Complex enzyme generated by aspergillus niger and application thereof in boiling-off of flax roving
A compound enzyme and Aspergillus niger technology, which is applied in the field of compound enzymes, can solve the problems of the internal quality of fabrics, strict requirements on process conditions, and restrictions on the development speed of the industry, and achieve the advantages of convenient extraction and preparation, large enzyme production, and reduced sewage treatment costs. Effect
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example 1
[0027] Aspergillus niger HYA4 strain was used as the strain. In order to maintain the stable high-yield characteristics of the strain, potato dextrose agar medium was used as the slant preservation medium; the raw material and preparation method of the slant medium were: 200g of potatoes were peeled, cut into pieces and boiled for 30min , and then filter with gauze, add 20g of glucose and 20g of agar, and make up water to 1000ml after melting, and the pH is natural. Sterilize at 121°C for 30 minutes, place it on an inclined plane, inoculate with an inoculation loop, incubate at a constant temperature of 36°C for 5-7 days, and store it in a refrigerator at 4°C for later use.
example 2
[0029] The solid fermentation medium used in the present invention is: bran: rice straw powder: ammonium sulfate=3: 2: 0.125, pH5.0, and the culture medium water ratio is 1: 2; 121 ℃ of sterilization 30min; Culture temperature 36 ℃ , Culture time 72h.
example 3
[0031] After the above-mentioned solid fermentation is completed, the solid fermented product is extracted with tap water at room temperature, the extraction time is 1 hour, and the bath ratio is 1:10w / v, and the crude enzyme solution for flax roving scouring is obtained by filtering, and the above crude enzyme solution Samples were taken, diluted 400 times, and pectinase and cellulase activities were determined according to the 3,5-nitrosalicylic acid method.
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