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Pectase for processing woods

A technology of pectinase and wood, applied in the field of pectinase, can solve problems such as no public reports

Inactive Publication Date: 2015-11-04
YUNNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The pectinase that is used for wood processing provided by the present invention, through literature search, does not see the public report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A kind of pectinase for wood processing of the present invention obtains through the following steps:

[0019] a. Starting strain: Bacillus methylotrophicus HS047, preservation number CCTCC M 2015032; preservation time: January 11, 2015, preservation unit: China Center for Type Culture Collection, preservation unit address: Wuhan, China ,Wuhan University.

[0020] b. Seed culture

[0021] Seed medium composition: in g / 1000ml, pectin 4g, yeast powder 4g, malt powder 3g, agar powder 14g, mixed with distilled water to 1000ml, sterilized at 121°C for 30min in a sterilizer, then tilted Pour it into a petri dish that has been sterilized by a sterilizer at 121°C for 60 minutes and dried;

[0022] Seed cell culture: After the seed medium is cooled, inoculate the cell on the seed medium of the Petri dish with a bamboo stick, and cultivate it in an incubator at 30°C or 33°C or 37°C for 24 hours for the cultivation of the seed cell ;

[0023] c. Fermentation culture

[0024] ...

Embodiment 2

[0026] Basically with embodiment 1. The difference is that the pectinase substrate in the fermentation medium is bran powder or pomelo powder. The pectinase activity was tested by conventional DNS method, and the pectinase activity (u / ml) was 8.73±0.3.

Embodiment 3

[0028] Basically with embodiment 1. The difference is that the pectinase substrate in the fermentation medium is wood flour. The pectinase activity was tested by conventional DNS method, and the pectinase activity (u / ml) was 0.32±0.3.

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Abstract

The invention relates to a pectase for processing woods, belonging to the technical field of biological fermentation engineering. The pectase for processing woods is obtained through carrying out seed thallus culture and fermentation culture on Bacillus methylotrophicus HS047 with the collection number of CCTCC M2015032. The pectase has the advantages that 1, a pectase solution capable of sufficiently exerting the activity of the pectase in an environment with the pH value of 4.5-5.5 is prepared by taking an endophyte completely adapted to the environment inside a plant as a starting strain, so that the basis is laid for promoting the biological processing of wood materials; and 2, the pectase capable of efficiently processing the woods has a certain effect of promoting biological pulping, green fiber separation and biological spinning.

Description

Technical field: [0001] The invention relates to a pectinase used for wood processing, which belongs to the technical field of biological fermentation engineering. Background technique: [0002] Pectinase refers to the general term for a class of enzymes that can decompose pectin. Pectinase is mainly used in food industries such as beverages and wines. However, with the increasing environmental pollution, biopulping, bioseparation fibers and biotextiles It has been paid more and more attention to, but most of the pectinases currently on the market are acid pectinases for food processing and alkaline pectinases developed for the textile industry. Wooden materials not only have their own special chemical composition, which can inhibit biological enzymes, but also have their own unique acid-base environment, which can also adversely affect biological enzymes. Endophytes are a special type of microorganisms that are fully adapted to the acid-base environment and chemical compos...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N9/26C12N9/18C12R1/07
CPCC12N9/88C12N9/18C12N9/2408C12Y301/01011C12Y302/01067C12Y402/02002
Inventor 邹成钢乔敏李晓平王齐玮吴宁肖瑞
Owner YUNNAN UNIV
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