Pectinase for wood processing

A technology of pectinase and wood, applied in the field of pectinase, can solve problems such as no public reports

Inactive Publication Date: 2015-12-09
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The pectinase that is used for wood processing provided by the present invention, through literature search, does not see the public report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A kind of pectinase for wood processing of the present invention obtains through the following steps:

[0020] a. Starting strain: Brevibacillus agri HS021, preservation number CCTCCM2015031; preservation time: January 11, 2015, preservation unit: China Center for Type Culture Collection, preservation unit address: China, Wuhan, Wuhan University.

[0021] b. Seed culture

[0022] Seed medium composition: in g / 1000ml, pectin 4g, yeast powder 4g, malt powder 3g, agar powder 14g, mixed with distilled water to 1000ml, sterilized at 121°C for 30min in a sterilizer, then tilted Pour it into a petri dish that has been sterilized by a sterilizer at 121°C for 60 minutes and dried;

[0023] Seed cell culture: After the seed medium is cooled, inoculate the cell on the seed medium of the Petri dish with a bamboo stick, and cultivate it in an incubator at 30°C or 33°C or 37°C for 24 hours for the cultivation of the seed cell ;

[0024] c. Fermentation culture

[0025] Compositio...

Embodiment 2

[0028] Basically with embodiment 1. The difference is that the pectinase substrate in the fermentation medium is pomelo powder. The pectinase activity was tested by conventional DNS method, and the pectinase activity (u / ml) was 5.93±0.3.

Embodiment 3

[0030] Basically with embodiment 1. The difference is that the pectinase substrate in the fermentation medium is wood flour. The pectinase activity was tested by conventional DNS method, and the pectinase activity (u / ml) was 4.92±0.3.

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Abstract

The invention belongs to the technical field of biological fermentation engineering and relates to pectinase for wood processing. Brevibacillus agri HS021 with a preservation number CCTCC M2015031 is adopted for obtaining the pectinase for wood processing by means of strain culture and fermentation. The pectinase for wood processing has the advantages that firstly, endophytes which are completely adaptive to internal environments of plants are adopted as original strains for preparation of pectinase solution which is capable of giving full play to pectinase activity in an environment with pH value ranging from 4.5 to 6.0, thereby laying the foundation for implementation and promotion of biological processing of wood materials; secondly, the pectinase with high efficiency in wood processing has a certain promotion function in implementation of biological pulping, green defibration and biological textiles.

Description

Technical field: [0001] The invention relates to a pectinase used for wood processing, which belongs to the technical field of biological fermentation engineering. Background technique: [0002] Pectinase refers to the general term for a class of enzymes that can decompose pectin. Pectinase suitable for wood processing refers to that it can effectively act on wood, within the range of wood-specific acid-base environment (pH 5.0-6.0) A class of pectinases that can function efficiently. Pectinase is mainly used in beverage, wine and other food industries, but with the increasingly serious environmental pollution, biopulping, bioseparation fiber and biotextile have received increasing attention and attention, but most of the pectinases on the market are Acid pectinase for food processing and alkaline pectinase for textile industry. Wooden materials not only have their own special chemical composition, which can inhibit biological enzymes, but also have their own unique acid-b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/88C12N9/26C12N9/18C12R1/01
CPCC12N9/88C12N9/18C12N9/2408C12Y301/01011C12Y302/01067C12Y402/02002
Inventor 邹成钢李晓平乔敏吴宁王齐玮肖瑞
Owner YUNNAN UNIV
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