Complex enzyme generated by aspergillus niger and application thereof in boiling-off of flax roving

A compound enzyme and Aspergillus niger technology, which is applied in the field of compound enzymes, can solve the problems of the internal quality of fabrics, strict requirements on process conditions, and restrictions on the development speed of the industry, and achieve the advantages of convenient extraction and preparation, large enzyme production, and reduced sewage treatment costs. Effect

Inactive Publication Date: 2010-08-18
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This traditional scouring process has many disadvantages. The first is that it consumes a lot of energy and water, and it needs to discharge a large amount of strong alkali wastewater, which not only wastes a lot of water resources, but also has serious environmental pollution problems.
The second is that the process conditions of alkali scouring are strict

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] Aspergillus niger HYA4 strain was used as the strain. In order to maintain the stable high-yield characteristics of the strain, potato dextrose agar medium was used as the slant preservation medium; the raw material and preparation method of the slant medium were: 200g of potatoes were peeled, cut into pieces and boiled for 30min , and then filter with gauze, add 20g of glucose and 20g of agar, and make up water to 1000ml after melting, and the pH is natural. Sterilize at 121°C for 30 minutes, place it on an inclined plane, inoculate with an inoculation loop, incubate at a constant temperature of 36°C for 5-7 days, and store it in a refrigerator at 4°C for later use.

example 2

[0029] The solid fermentation medium used in the present invention is: bran: rice straw powder: ammonium sulfate=3: 2: 0.125, pH5.0, and the culture medium water ratio is 1: 2; 121 ℃ of sterilization 30min; Culture temperature 36 ℃ , Culture time 72h.

example 3

[0031] After the above-mentioned solid fermentation is completed, the solid fermented product is extracted with tap water at room temperature, the extraction time is 1 hour, and the bath ratio is 1:10w / v, and the crude enzyme solution for flax roving scouring is obtained by filtering, and the above crude enzyme solution Samples were taken, diluted 400 times, and pectinase and cellulase activities were determined according to the 3,5-nitrosalicylic acid method.

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PUM

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Abstract

The invention discloses a complex enzyme generated by aspergillus niger. The complex enzyme is prepared by taking aspergillus niger HYA4 with the preserving number of CGMCCNo.1897 as a strain through incline preservation and culture, solid fermentation and culture and leaching filtration and preparation, wherein a culture medium for the solid fermentation comprises bran, straw powder and ammonium sulfate; and the invention has the advantages that the used aspergillus niger HYA4 strain is at a food safety level and has no toxicity, large enzyme yield, complete enzyme system and more extensive culture condition. The cultured complex enzyme not only has very strong pectinase activity but also has very strong cellulase activity. The used complex enzyme can enhance the split degree of flax fibre, reduces nep and hair feather obviously, lowers the strength loss of yarn, also solves injuries caused by a chlorine bleach process to a worker and the environment thoroughly and has multiple advantages of saving water and electricity, reducing the treatment cost of sewage, lowering the production cost and the like.

Description

Technical field: [0001] The invention relates to a compound enzyme produced by Aspergillus niger, and also relates to an application method of the compound enzyme in flax roving scouring. Background technique: [0002] The scouring of flax roving is the key process of flax spinning processing. The purpose is to remove natural impurities such as pectin, lignin and wax in flax roving, so that the fibers have better separation and water absorption To improve the spinnability of flax fiber. The traditional flax roving scouring process adopts chemical methods, which are realized by boiling sodium hydroxide under certain temperature and pressure conditions. This traditional scouring process has many disadvantages. The first is that it consumes a lot of energy and water, and it needs to discharge a large amount of strong alkali wastewater, which not only wastes a lot of water resources, but also has serious environmental pollution problems. The second is that the process conditio...

Claims

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Application Information

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IPC IPC(8): C12N9/42C12N9/26C12N9/24D06M16/00D06M101/06C12R1/685
Inventor 田英华郑喜群刘晓兰
Owner QIQIHAR UNIVERSITY
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