CGMCCNo.1897. In order to enable the strain to maintain stable high-yield characteristics, the slant preservation medium uses potato dextrose agar medium; the raw material and preparation method of the slant medium are: potato Peel 200g, cut into pieces and boil for 30min, then filter with gauze, add 20g of glucose and 20g of agar, add water to 1000mL after dissolving, the pH is natural; The bacteria were inoculated, the culture temperature was 36°C, the culture time was 5 days, and kept at 4°C for standby.

example 2

[0026] The solid fermentation medium used in the present invention is: bran: soybean meal: ammonium sulfate: sodium nitrate=1: 0.025: 0.01: 0.035; the ratio of culture medium to water is 1: 1.5w / v, and the initial pH value is 5.0-5.5 ; Sterilize at 121°C for 30 minutes, inoculum size 10 5 spores / g dry basis; the culture temperature is 36°C, and the culture time is 72h.

example 3

[0028] After the above-mentioned solid fermentation is completed, the fermented product is extracted in river water at room temperature for 3 hours, the bath ratio is 1:25w / v, and the crude enzyme solution for degumming is obtained by filtration. The crude enzyme solution is sampled and diluted 200 times. The activities of pectinase and xylanase were determined by 3,5-dinitrosalicylic acid method.

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PUM

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Abstract

A de-bonding enzyme produced by Aspergillus niger HYA4 and its use in flax de-gumming are disclosed. The process is carried out by taking potato glucose agar culturing medium as tapered-land preservative culturing medium, taking solid fermenting culture medium as bran, bean dregs, ammonia sulfate and ammonium nitrate, putting ferment under room temperature, leaching by natural water stilly, filtering to obtain coarse enzyme liquid, diluting, and determining pectase and xylanase activity. It's cheap and non-toxic; has more enzyme yield, complete varieties, shorter production period, various raw material sources, more yielding rate and combing rate and less water-source pollution.

Description

technical field [0001] The invention relates to a degumming enzyme produced by Aspergillus niger, and also relates to an application of the degumming enzyme in flax degumming. Background technique [0002] Linen fabric is luxurious and elegant, comfortable to wear, and also has a natural and green fashion style. Its market sales have been very hot for many years. my country is a big planting country of fiber flax, and the planting area in 2004 has reached 160,000 hm 2 , ranking first in the world. Nevertheless, the annual output of flax fiber in my country is astonishingly low, only 40,000-50,000 tons, and the raw material gap in the industry is more than 50%. High-quality flax fiber raw materials need to be imported for a long time. [0003] The main reason why the production of flax raw materials in my country has been lagging behind is that in addition to the unsound flax breeding system, poor flax degumming technology leads to insufficient quantity of high-quality raw ...

Claims

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Application Information

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IPC IPC(8): C12N9/00C12N1/14C12S3/06D01C1/00C12R1/685
Inventor 刘晓兰郑喜群田英华冮洁吴耘红杜国军
Owner QIQIHAR UNIVERSITY
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