Laccase for processing wood

A technology of laccase and wood, applied in the field of biological fermentation engineering, can solve problems such as no public reports

Inactive Publication Date: 2016-01-27
SOUTHWEST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The laccase provided by the invention is used for wood processing, through literature search, there is no public report identical with the present invention

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A kind of laccase for wood processing of the present invention obtains through the following steps:

[0018] a. Starting strain: Methylotrophic Bacillus HS047 (Bacillus methylotrophicus HS047), preservation number CCTCCNO: M2015032; preservation time: January 11, 2015, preservation unit: China Center for Typical Culture Collection, preservation unit address: China, Wuhan, Wuhan University.

[0019] b. Seed culture

[0020] Seed medium composition: in g / 1000ml, guaiacol 5g, yeast powder 5g, malt powder 3g, agar powder 14g, KCl0.3g, NaNO 3 0.2g, CaSO 4 0.5g, MgCO 3 0.1g, make up to 1000ml with distilled water, sterilize in a sterilizing pot at 121°C for 30 minutes, then pour it into a petri dish that has been sterilized in a sterilizing pot at 121°C for 60 minutes and dried;

[0021] Seed cell culture: After the seed medium is cooled, inoculate the cell on the seed medium of the Petri dish with a bamboo stick, and cultivate it in a 30°C incubator for 24 hours for the ...

Embodiment 2

[0025] Basically with embodiment 1. The difference is: the strain was irradiated with ultraviolet light for 60 minutes, and GeSO was added to the fermentation broth 4 0.25g. The test of laccase activity was carried out by conventional spectrophotometer method, and the laccase activity (u / ml) was 28.73±0.6.

Embodiment 3

[0027] Basically with embodiment 1. The difference is: GeSO is added to the fermentation broth 4 0.35g. The test of laccase activity was carried out by conventional spectrophotometer method, and the laccase activity (u / ml) was 20.19±0.7.

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Abstract

The invention relates to laccase for processing wood, and belongs to the technical field of biological fermentation engineering. Seed thalli of bacillus methylotrophicus HS047 with the preservation number CCTCC M 2015032 are cultivated and are cultivated in a fermentation manner to obtain the laccase for processing the wood. The laccase has the advantages that endophytes completely adaptive to internal environments of plants are used as original strains, so that laccase solution capable of sufficiently realizing laccase activity in environments with pH (potential of hydrogen) values of 4.5-6.5 can be prepared, and foundations can be laid for implementing and promoting biological processing of wood materials; the laccase can be efficiently used for processing the wood, and certain driving and promoting effects for driving application of the laccase to wood processing, pulping and papermaking can be realized.

Description

Technical field: [0001] The invention relates to a laccase used for wood processing and belongs to the technical field of biological fermentation engineering. Background technique: [0002] Ligninase refers to the general term for a class of enzymes that can decompose lignin. Laccase is a very important type of ligninase. It is mainly used for lignin removal and Treatment of waste and hazardous substances in environmental governance. Lignin is the second largest biomass material after cellulose in nature, and it plays a role in supporting the mechanical properties of cells in the cell wall. The useful cellulose and hemicellulose are mostly removed by physical and chemical methods. Biological enzyme is a mild and non-polluting method for effectively removing lignin. During the process of using biological enzyme to remove lignin, it will not cause any damage to chemical substances such as cellulose and hemicellulose. However, wood materials not only have their own special c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/02C12R1/07
CPCC12N9/0061C12Y110/03002
Inventor 李晓平吴章康杜官本吴宁
Owner SOUTHWEST FORESTRY UNIVERSITY
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